LOCUS       40924_5659        5414 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Baculovirus transfer vector pBacPAK-His1, complete sequence, 
            beta-lactamase (bla) gene, complete cds.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Kitts PA.
  TITLE     pBacPAK-His1 transfer vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA
REFERENCE   3  (bases 1 to 5414)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5414)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303-4230, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020 
            East  Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an 
            order call  (415) 424-8222 or (800) 662-2566, extension 1. 
            International customers,  please contact your local distributor. For
            technical information, call  (415) 424-8222 or (800) 662-2566, 
            extension 3.
            This sequence has been compiled from information in the sequence 
            databases,  published literature and other sources, together with 
            partial sequences  obtained by CLONTECH. If you suspect there is an 
            error in this sequence,  please contact CLONTECH's Technical Service
            Department at (415) 424-8222 or  (800) 662-2566, extension 3 or 
            E-mail TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..5414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     223..1050
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        1054..1145
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             1155..1172
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     misc_recomb     1273..2630
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      2777..3232
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3514..3618
                     /label=AmpR promoter
     CDS             3619..4476
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4650..5238
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcgacatgct gaacaacaag attaatatgc ctccgtgtat aaaaaaaata ttgaacgatt
       61 tgaaagaaaa caatgtaccg cgcggcggta tgtacaggaa gaggtttata ctaaactgtt
      121 acattgcaaa cgtggtttcg tgtgccaagt gtgaaaaccg atgtttaatc aaggctctga
      181 cgcatttcta caaccacgac tccaagtgtg tcggtgaagt catgcacctt ttaatcaaat
      241 cccaagatgt gtataaacca ccaaactgcc aaaaaatgaa aactgtcgat aagctctgtc
      301 cgtttgctgg caactgcaag ggtctcaatc ctatttgtaa ttattgaata ataaaacaat
      361 tataaatgct aaatttgttt tttattaacg atacaaacca aacgcaacaa gaacatttgt
      421 agtattatct ataattgaaa aagcgtagtt ataatcgctg aggtaatatt taaaatcatt
      481 ttcaaatgat tcacagttaa tttgcgacaa tataatttta ttttcacata aactagacgc
      541 cttgtcgtct tcttcttcgt attccttctc tttttcattt ttctcctcat aaaaattaac
      601 atagttatta tcgtatccat atatgtatct atcgtataga gtaaattttt tgttgtcata
      661 aatatatatg tcttttttaa tggggtgtat agtaccgctg cgcatagttt ttctgtaatt
      721 tacaacagtg ctattttctg gtagttcttc ggagtgtgtt gctttaatta ttaaatttat
      781 ataatcaatg aatttgggat cgtcggtttt gtacaatatg ttgccggcat agtacgcagc
      841 ttcttctagt tcaattacac cattttttag cagcaccgga ttaacataac tttccaaaat
      901 gttgtacgaa ccgttaaaca aaaacagttc acctcccttt tctatactat tgtctgcgag
      961 cagttgtttg ttgttaaaaa taacagccat tgtaatgaga cgcacaaact aatatcacaa
     1021 actggaaatg tctatcaata tatagttgct gatatcatgg agataattaa aatgataacc
     1081 atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
     1141 taaataccat gggtcatcat catcaccatc acgtggtcga caagcttgga tccctgcagg
     1201 cctcgagttc gaatctagaa gatctggtac cgagctcgaa ttcccgggcg gccgcttaat
     1261 taattgatcc gggttattag tacatttatt aagcgctaga ttctgtgcgt tgttgattta
     1321 cagacaattg ttgtacgtat tttaataatt cattaaattt ataatcttta gggtggtatg
     1381 ttagagcgaa aatcaaatga ttttcagcgt ctttatatct gaatttaaat attaaatcct
     1441 caatagattt gtaaaatagg tttcgattag tttcaaacaa gggttgtttt tccgaaccga
     1501 tggctggact atctaatgga ttttcgctca acgccacaaa acttgccaaa tcttgtagca
     1561 gcaatctagc tttgtcgata ttcgtttgtg ttttgttttg taataaaggt tcgacgtcgt
     1621 tcaaaatatt atgcgctttt gtatttcttt catcactgtc gttagtgtac aattgactcg
     1681 acgtaaacac gttaaataac gcttggacat atttaacatc gggcgtgtta gctttattag
     1741 gccgattatc gtcgtcgtcc caaccctcgt cgttagaagt tgcttccgaa gacgattttg
     1801 ccatagccac acgacgccta ttaattgtgt cggctaacac gtccgcgatc aaatttgtag
     1861 ttgagctttt tggaattatt tctgattgcg ggcgtttttg ggcgggtttc aatctaactg
     1921 tgcccgattt taattcagac aacacgttag aaagcgatgg tgcaggcggt ggtaacattt
     1981 cagacggcaa atctactaat ggcggcggtg gtggagctga tgataaatct accatcggtg
     2041 gaggcgcagg cggggctggc ggcggaggcg gaggcggagg tggtggcggt gatgcagacg
     2101 gcggtttagg ctcaaatgtc tctttaggca acacagtcgg cacctcaact attgtactgg
     2161 tttcgggcgc cgtttttggt ttgaccggtc tgagacgagt gcgatttttt tcgtttctaa
     2221 tagcttccaa caattgttgt ctgtcgtcta aaggtgcagc gggttgaggt tccgtcggca
     2281 ttggtggagc gggcggcaat tcagacatcg atggtggtgg tggtggtgga ggcgctggaa
     2341 tgttaggcac gggagaaggt ggtggcggcg gtgccgccgg tataatttgt tctggtttag
     2401 tttgttcgcg cacgattgtg ggcaccggcg caggcgccgc tggctgcaca acggaaggtc
     2461 gtctgcttcg aggcagcgct tggggtggtg gcaattcaat attataattg gaatacaaat
     2521 cgtaaaaatc tgctataagc attgtaattt cgctatcgtt taccgtgccg atatttaaca
     2581 accgctcaat gtaagcaatt gtattgtaaa gagattgtct caagctcgga tcgatcccgc
     2641 acgccgataa caagcctttt catttttact acagcattgt agtggcgaga cacttcgctg
     2701 tcgtcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     2761 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     2821 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     2881 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     2941 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     3001 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3061 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc
     3121 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     3181 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
     3241 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     3301 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     3361 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     3421 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     3481 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3541 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3601 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     3661 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     3721 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3781 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     3841 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     3901 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     3961 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     4021 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     4081 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     4141 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     4201 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     4261 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     4321 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     4381 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4441 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     4501 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     4561 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4621 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4681 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4741 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     4801 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4861 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4921 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4981 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5041 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5101 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5161 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5221 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5281 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5341 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     5401 gtgagcgagg aagc
//
