LOCUS       40924_6187       13466 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBeloBacModified, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13466)
  AUTHORS   Gillet L, Daix V, Donofrio G, Wagner M, Koszinowski UH, China B, 
            Ackermann M, Markine-Goriaynoff N, Vanderplasschen A.
  TITLE     Development of bovine herpesvirus 4 as an expression vector using 
            bacterial artificial chromosome cloning
  JOURNAL   J. Gen. Virol. 86 (PT 4), 907-917 (2005)
  PUBMED    15784885
REFERENCE   2  (bases 1 to 13466)
  AUTHORS   Markine-Goriaynoff N.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2004) Department of Infectious and Parasitic 
            Diseases, University of Liege, 20 Blv de Colonster, B43b, Liege, 
            Liege B-4000, Belgium
REFERENCE   3  (bases 1 to 13466)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13466)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Gen. 
            Virol. 86 (PT 4), 907-917 (2005)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-JUN-2004) Department of Infectious and Parasitic Diseases, 
            University of Liege, 20 Blv de Colonster, B43b, Liege, Liege B-4000,
            Belgium"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1442..1475)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        1504..1807
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1808..2011
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        2039..2057
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2080..2796
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    2922..3043
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    3343..3816
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(3892..4860)
                     /codon_start=1
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(4863..6035)
                     /codon_start=1
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    complement(6361..6611)
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(6617..7369)
                     /codon_start=1
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      complement(7460..7679)
                     /direction=LEFT
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     promoter        8606..8708
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             8709..9365
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     protein_bind    9588..9609
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9624..9654
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9662..9678
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9686..9702
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9720..9738
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     protein_bind    complement(9780..9813)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(11245..11261)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11269..11285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11293..11323)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(11338..11359)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(11647..12235)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12409..13266)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13267..13371)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctcatgggc agccgtcttg
      421 gactttggcg cggagcaatt cgttcccgag ggccgccgcg tcacctggga gacgctcatg
      481 ttccacggcc gcgacctcta ccgcatgttt gaggtgcgct cgcacgccgc ccaggcggcg
      541 cgggcgctgc gagacctggt gctgcgcaac gaaaacctgg tagacgcact ggcctcggcc
      601 gacgagtgcc taacgtggtg caagttcatc gccaccaaga acttgcgcct gcgcaccaaa
      661 gaccctatcg tggccacggc cggcgccgtg ctggaaaacc tgcggctgaa gctagcgcct
      721 tttctgcgct gctacctgcg cgggcgcggg ctaccctcgc tggaggagct ctgcgctgcg
      781 cgccggctaa gcctcgccac ctgccccgca tcgtacatgt tcgtgatgtt ggcgcgtcta
      841 agccgcgcgg tgcgctccgg cgcagagtgc gtgccgctgt tggaggtgac tgtgggcgac
      901 gcgccttttg aggagtacat ccccggggcc tgcgtggcgg ggctcatcga tgccctggac
      961 acacacaaac aggcctgcga cagcatgacg tgcaagctcg tcgctaattt caccctggtt
     1021 cccgtgtaca tgcacggaaa atacttttac tgcaacgaga tcttctaagc taataaagtc
     1081 aacctgtcaa cattgatttg aacccgatat actcgagtgt gtatagtact tgttggggac
     1141 acaggcgcgg gcgcgctatc gggcgggcgg acttgggcgg cacaagcgcg cgcggggggg
     1201 acacaacggg ccaaggcaaa taattgggcg tcggcacaaa agcgagtcaa agtcaagctc
     1261 aatgcacccg agcgcaaata gtgcctggcg gggcgcgggg ccgcgtgcct gcctgtgttg
     1321 tcggggcaag tcggctcggc gacacgcgcg cacgcaacgg gaaacagaga ggcaaaagat
     1381 gtggcgtcga ccagtactag tgaacctctt cgaacgcgta tgcatgttta aacgggacct
     1441 aataacttcg tatagcatac attatacgaa gttatattaa gggttccgga tcccccctcg
