LOCUS       V000222                13541 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V000222
VERSION     V000222
KEYWORDS    pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13541)
  AUTHORS   Peralta-Yahya PP, Ouellet M, Chan R, Mukhopadhyay A, Keasling JD,
            Lee TS
  TITLE     Identification and microbial production of a terpene-based advanced
            biofuel.
  JOURNAL   Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
   PUBMED   21952217
REFERENCE   2  (bases 1 to 13541)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 13541)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Commun."; date: "2011-09-27"; pages: "
            10.1038/ncomms1494"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(15..36)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(52..1131)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(1132..1209)
                     /label="lacI promoter"
     primer_bind     1415..1434
                     /label="pBRrevBam"
                     /note="pBR322 vectors, tet region, downstream of BamHI,
                     reverse primer"
     promoter        1520..1550
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    1558..1574
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1614..2795
                     /gene="atoB"
                     /label="Acetyl-CoA acetyltransferase"
                     /note="Acetyl-CoA acetyltransferase from Escherichia coli
                     (strain K12). Accession#: P76461"
     CDS             2816..4288
                     /gene="ERG13"
                     /label="Hydroxymethylglutaryl-CoA synthase"
                     /note="Hydroxymethylglutaryl-CoA synthase from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P54839"
     CDS             5220..5231
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     terminator      5849..5920
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      5936..5963
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     promoter        6175..6204
                     /label="trc promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    6212..6228
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             6254..7582
                     /gene="ERG12"
                     /label="Mevalonate kinase"
                     /note="Mevalonate kinase from Saccharomyces cerevisiae
                     (strain ATCC 204508 / S288c). Accession#: P07277"
     CDS             7606..8958
                     /gene="ERG8"
                     /label="Phosphomevalonate kinase"
                     /note="Phosphomevalonate kinase from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P24521"
     CDS             8982..10169
                     /gene="MVD1"
                     /label="Diphosphomevalonate decarboxylase"
                     /note="Diphosphomevalonate decarboxylase from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P32377"
     CDS             10193..10738
                     /gene="idi"
                     /label="Isopentenyl-diphosphate Delta-isomerase"
                     /note="Isopentenyl-diphosphate Delta-isomerase from
                     Escherichia coli (strain K12 / DH10B). Accession#: B1XEH6"
     CDS             10762..11658
                     /gene="ispA"
                     /label="Farnesyl diphosphate synthase"
                     /note="Farnesyl diphosphate synthase from Escherichia coli
