LOCUS       40924_5669        5413 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Baculovirus transfer vector pBacPAK-His3, complete sequence, 
            beta-lactamase (bla) gene, complete cds.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Kitts PA.
  TITLE     pBacPAK-His3 transfer vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA
REFERENCE   3  (bases 1 to 5413)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5413)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303-4230, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020 
            East  Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an 
            order call  (415) 424-8222 or (800) 662-2566, extension 1. 
            International customers,  please contact your local distributor. For
            technical information, call  (415) 424-8222 or (800) 662-2566, 
            extension 3.
            This sequence has been compiled from information in the sequence 
            databases,  published literature and other sources, together with 
            partial sequences  obtained by CLONTECH. If you suspect there is an 
            error in this sequence,  please contact CLONTECH's Technical Service
            Department at (415) 424-8222 or  (800) 662-2566, extension 3 or 
            E-mail TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..5413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     223..1050
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        1054..1145
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             1155..1172
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     misc_recomb     1272..2629
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      2776..3231
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3513..3617
                     /label=AmpR promoter
     CDS             3618..4475
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4649..5237
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcgacatgct gaacaacaag attaatatgc ctccgtgtat aaaaaaaata ttgaacgatt
       61 tgaaagaaaa caatgtaccg cgcggcggta tgtacaggaa gaggtttata ctaaactgtt
      121 acattgcaaa cgtggtttcg tgtgccaagt gtgaaaaccg atgtttaatc aaggctctga
      181 cgcatttcta caaccacgac tccaagtgtg tcggtgaagt catgcacctt ttaatcaaat
      241 cccaagatgt gtataaacca ccaaactgcc aaaaaatgaa aactgtcgat aagctctgtc
      301 cgtttgctgg caactgcaag ggtctcaatc ctatttgtaa ttattgaata ataaaacaat
      361 tataaatgct aaatttgttt tttattaacg atacaaacca aacgcaacaa gaacatttgt
      421 agtattatct ataattgaaa aagcgtagtt ataatcgctg aggtaatatt taaaatcatt
      481 ttcaaatgat tcacagttaa tttgcgacaa tataatttta ttttcacata aactagacgc
      541 cttgtcgtct tcttcttcgt attccttctc tttttcattt ttctcctcat aaaaattaac
      601 atagttatta tcgtatccat atatgtatct atcgtataga gtaaattttt tgttgtcata
      661 aatatatatg tcttttttaa tggggtgtat agtaccgctg cgcatagttt ttctgtaatt
      721 tacaacagtg ctattttctg gtagttcttc ggagtgtgtt gctttaatta ttaaatttat
      781 ataatcaatg aatttgggat cgtcggtttt gtacaatatg ttgccggcat agtacgcagc
      841 ttcttctagt tcaattacac cattttttag cagcaccgga ttaacataac tttccaaaat
      901 gttgtacgaa ccgttaaaca aaaacagttc acctcccttt tctatactat tgtctgcgag
      961 cagttgtttg ttgttaaaaa taacagccat tgtaatgaga cgcacaaact aatatcacaa
     1021 actggaaatg tctatcaata tatagttgct gatatcatgg agataattaa aatgataacc
     1081 atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
     1141 taaataccat gggtcatcat catcaccatc acgggtcgac aagcttggat ccctgcaggc
     1201 ctcgagttcg aatctagaag atctggtacc gagctcgaat tcccgggcgg ccgcttaatt
     1261 aattgatccg ggttattagt acatttatta agcgctagat tctgtgcgtt gttgatttac
     1321 agacaattgt tgtacgtatt ttaataattc attaaattta taatctttag ggtggtatgt
     1381 tagagcgaaa atcaaatgat tttcagcgtc tttatatctg aatttaaata ttaaatcctc
     1441 aatagatttg taaaataggt ttcgattagt ttcaaacaag ggttgttttt ccgaaccgat
     1501 ggctggacta tctaatggat tttcgctcaa cgccacaaaa cttgccaaat cttgtagcag
     1561 caatctagct ttgtcgatat tcgtttgtgt tttgttttgt aataaaggtt cgacgtcgtt
     1621 caaaatatta tgcgcttttg tatttctttc atcactgtcg ttagtgtaca attgactcga
     1681 cgtaaacacg ttaaataacg cttggacata tttaacatcg ggcgtgttag ctttattagg
     1741 ccgattatcg tcgtcgtccc aaccctcgtc gttagaagtt gcttccgaag acgattttgc
     1801 catagccaca cgacgcctat taattgtgtc ggctaacacg tccgcgatca aatttgtagt
     1861 tgagcttttt ggaattattt ctgattgcgg gcgtttttgg gcgggtttca atctaactgt
     1921 gcccgatttt aattcagaca acacgttaga aagcgatggt gcaggcggtg gtaacatttc
     1981 agacggcaaa tctactaatg gcggcggtgg tggagctgat gataaatcta ccatcggtgg
     2041 aggcgcaggc ggggctggcg gcggaggcgg aggcggaggt ggtggcggtg atgcagacgg
     2101 cggtttaggc tcaaatgtct ctttaggcaa cacagtcggc acctcaacta ttgtactggt
     2161 ttcgggcgcc gtttttggtt tgaccggtct gagacgagtg cgattttttt cgtttctaat
     2221 agcttccaac aattgttgtc tgtcgtctaa aggtgcagcg ggttgaggtt ccgtcggcat
     2281 tggtggagcg ggcggcaatt cagacatcga tggtggtggt ggtggtggag gcgctggaat
     2341 gttaggcacg ggagaaggtg gtggcggcgg tgccgccggt ataatttgtt ctggtttagt
     2401 ttgttcgcgc acgattgtgg gcaccggcgc aggcgccgct ggctgcacaa cggaaggtcg
     2461 tctgcttcga ggcagcgctt ggggtggtgg caattcaata ttataattgg aatacaaatc
     2521 gtaaaaatct gctataagca ttgtaatttc gctatcgttt accgtgccga tatttaacaa
     2581 ccgctcaatg taagcaattg tattgtaaag agattgtctc aagctcggat cgatcccgca
     2641 cgccgataac aagccttttc atttttacta cagcattgta gtggcgagac acttcgctgt
     2701 cgtcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     2761 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     2821 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     2881 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     2941 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     3001 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     3061 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct
     3121 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     3181 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     3241 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     3301 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     3361 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     3421 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     3481 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3541 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3601 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     3661 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     3721 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     3781 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     3841 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     3901 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     3961 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     4021 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     4081 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     4141 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     4201 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     4261 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     4321 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     4381 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     4441 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     4501 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4561 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4621 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4681 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4741 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     4801 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4861 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4921 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4981 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5041 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5101 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5161 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5221 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5281 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     5341 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     5401 tgagcgagga agc
//