     1501 ccccgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     1561 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     1621 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     1681 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     1741 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     1801 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     1861 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     1921 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     1981 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tcgcctggta
     2041 atacgactca ctatagggac gcgatgatcc atcgccacca tggtgagcaa gggcgaggag
     2101 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     2161 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     2221 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     2281 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     2341 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     2401 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     2461 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     2521 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     2581 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     2641 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     2701 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     2761 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca
     2821 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     2881 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     2941 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     3001 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaatcaag cttgcatgcc
     3061 tgcaggtcga ctctagagga tccccgggta ccgagctcgt cgacagcgac acacttgcat
     3121 cggatgcagc ccggttaacg tgccggcacg gcctgggtaa ccaggtattt tgtccacata
     3181 accgtgcgca aaatgttgtg gataagcagg acacagcagc aatccacagc aggcatacaa
     3241 ccgcacaccg aggttactcc gttctacagg ttacgacgac atgtcaatac ttgcccttga
     3301 caggcattga tggaatcgta gtctcacgct gatagtctga tcgacaatac aagtgggacc
     3361 gtggtcccag accgataatc agaccgacaa cacgagtggg atcgtggtcc cagactaata
     3421 atcagaccga cgatacgagt gggaccgtgg tcccagacta ataatcagac cgacgatacg
     3481 agtgggaccg tggttccaga ctaataatca gaccgacgat acgagtggga ccgtggtccc
     3541 agactaataa tcagaccgac gatacgagtg ggaccatggt cccagactaa taatcagacc
     3601 gacgatacga gtgggaccgt ggtcccagtc tgattatcag accgacgata cgagtgggac
     3661 cgtggtccca gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagactaat
     3721 aatcagaccg acgatacgag tgggaccgtg gtcccagtct gattatcaga ccgacgatac
     3781 aagtggaaca gtgggcccag agagaatatt caggccagtt atgctttctg gcctgtaaca
     3841 aaggacatta agtaaagaca gataaacgta gactaaaacg tggtcgcatc agggtgctgg
     3901 cttttcaagt tccttaagaa tggcctcaat tttctctata cactcagttg gaacacgaga
     3961 cctgtccagg ttaagcacca ttttatcgcc cttatacaat actgtcgctc caggagcaaa
     4021 ctgatgtcgt gagcttaaac tagttcttga tgcagatgac gttttaagca cagaagttaa
     4081 aagagtgata acttcttcag cttcaaatat caccccagct tttttctgct catgaaggtt
     4141 agatgcctgc tgcttaagta attcctcttt atctgtaaag gctttttgaa gtgcatcacc
     4201 tgaccgggca gatagttcac cggggtgaga aaaaagagca acaactgatt taggcaattt
     4261 ggcggtgttg atacagcggg taataatctt acgtgaaata ttttccgcat cagccagcgc
     4321 agaaatattt ccagcaaatt cattctgcaa tcggcttgca taacgctgac cacgttcata
     4381 agcacttgtt gggcgataat cgttacccaa tctggataat gcagccatct gctcatcatc
     4441 cagctcgcca accagaacac gataatcact ttcggtaagt gcagcagctt tacgacggcg
     4501 actcccatcg gcaatttcta tgacaccaga tactcttcga ccgaacgccg gtgtctgttg
     4561 accagtcagt agaaaagaag ggatgagatc atccagtgcg tcctcagtaa gcagctcctg
     4621 gtcacgttca ttacctgacc atacccgaga ggtcttctca acactatcac cccggagcac
     4681 ttcaagagta aacttcacat cccgaccaca tacaggcaaa gtaatggcat taccgcgagc
     4741 cattactcct acgcgcgcaa ttaacgaatc caccatcggg gcagctggtg tcgataacga
     4801 agtatcttca accggttgag tattgagcgt atgttttgga ataacaggcg cacgcttcat
     4861 tatctaatct cccagcgtgg tttaatcaga cgatcgaaaa tttcattgca gacaggttcc
     4921 caaatagaaa gagcatttct ccaggcacca gttgaagagc gttgatcaat ggcctgttca
     4981 aaaacagttc tcatccggat ctgaccttta ccaacttcat ccgtttcacg tacaacattt
     5041 tttagaacca tgcttcccca ggcatcccga atttgctcct ccatccacgg ggactgagag
     5101 ccattactat tgctgtattt ggtaagcaaa atacgtacat caggctcgaa ccctttaaga
     5161 tcaacgttct tgagcagatc acgaagcata tcgaaaaact gcagtgcgga ggtgtagtca
     5221 aacaactcag caggcgtggg aacaatcagc acatcagcag cacatacgac attaatcgtg
     5281 ccgataccca ggttaggcgc gctgtcaata actatgacat catagtcatg agcaacagtt
     5341 tcaatggcca gtcggagcat caggtgtgga tcggtgggca gtttaccttc atcaaatttg
     5401 cccattaact cagtttcaat acggtgcaga gccagacagg aaggaataat gtcaagcccc
     5461 ggccagcaag tgggctttat tgcataagtg acatcgtcct tttccccaag atagaaaggc