                     (strain K12). Accession#: P22939"
     terminator      11706..11777
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      11793..11820
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(11848..11865)
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11982..12527)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(12641..12735)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(12759..13415)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(13416..13518)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gacgtcggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
       61 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      121 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
      181 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
      241 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
      301 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt
      361 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
      421 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
      481 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
      541 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
      601 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
      661 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
      721 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
      781 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
      841 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
      901 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
      961 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     1021 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
     1081 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     1141 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
     1201 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
     1261 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
     1321 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     1381 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     1441 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
     1501 tcgtttaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggaattg
     1561 tgagcggata acaatttcag aattcaaaag atctaaagga ggccatcctg gccatgaaga
     1621 actgtgtgat tgtttctgcg gtccgcacgg cgatcggcag ctttaacggc tctttagcga
     1681 gcacctctgc aatcgatctg ggtgcgacgg tcattaaggc cgccattgaa cgcgccaaaa
     1741 tcgacagcca gcacgttgat gaggtgatca tgggcaatgt gttacaagcc ggcctgggtc
     1801 aaaacccagc gcgtcaagca ctgttaaaat ctggtctggc cgagaccgtg tgtggcttca
     1861 ccgtcaataa ggtttgcggc tctggcctga agagcgtggc cctggcagca caagcgattc
     1921 aagccggtca ggcacaaagc atcgttgcgg gtggcatgga gaacatgtct ctggcgccgt
     1981 acttattaga tgccaaagcc cgcagcggtt atcgcctggg cgatggtcag gtgtacgacg
     2041 tcatcttacg cgatggctta atgtgcgcga cccacggtta ccacatgggt attacggccg
     2101 aaaacgtggc gaaagaatac ggcattacgc gcgagatgca ggatgaatta gcactgcact
     2161 ctcagcgcaa agcagcagcc gcgatcgagt ctggtgcgtt tacggcggaa atcgtgccag
     2221 ttaacgtggt cacgcgcaag aagacgttcg ttttcagcca ggacgagttc ccgaaggcaa
     2281 acagcaccgc ggaggcctta ggtgccttac gcccagcctt tgacaaagcg ggcacggtca
     2341 ccgccggtaa tgcgagcggc atcaatgatg gtgcagcggc actggtcatc atggaagaga
     2401 gcgccgcatt agcagcgggt ctgaccccat tagcgcgcat taaatcttat gccagcggcg
