LOCUS       40924_5634        6205 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Baculovirus shuttle vector pBacHTS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Choi EW, Seen DS, Song YB, Son HS, Jung NC, Huh WK, Hahn JS, Kim K, 
            Jeong JY, Lee TG.
  TITLE     AdHTS: a high-throughput system for generating recombinant 
            adenoviruses
  JOURNAL   J. Biotechnol. 162 (2-3), 246-252 (2012)
  PUBMED    23063969
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Son H.
  TITLE     BacHTS: a high-throughput system for recombinant baculovirus 
            expression
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6205)
  AUTHORS   Son H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2011) Seoul National University, BRNSCIENCE CO., 
            Ltd., R
REFERENCE   4  (bases 1 to 6205)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6205)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biotechnol. 162 (2-3), 246-252 (2012)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (26-MAY-2011) Seoul National University, BRNSCIENCE CO., Ltd., R"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     327..1154
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        1158..1249
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     protein_bind    1267..1391
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1534..1836
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1856..1980)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_recomb     2004..3361
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      3508..3963
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4245..4349
                     /label=AmpR promoter
     CDS             4350..5207
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5381..5969
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aacggctccg cccactatta atgaaattaa aaattccaat tttaaaaaac gcagcaagag
       61 aaacatttgt atgaaagaat gcgtagaagg aaagaaaaat gtcgtcgaca tgctgaacaa
      121 caagattaat atgcctccgt gtataaaaaa aatattgaac gatttgaaag aaaacaatgt
      181 accgcgcggc ggtatgtaca ggaagaggtt tatactaaac tgttacattg caaacgtggt
      241 ttcgtgtgcc aagtgtgaaa accgatgttt aatcaaggct ctgacgcatt tctacaacca
      301 cgactccaag tgtgtgggtg aagtcatgca tcttttaatc aaatcccaag atgtgtataa
      361 accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg
      421 caagggtctc aatcctattt gtaattattg aataataaaa caattataaa tgctaaattt
      481 gttttttatt aacgatacaa accaaacgca acaagaacat ttgtagtatt atctataatt
      541 gaaaacgcgt agttataatc gctgaggtaa tatttaaaat cattttcaaa tgattcacag
      601 ttaatttgcg acaatataat tttattttca cataaactag acgccttgtc gtcttcttct
      661 tcgtattcct tctctttttc atttttctcc tcataaaaat taacatagtt attatcgtat
      721 ccatatatgt atctatcgta tagagtaaat tttttgttgt cataaatata tatgtctttt
      781 ttaatggggt gtatagtacc gctgcgcata gtttttctgt aatttacaac agtgctattt
      841 tctggtagtt cttcggagtg tgttgcttta attattaaat ttatataatc aatgaatttg
      901 ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg cagcttcttc tagttcaatt
      961 acaccatttt ttagcagcac cggattaaca taactttcca aaatgttgta cgaaccgtta
     1021 aacaaaaaca gttcacctcc cttttctata ctattgtctg cgagcagttg tttgttgtta
     1081 aaaataacag ccattgtaat gagacgcaca aactaatatc acaaactgga aatgtctatc
     1141 aatatatagt tgctgatatc atggagataa ttaaaatgat aaccatctcg caaataaata
     1201 agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata cggatcccgg
     1261 ggtaccacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
     1321 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
     1381 cagtcactat ggcggccgca ttaggcaccc cttaggaatt cgcttactaa aagccagata
     1441 acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac
     1501 ccgaagtatg tcaaaaagag gtgtgcttct agaatgcagt ttaaggttta cacctataaa
     1561 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg
     1621 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa
     1681 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
     1741 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
     1801 atcaaaaacg ccattaacct gatgttctgg ggaatataga attcctcagg tcgaccatag
     1861 tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt
     1921 taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt
     1981 ctcgagttaa ttaattgatc cgggttatta gtacatttat taagcgctag attctgtgcg
     2041 ttgttgattt acagacaatt gttgtacgta ttttaataat tcattaaatt tataatcttt
     2101 agggtggtat gttagagcga aaatcaaatg attttcagcg tctttatatc tgaatttaaa
     2161 tattaaatcc tcaatagatt tgtaaaatag gtttcgatta gtttcaaaca agggttgttt
     2221 ttccgaaccg atggctggac tatctaatgg attttcgctc aacgccacaa aacttgccaa
     2281 atcttgtagc agcaatctag ctttgtcgat attcgtttgt gttttgtttt gtaataaagg
     2341 ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt tcatcactgt cgttagtgta
     2401 caattgactc gacgtaaaca cgttaaataa agcttggaca tatttaacat cgggcgtgtt
     2461 agctttatta ggccgattat cgtcgtcgtc ccaaccctcg tcgttagaag ttgcttccga
     2521 agacgatttt gccatagcca cacgacgcct attaattgtg tcggctaaca cgtccgcgat
     2581 caaatttgta gttgagcttt ttggaattat ttctgattgc gggcgttttt gggcgggttt
     2641 caatctaact gtgcccgatt ttaattcaga caacacgtta gaaagcgatg gtgcaggcgg
     2701 tggtaacatt tcagacggca aatctactaa tggcggcggt ggtggagctg atgataaatc
     2761 taccatcggt ggaggcgcag gcggggctgg cggcggaggc ggaggcggag gtggtggcgg
     2821 tgatgcagac ggcggtttag gctcaaatgt ctctttaggc aacacagtcg gcacctcaac
     2881 tattgtactg gtttcgggcg ccgtttttgg tttgaccggt ctgagacgag tgcgattttt
     2941 ttcgtttcta atagcttcca acaattgttg tctgtcgtct aaaggtgcag cgggttgagg
     3001 ttccgtcggc attggtggag cgggcggcaa ttcagacatc gatggtggtg gtggtggtgg
     3061 aggcgctgga atgttaggca cgggagaagg tggtggcggc ggtgccgccg gtataatttg
     3121 ttctggttta gtttgttcgc gcacgattgt gggcaccggc gcaggcgccg ctggctgcac
     3181 aacggaaggt cgtctgcttc gaggcagcgc ttggggtggt ggcaattcaa tattataatt
     3241 ggaatacaaa tcgtaaaaat ctgctataag cattgtaatt tcgctatcgt ttaccgtgcc
     3301 gatatttaac aaccgctcaa tgtaagcaat tgtattgtaa agagattgtc tcaagctcgg
     3361 atcgatcccg cacgccgata acaagccttt tcatttttac tacagcattg tagtggcgag
     3421 acacttcgct gtcgtcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     3481 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     3541 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     3601 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     3661 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     3721 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     3781 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     3841 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     3901 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     3961 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     4021 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     4081 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     4141 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     4201 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4261 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4321 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4381 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4441 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4501 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4561 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4621 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4681 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     4741 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4801 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4861 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     4921 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     4981 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5041 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5101 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5161 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5221 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5281 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5341 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5401 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5461 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5521 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5581 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5641 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5701 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5761 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5821 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5881 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5941 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6001 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6061 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6121 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     6181 cgcgttggcc gattcattaa tgcag
//