     5521 aggagagtgt cttctgcatg aatatgaaga tctggtaccc atccgtgata cattgaggct
     5581 gttccctggg ggtcgttacc ttccacgagc aaaacacgta gccccttcag agccagatcc
     5641 tgagcaagat gaacagaaac tgaggttttg taaacgccac ctttatgggc agcaaccccg
     5701 atcaccggtg gaaatacgtc ttcagcacgt cgcaatcgcg taccaaacac atcacgcata
     5761 tgattaattt gttcaattgt ataaccaaca cgttgctcaa cccgtcctcg aatttccata
     5821 tccgggtgcg gtagtcgccc tgctttctcg gcatctctga tagcctgaga agaaacccca
     5881 actaaatccg ctgcttcacc tattctccag cgccgggtta ttttcctcgc ttccgggctg
     5941 tcatcattaa actgtgcaat ggcgatagcc ttcgtcattt catgaccagc gtttatgcac
     6001 tggttaagtg tttccatgag tttcattctg aacatccttt aatcattgct ttgcgttttt
     6061 ttattaaatc ttgcaattta ctgcaaagca acaacaaaat cgcaaagtca tcaaaaaacc
     6121 gcaaagttgt ttaaaataag agcaacacta caaaaggaga taagaagagc acatacctca
     6181 gtcacttatt atcactagcg ctcgccgcag ccgtgtaacc gagcatagcg agcgaactgg
     6241 cgaggaagca aagaagaact gttctgtcag atagctctta cgctcagcgc aagaagaaat
     6301 atccaccgtg ggaaaaactc caggtagagg tacacacgcg gatagccaat tcagagtaat
     6361 aaactgtgat aatcaaccct catcaatgat gacgaactaa cccccgatat caggtcacat
     6421 gacgaaggga aagagaagga aatcaactgt gacaaactgc cctcaaattt ggcttcctta
     6481 aaaattacag ttcaaaaagt atgagaaaat ccatgcaggc tgaaggaaac agcaaaactg
     6541 tgacaaatta ccctcagtag gtcagaacaa atgtgacgaa ccaccctcaa atctgtgaca
     6601 gataaccctc agactatcct gtcgtcatgg aagtgatatc gcggaaggaa aatacgatat
     6661 gagtcgtctg gcggcctttc tttttctcaa tgtatgagag gcgcattgga gttctgctgt
     6721 tgatctcatt aacacagacc tgcaggaagc ggcggcggaa gtcaggcata cgctggtaac
     6781 tttgaggcag ctggtaacgc tctatgatcc agtcgatttt cagagagacg atgcctgagc
     6841 catccggctt acgatactga cacagggatt cgtataaacg catggcatac ggattggtga
     6901 tttcttttgt ttcactaagc cgaaactgcg taaaccggtt ctgtaacccg ataaagaagg
     6961 gaatgagata tgggttgata tgtacactgt aaagccctct ggatggactg tgcgcacgtt
     7021 tgataaacca aggaaaagat tcatagcctt tttcatcgcc ggcatcctct tcagggcgat
     7081 aaaaaaccac ttccttcccc gcgaaactct tcaatgcctg ccgtatatcc ttactggctt
     7141 ccgcagaggt caatccgaat atttcagcat atttagcaac atggatctcg cagataccgt
     7201 catgttcctg tagggtgcca tcagattttc tgatctggtc aacgaacaga tacagcatac
     7261 gtttttgatc ccgggagaga ctatatgccg cctcagtgag gtcgtttgac tggacgattc
     7321 gcgggctatt tttacgtttc ttgtgattga taaccgctgt ttccgccatg acagatccat
     7381 gtgaagtgtg acaagttttt agattgtcac actaaataaa aaagagtcaa taagcaggga
     7441 taactttgtg aaaaaacagc ttcttctgag ggcaatttgt cacagggtta agggcaattt
     7501 gtcacagaca ggactgtcat ttgagggtga tttgtcacac tgaaagggca atttgtcaca
     7561 acaccttctc tagaaccagc atggataaag gcctacaagg cgctctaaaa aagaagatct
     7621 aaaaactata aaaaaaataa ttataaaaat atccccgtgg ataagtggat aaccccaagg
     7681 gaagtttttt caggcatcgt gtgtaagcag aatatataag tgctgttccc tggtgcttcc
     7741 tcgctcactc gagggcttcg ccctgtcgct cgactgcggc gagcactact ggctgtaaaa
     7801 ggacagacca catcatggtt ctgtgttcat taggttgttc tgtccattgc tgacataatc
     7861 cgctccactt caacgtaaca ccgcacgaag atttctattg ttcctgaagg catattcaaa
     7921 tcgttttcgt taccgcttgc aggcatcatg acagaacact acttcctata aacgctacac
     7981 aggctcctga gattaataat gcggatctct acgataatgg gagattttcc cgactgtttc
     8041 gttcgcttct cagtggataa cagccagctt ctctgtttaa cagacaaaaa cagcatatcc
     8101 actcagttcc acatttccat ataaaggcca aggcatttat tctcaggata attgtttcag
     8161 catcgcaacc gcatcagact ccggcatcgc aaactgcacc cggtgccggg cagccacatc
     8221 cagcgcaaaa accttcgtgt agacttccgt tgaactgatg gacttatgtc ccatcaggct
     8281 ttgcagaact ttcagcggta taccggcata cagcatgtgc atcgcatagg aatggcggaa
     8341 cgtatgtggt gtgaccggaa cagagaacgt cacaccgtca gcagcagcgg cggcaaccgc
     8401 ctccccaatc caggtcctga ccgttctgtc cgtcacttcc cagatccgcg ctttctctgt
     8461 ccttcctgtg cgacggttac gccgctccat gagcttatcg cgaataaata cctgtgacgg
     8521 aagatcactt cgcagaataa ataaatcctg gtgtccctgt tgataccggg aagccctggg
     8581 ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag aggttccaac tttcaccata
     8641 atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg
     8701 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
     8761 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
     8821 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
     8881 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga
     8941 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
     9001 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
     9061 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
     9121 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt
     9181 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
     9241 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
     9301 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