     2461 gcgtcccacc agccctgatg ggcatgggtc cggtcccagc cacgcaaaaa gccctgcaat
     2521 tagcgggcct gcaactggcc gacattgatc tgatcgaggc gaacgaggcg tttgcagcgc
     2581 agttcctggc ggtgggtaag aatctgggct tcgacagcga gaaagtcaat gtgaacggtg
     2641 gcgcgattgc gttaggccat ccgattggtg caagcggcgc acgcatctta gtgacgttac
     2701 tgcacgccat gcaggcacgc gacaagacct taggcctggc gaccttatgt attggtggcg
     2761 gtcaaggtat cgccatggtg atcgaacgcc tgaactgatg aaggaggaaa gcaaaatgaa
     2821 actgagcacc aagctgtgct ggtgtggcat caagggtcgc ctgcgcccac aaaagcagca
     2881 acagctgcac aacacgaacc tgcaaatgac cgagctgaaa aagcagaaga cggccgagca
     2941 aaagacccgc ccgcagaacg ttggcatcaa gggcatccag atttatatcc cgacgcagtg
     3001 tgtcaaccaa tctgagctgg agaaattcga tggcgtcagc cagggtaagt acaccatcgg
     3061 cctgggccag accaacatga gcttcgtgaa cgaccgtgag gacatctatt ctatgagcct
     3121 gacggtgctg tctaagctga tcaagagcta caacatcgac acgaataaga tcggtcgtct
     3181 ggaggtgggt acggagacgc tgattgacaa gagcaaaagc gtgaagtctg tcttaatgca
     3241 gctgttcggc gagaacacgg atgtcgaggg tatcgacacc ctgaacgcgt gttacggcgg
     3301 caccaacgca ctgttcaata gcctgaactg gattgagagc aacgcctggg atggccgcga
     3361 tgcgatcgtc gtgtgcggcg atatcgccat ctatgacaag ggtgcggcac gtccgaccgg
     3421 cggtgcaggc accgttgcga tgtggattgg cccggacgca ccaattgtct tcgattctgt
     3481 ccgcgcgtct tacatggagc acgcctacga cttttacaag ccggacttca cgagcgaata
     3541 cccgtacgtg gacggccact tctctctgac ctgctatgtg aaggcgctgg accaggttta
     3601 taagtcttat agcaaaaagg cgatttctaa gggcctggtc agcgacccgg caggcagcga
     3661 cgccctgaac gtgctgaagt atttcgacta caacgtgttc catgtcccga cctgcaaatt
     3721 agtgaccaaa tcttatggcc gcctgttata taatgatttc cgtgccaacc cgcagctgtt
     3781 cccggaggtt gacgccgagc tggcgacgcg tgattacgac gagagcctga ccgacaagaa
     3841 catcgagaag accttcgtca acgtcgcgaa gccgttccac aaagagcgtg tggcccaaag
     3901 cctgatcgtc ccgaccaaca cgggcaacat gtataccgcg tctgtctacg cggcattcgc
     3961 gagcctgctg aattacgtcg gttctgacga cctgcagggc aagcgcgttg gcctgttcag
     4021 ctacggtagc ggcttagcgg ccagcctgta tagctgcaaa attgtcggcg acgtccagca
     4081 catcatcaag gagctggaca tcaccaacaa gctggcgaag cgcatcaccg agacgccgaa
     4141 agattacgag gcagcgatcg agttacgcga gaatgcgcat ctgaagaaga acttcaagcc
     4201 gcaaggtagc atcgagcacc tgcagagcgg cgtctactac ctgacgaaca ttgacgacaa
     4261 gttccgccgt tcttatgacg tcaaaaagta actagtagga ggaaaacatc atggtgctga
     4321 cgaacaaaac cgtcattagc ggcagcaagg tgaagtctct gagcagcgcc caaagctcta
     4381 gcagcggccc gtctagcagc agcgaggagg acgacagccg tgacattgag tctctggaca
     4441 agaagatccg cccgctggag gagttagagg ccctgctgag cagcggcaac accaagcagc
     4501 tgaagaacaa ggaagttgca gcgctggtga tccacggtaa gctgccactg tatgcgctgg
     4561 aaaagaaact gggcgatacg acgcgtgcgg tcgcggtgcg tcgcaaagcc ttaagcatct
     4621 tagcggaggc cccggtgtta gccagcgacc gcctgccgta caagaactac gactacgacc
     4681 gcgtgtttgg cgcgtgctgc gagaatgtca ttggctacat gccgttaccg gttggtgtga
     4741 tcggcccgct ggtcattgat ggcacgagct atcacattcc aatggcgacc acggaaggtt
     4801 gcttagtcgc cagcgccatg cgtggctgta aggcgattaa cgccggcggt ggcgcgacga
     4861 ccgtgttaac caaggatggt atgacgcgcg gtccggtcgt ccgcttccca acgctgaagc
     4921 gcagcggcgc gtgtaagatt tggctggatt ctgaggaggg ccaaaacgcg atcaagaaag
     4981 ccttcaactc tacgagccgt ttcgcgcgtt tacagcatat ccagacctgc ctggccggcg
     5041 acctgctgtt catgcgcttc cgcaccacca cgggcgatgc gatgggcatg aacatgatca
     5101 gcaagggcgt cgaatatagc ctgaaacaaa tggtggaaga atatggctgg gaggacatgg
