LOCUS       40924_6307        5651 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBgal, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Lin SL.
  TITLE     Promoterless lacZ reporter gene in Salmonella
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Lin SL.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-1999) Molecular and Cellular Biology, VION 
            Pharmaceuticals, Inc., 4 Science Park, New Haven, CT 06511, USA
REFERENCE   3  (bases 1 to 5651)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5651)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-OCT-1999) Molecular and Cellular Biology, VION Pharmaceuticals, 
            Inc., 4 Science Park, New Haven, CT 06511, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..63
                     /label=AspA
                     /note="AspA"
                     /regulatory_class="terminator"
     misc_feature    68..104
                     /label=polylinker
                     /note="polylinker"
     regulatory      138..141
                     /regulatory_class="ribosome_binding_site"
     CDS             163..180
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             184..216
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             220..243
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     CDS             274..3318
                     /label=lacZ
                     /note="beta-galactosidase"
     terminator      3531..3617
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3709..3736
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        3756..3847
                     /label=AmpR promoter
     CDS             3848..4705
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4879..5467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 taatcgtaca gggtagtaca aataaaaaag gcacgtcaga tgacgtgcct tttttcttgt
       61 gagcagtaag cttgcatgcc gcggccgctc tagaattcgg atcctgcagg tcgacagcaa
      121 acggtgattt tgaaaacagg agactttccc atggggggtt ctcatcatca tcatcatcat
      181 ggtatggcta gcatgactgg tggacagcaa atgggtcggg atctgtacga cgatgacgat
      241 aaggtaccta aggatcagct tggagttgat cccgtcgttt tacaacgtcg tgactgggaa
      301 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      361 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      421 tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat
      481 cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg
      541 cccatctaca ccaacgtaac ctatcccatt acggtcaatc cgccgtttgt tcccacggag
      601 aatccgacgg gttgttactc gctcacattt aatgttgatg aaagctggct acaggaaggc
      661 cagacgcgaa ttatttttga tggcgttaac tcggcgtttc atctgtggtg caacgggcgc
      721 tgggtcggtt acggccagga cagtcgtttg ccgtctgaat ttgacctgag cgcattttta
      781 cgcgccggag aaaaccgcct cgcggtgatg gtgctgcgtt ggagtgacgg cagttatctg
      841 gaagatcagg atatgtggcg gatgagcggc attttccgtg acgtctcgtt gctgcataaa
      901 ccgactacac aaatcagcga tttccatgtt gccactcgct ttaatgatga tttcagccgc
      961 gctgtactgg aggctgaagt tcagatgtgc ggcgagttgc gtgactacct acgggtaaca
     1021 gtttctttat ggcagggtga aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa
     1081 attatcgatg agcgtggtgg ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac
     1141 ccgaaactgt ggagcgccga aatcccgaat ctctatcgtg cggtggttga actgcacacc
     1201 gccgacggca cgctgattga agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt
     1261 gaaaatggtc tgctgctgct gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac
     1321 gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga cgatggtgca ggatatcctg
     1381 ctgatgaagc agaacaactt taacgccgtg cgctgttcgc attatccgaa ccatccgctg
     1441 tggtacacgc tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa tattgaaacc
     1501 cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc gctggctacc ggcgatgagc
     1561 gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg
     1621 ctggggaatg aatcaggcca cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct
     1681 gtcgatcctt cccgcccggt gcagtatgaa ggcggcggag ccgacaccac ggccaccgat
     1741 attatttgcc cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa
     1801 tggtccatca aaaaatggct ttcgctacct ggagagacgc gcccgctgat cctttgcgaa
     1861 tacgcccacg cgatgggtaa cagtcttggc ggtttcgcta aatactggca ggcgtttcgt
     1921 cagtatcccc gtttacaggg cggcttcgtc tgggactggg tggatcagtc gctgattaaa
     1981 tatgatgaaa acggcaaccc gtggtcggct tacggcggtg attttggcga tacgccgaac
     2041 gatcgccagt tctgtatgaa cggtctggtc tttgccgacc gcacgccgca tccagcgctg
     2101 acggaagcaa aacaccagca gcagtttttc cagttccgtt tatccgggca aaccatcgaa
     2161 gtgaccagcg aatacctgtt ccgtcatagc gataacgagc tcctgcactg gatggtggcg
     2221 ctggatggta agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa
     2281 cagttgattg aactgcctga actaccgcag ccggagagcg ccgggcaact ctggctcaca
     2341 gtacgcgtag tgcaaccgaa cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg
     2401 cagcagtggc gtctggcgga aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc
     2461 ccgcatctga ccaccagcga aatggatttt tgcatcgagc tgggtaataa gcgttggcaa
     2521 tttaaccgcc agtcaggctt tctttcacag atgtggattg gcgataaaaa acaactgctg
     2581 acgccgctgc gcgatcagtt cacccgtgca ccgctggata acgacattgg cgtaagtgaa
     2641 gcgacccgca ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg ccattaccag
     2701 gccgaagcag cgttgttgca gtgcacggca gatacacttg ctgatgcggt gctgattacg
     2761 accgctcacg cgtggcagca tcaggggaaa accttattta tcagccggaa aacctaccgg
     2821 attgatggta gtggtcaaat ggcgattacc gttgatgttg aagtggcgag cgatacaccg
     2881 catccggcgc ggattggcct gaactgccag ctggcgcagg tagcagagcg ggtaaactgg
     2941 ctcggattag ggccgcaaga aaactatccc gaccgcctta ctgccgcctg ttttgaccgc
     3001 tgggatctgc cattgtcaga catgtatacc ccgtacgtct tcccgagcga aaacggtctg
     3061 cgctgcggga cgcgcgaatt gaattatggc ccacaccagt ggcgcggcga cttccagttc
     3121 aacatcagcc gctacagtca acagcaactg atggaaacca gccatcgcca tctgctgcac
     3181 gcggaagaag gcacatggct gaatatcgac ggtttccata tggggattgg tggcgacgac
     3241 tcctggagcc cgtcagtatc ggcggagttc cagctgagcg ccggtcgcta ccattaccag
     3301 ttggtctggt gtcaaaaata ataaagccgg ctgttttggc ggatgagaga agattttcag
     3361 cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg
     3421 cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc
     3481 cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac
     3541 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     3601 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag
     3661 ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc
     3721 tgacggatgg cctttttgcg tttctacaaa ctctttttgt ttatttttct aaatacattc
     3781 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     3841 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     3901 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     3961 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     4021 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     4081 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     4141 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     4201 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     4261 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     4321 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     4381 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     4441 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     4501 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     4561 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     4621 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     4681 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     4741 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     4801 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     4861 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4921 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4981 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     5041 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     5101 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     5161 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5221 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     5281 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     5341 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     5401 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     5461 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     5521 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     5581 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     5641 agcggaagag c
//