     9361 gggcgtaatt tttttaaggc agttattggt gcccttaaac gcctggttgc tacgcctgaa
     9421 taagtgataa taagcggatg aatggcagaa attcgatgat aagctgtcaa acatgagaat
     9481 tggtcgacgg cccgggcggc cgcaaggggt tcgcgttggc cgattcatta atgcagctgg
     9541 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     9601 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     9661 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcta
     9721 tttaggtgac actatagaat actcaagctt ggctggacgt aaactcctct tcagacctaa
     9781 taacttcgta tagcatacat tatacgaagt tatattaagg gttattgaat atgatcggaa
     9841 tttcgacgga acgctaaatt gccaatccca aaaagaagca aacggccagc cactacagta
     9901 gagtaagaaa cgctgcattt attaatgtat gtcccgttta aacatgcata cgcgtacacg
     9961 ggcgcccgcg cgctaaaaga acaccgagcg cacggagcta tagcgagata aggcgccggg
    10021 gctgggagcg ggcgcggcat cggcagtaca gtcacgggcg gtctcgccag cgtcgcaggg
    10081 gcacgcggcg tctccagagt cacaggcggg gagcagcgaa cagcccggcg ccgccgcttg
    10141 ctctggcgag cagaggtcca gaatggagcc ctctgctagc tggtgggggc agaaggtgcg
    10201 tgcgggccgg cgcatagacg cccggcgcag cgtggagcgg cgcgcgcggg ccgagggtgg
    10261 cggtgggaag gccgggtccg cctcgagctc gatgaggatc tcaggggtgg tgaacgtcac
    10321 cgggtccatc tgcgcgtcaa gcaccgtgac atcgaggcag tgtaggtctc gctgctgtag
    10381 tccggcagta gtaagaaagt cgtgcagtga atcggcgagg cggtgcaggc cagtggtggg
    10441 ctcgtagccg taaacggcac caccgcgcat cagatacagc ataaacttgc ggcagggcaa
    10501 gatggcatag gcgcggccaa cgaagtgcac gggtgtagtg tgggccgtgc ggccgcggcg
    10561 catgtggtgg tgcacggggc gcgtcgtcgg gcaggacaca aagacggcct cgtcgccgcc
    10621 gggcaggtcg atcgtgcgcc cggagagcgc atctagtaga acagcgtggg ctgggggcgg
    10681 accggcagca ggcgcggagg cacgggcacc catgacggcg ccgaagctag tgggagcgga
    10741 cgcccgcggt agtgttttga ggaggagcga gggaggagga gcgagggagg aggagcgagg
    10801 gaggaggagc gagggaggag gagcgaggga ggaggagcga gggaggagga gcgagggagg
    10861 aggagcgagg gaggaggagc gagggaggag gagcgaggga ggaggagcga gggaggagga
    10921 gcgagggagg aggagcgagg gaggaggagc gagggaggag gagcaatatg gcggcatgtg
    10981 cgacggtggg tatgcaaatg agaatggaat actgggcggg gtatgctaat gagtttggaa
    11041 gcaagatggc cgcgcacatg ctcggtgatc ttggccgtgt gccaacccta ccctcgcgct
    11101 ccgcgcccat cccccacccc gcccccgccc gccgcaatgc acgcgccgcg cgaaaaaaat
    11161 tttttttttc tctgcgcccc cccgcgcggg ggctgggccg actctagagg atccccgggt
    11221 accgagctcg aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    11281 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    11341 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    11401 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    11461 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    11521 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    11581 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    11641 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    11701 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    11761 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    11821 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    11881 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    11941 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    12001 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    12061 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
    12121 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    12181 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    12241 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    12301 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    12361 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    12421 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    12481 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    12541 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    12601 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    12661 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    12721 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    12781 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    12841 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    12901 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    12961 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    13021 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    13081 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    13141 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    13201 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    13261 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    13321 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    13381 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    13441 taggcgtatc acgaggccct ttcgtc
//