     5161 aggttgtctc tgtgagcggc aactattgca ccgacaagaa gccggcagcc attaactgga
     5221 ttgagggtcg cggcaaaagc gtcgtggcag aagcgaccat cccaggcgac gtggtccgta
     5281 aggttctgaa gagcgacgtc agcgccctgg ttgagttaaa tatcgcgaaa aacctggtcg
     5341 gcagcgcgat ggcgggcagc gtgggtggct ttaacgcaca tgcagcgaat ctggttacgg
     5401 cggttttctt agccttaggt caggacccag cccaaaatgt cgagagcagc aactgcatta
     5461 ccttaatgaa agaggttgac ggtgacctgc gcatcagcgt ttctatgccg tctatcgagg
     5521 tcggcacgat cggcggcggc accgttttag aaccgcaagg tgcgatgctg gatctgctgg
     5581 gcgtgcgcgg cccacatgca acggccccag gcaccaatgc ccgccaactg gcccgtatcg
     5641 tggcctgcgc ggttctggcg ggtgagctga gcctgtgcgc cgcattagcc gcgggccatt
     5701 tagttcaatc tcacatgacc cacaaccgca agccggcaga accaaccaag ccaaataacc
     5761 tggacgcaac cgacattaac cgtctgaagg atggcagcgt cacgtgcatt aaaagctgat
     5821 gaggatcttt ttaaggatct ccaggcatca aataaaacga aaggctcagt cgaaagactg
     5881 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     5941 accttcgggt gggcctttct gcgtttatag cgaattgatc tggtttgaca gcttatcatc
     6001 gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct
     6061 gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata
     6121 atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca
     6181 attaatcatc cggctcgtat aatgtgtgga attgtgagcg gataacaatt tcaggatcta
     6241 ggaggaaata accatgtctc tgccattcct gacgtctgcg ccaggtaagg tgatcatctt
     6301 cggcgagcac tctgcggtgt acaataagcc ggccgtcgcc gcctctgtgt ctgcgttacg
     6361 cacctacctg ctgatcagcg aatcttctgc accggacacg atcgagctgg actttccgga
     6421 catcagcttc aaccacaagt ggagcatcaa cgacttcaac gcgatcacgg aggaccaggt
     6481 gaacagccaa aagctggcca aagcccagca agcaaccgac ggtctgtctc aggagctggt
     6541 gtctctgctg gacccgctgt tagcgcagtt aagcgagagc ttccattacc acgccgcgtt
     6601 ctgcttcctg tacatgttcg tttgcctgtg cccgcacgca aagaacatca agttcagcct
     6661 gaagagcacg ctgccgattg gcgcaggctt aggctctagc gcatctatca gcgtgagcct
     6721 ggcgctggcg atggcctatc tgggtggcct gattggcagc aacgacctgg agaaactgag
     6781 cgaaaacgac aagcacatcg tgaaccagtg ggcctttatc ggcgagaagt gcattcatgg
     6841 caccccgagc ggcattgaca acgcagttgc cacgtatggc aacgccctgc tgttcgagaa
     6901 agacagccac aacggcacga tcaacacgaa caacttcaag ttcctggacg acttcccggc
     6961 gatcccgatg attctgacct acacccgtat cccacgcagc accaaggatt tagtcgcccg
     7021 cgtgcgtgtt ttagtcaccg aaaagttccc ggaggtgatg aagccgatcc tggacgcgat
     7081 gggcgagtgc gcgctgcagg gtctggagat catgaccaag ctgagcaagt gcaagggcac
     7141 cgacgatgag gcggtggaga ccaacaatga gctgtacgag cagctgctgg agctgatccg
     7201 tatcaatcac ggcctgctgg tctctatcgg tgtgtctcac ccgggcctgg aactgatcaa
     7261 aaacctgagc gacgacctgc gcattggctc tacgaaatta acgggtgcag gtggcggtgg
     7321 ctgctcttta acgctgctgc gccgtgacat tacgcaggag caaatcgaca gcttcaagaa
     7381 gaagctgcag gacgacttca gctacgagac gttcgagacg gacctgggcg gcacgggctg
     7441 ttgcctgctg agcgccaaaa atctgaacaa ggacctgaag atcaaaagcc tggtgttcca
     7501 gctgttcgaa aacaagacga ccacgaagca gcagatcgac gacctgttac tgccgggtaa
     7561 caccaatctg ccgtggacgt cttaaggatc taggagggag atcatatgag cgaattacgt
     7621 gcattcagcg cgccaggtaa ggcactgctg gccggtggct acctggtgtt agacaccaag
     7681 tacgaggcgt tcgtcgtcgg cttatctgcc cgtatgcatg cagttgccca cccgtatggt
     7741 agcctgcagg gctctgacaa gttcgaagtg cgtgtgaaga gcaagcagtt caaggacggc
     7801 gagtggctgt accacattag cccaaagagc ggcttcatcc cggttagcat tggtggcagc
     7861 aagaacccat ttatcgagaa ggtcattgcc aacgtcttca gctacttcaa gccgaatatg
     7921 gacgattact gcaaccgcaa cctgttcgtc atcgacattt tcagcgacga cgcgtaccac
     7981 agccaagagg actctgttac ggagcatcgt ggtaaccgcc gcctgagctt ccacagccat
     8041 cgcattgagg aggtgccgaa gacgggtctg ggttctagcg ccggtttagt taccgtctta
     8101 acgacggcgt tagcgagctt cttcgtgagc gacctggaga acaacgtgga caagtaccgc
     8161 gaagtgattc ataacctggc gcaggtggca cattgtcagg cccaaggtaa gattggctct
     8221 ggttttgatg tggcagcggc cgcctatggc tctatccgct atcgccgctt tccgccggcc
     8281 ctgatcagca atctgccgga catcggctct gcgacgtatg gtagcaaact ggcgcatctg
     8341 gtggacgagg aggactggaa catcaccatt aagtctaatc acctgccgag cggcttaacg
     8401 ttatggatgg gcgatatcaa gaacggcagc gaaacggtta agctggtgca gaaagtgaaa
     8461 aactggtacg acagccacat gccggaaagc ctgaagattt acacggagct ggaccacgcc
     8521 aatagccgtt tcatggatgg tctgagcaag ctggaccgcc tgcacgaaac ccacgacgac
     8581 tacagcgacc aaatcttcga gagcctggag cgcaatgact gcacctgcca gaagtacccg
     8641 gagatcacgg aggtccgcga tgccgtggca acgattcgcc gtagcttccg caaaattacg
     8701 aaggagagcg gcgcggatat cgaaccaccg gtccagacgt ctctgctgga cgactgtcaa
     8761 accttaaagg gcgtgttaac gtgcctgatt ccgggcgcgg gtggttacga cgccattgcc
     8821 gtcatcacga aacaggacgt cgatctgcgc gcacaaacgg ccaacgacaa acgtttcagc
     8881 aaagtccaat ggctggatgt tacgcaggcc gactggggtg ttcgcaagga gaaggacccg
     8941 gaaacgtatc tggataagtg aggatctagg aggattatga gatgaccgtt tacacagcat
     9001 ccgttaccgc acccgtcaac atcgcaaccc ttaagtattg ggggaaaagg gacacgaagt
     9061 tgaatctgcc caccaattcg tccatatcag tgactttatc gcaagatgac ctcagaacgt
     9121 tgacctctgc ggctactgca cctgagtttg aacgcgacac tttgtggtta aatggagaac
     9181 cacacagcat cgacaatgaa agaactcaaa attgtctgcg cgacctacgc caattaagaa
     9241 aggaaatgga atcgaaggac gcctcattgc ccacattatc tcaatggaaa ctccacattg
     9301 tctccgaaaa taactttcct acagcagctg gtttagcttc ctccgctgct ggctttgctg
     9361 cattggtctc tgcaattgct aagttatacc aattaccaca gtcaacttca gaaatatcta
     9421 gaatagcaag aaaggggtct ggttcagctt gtagatcgtt gtttggcgga tacgtggcct
     9481 gggaaatggg aaaagctgaa gatggtcatg attccatggc agtacaaatc gcagacagct
     9541 ctgactggcc tcagatgaaa gcttgtgtcc tagttgtcag cgatattaaa aaggatgtga
     9601 gttccactca gggtatgcaa ttgaccgtgg caacctccga actatttaaa gaaagaattg
     9661 aacatgtcgt accaaagaga tttgaagtca tgcgtaaagc cattgttgaa aaagatttcg
     9721 ccacctttgc aaaggaaaca atgatggatt ccaactcttt ccatgccaca tgtttggact
     9781 ctttccctcc aatattctac atgaatgaca cttccaagcg tatcatcagt tggtgccaca
     9841 ccattaatca gttttacgga gaaacaatcg ttgcatacac gtttgatgca ggtccaaatg
     9901 ctgtgttgta ctacttagct gaaaatgagt cgaaactctt tgcatttatc tataaattgt
     9961 ttggctctgt tcctggatgg gacaagaaat ttactactga gcagcttgag gctttcaacc
    10021 atcaatttga atcatctaac tttactgcac gtgaattgga tcttgagttg caaaaggatg
    10081 ttgccagagt gattttaact caagtcggtt caggcccaca agaaacaaac gaatctttga
    10141 ttgacgcaaa gactggtcta ccaaaggaat aaggatctag gaggtaatga taatgcaaac
    10201 ggaacacgtc attttattga atgcacaggg agttcccacg ggtacgctgg aaaagtatgc
    10261 cgcacacacg gcagacaccc gcttacatct cgcgttctcc agttggctgt ttaatgccaa
    10321 aggacaatta ttagttaccc gccgcgcact gagcaaaaaa gcatggcctg gcgtgtggac
    10381 taactcggtt tgtgggcacc cacaactggg agaaagcaac gaagacgcag tgatccgccg
    10441 ttgccgttat gagcttggcg tggaaattac gcctcctgaa tctatctatc ctgactttcg
    10501 ctaccgcgcc accgatccga gtggcattgt ggaaaatgaa gtgtgtccgg tatttgccgc
    10561 acgcaccact agtgcgttac agatcaatga tgatgaagtg atggattatc aatggtgtga
    10621 tttagcagat gtattacacg gtattgatgc cacgccgtgg gcgttcagtc cgtggatggt
    10681 gatgcaggcg acaaatcgcg aagccagaaa acgattatct gcatttaccc agcttaaata
    10741 aggatctagg aggataaaga aatggacttt ccgcagcaac tcgaagcctg cgttaagcag
    10801 gccaaccagg cgctgagccg ttttatcgcc ccactgccct ttcagaacac tcccgtggtc
    10861 gaaaccatgc agtatggcgc attattaggt ggtaagcgcc tgcgaccttt cctggtttat
    10921 gccaccggtc atatgttcgg cgttagcaca aacacgctgg acgcacccgc tgccgccgtt
    10981 gagtgtatcc acgcttactc attaattcat gatgatttac cggcaatgga tgatgacgat
    11041 ctgcgtcgcg gtttgccaac ctgccatgtg aagtttggcg aagcaaacgc gattctcgct
    11101 ggcgacgctt tacaaacgct ggcgttctcg attttaagcg atgccgatat gccggaagtg
    11161 tcggaccgcg acagaatttc gatgatttct gaactggcga gcgccagtgg tattgccgga
    11221 atgtgcggtg gtcaggcatt agatttagac gcggaaggca aacacgtacc tctggacgcg
    11281 cttgagcgta ttcatcgtca taaaaccggc gcattgattc gcgccgccgt tcgccttggt
    11341 gcattaagcg ccggagataa aggacgtcgt gctctgccgg tactcgacaa gtatgcagag
    11401 agcatcggcc ttgccttcca ggttcaggat gacatcctgg atgtggtggg agatactgca
    11461 acgttgggaa aacgccaggg tgccgaccag caacttggta aaagtaccta ccctgcactt
    11521 ctgggtcttg agcaagcccg gaagaaagcc cgggatctga tcgacgatgc ccgtcagtcg
    11581 ctgaaacaac tggctgaaca gtcactcgat acctcggcac tggaagcgct agcggactac
    11641 atcatccagc gtaataaata aggatcctaa tgaggatcca aactcgagta aggatctcca
    11701 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
    11761 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
    11821 tttataccta gggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg
    11881 actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa
    11941 gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc
    12001 cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta
    12061 taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt
    12121 cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact
    12181 cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc
    12241 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa
    12301 agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg
    12361 ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac
    12421 ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt
    12481 tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta
    12541 ttaatcagat aaaatatttc tagatttcag tgcaatttat ctcttcaaat gtagcacctg
    12601 aagtcagccc catacgatat aagttgttac tagtgcttgg attctcacca ataaaaaacg
    12661 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
    12721 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg
    12781 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
    12841 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
    12901 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
    12961 ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag
    13021 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
    13081 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
    13141 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
    13201 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
    13261 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
    13321 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
    13381 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
    13441 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
    13501 cctcttacgt gccgatcaac gtctcatttt cgccagatat c
//
