LOCUS       40924_5594        7114 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBacGGWH, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7114)
  AUTHORS   Abdulrahman W, Uhring M, Kolb-Cheynel I, Garnier JM, Moras D, Rochel
            N, Busso D, Poterszman A.
  TITLE     A set of baculovirus transfer vectors for screening of affinity tags
            and parallel expression strategies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7114)
  AUTHORS   Busso D, Salim L, Poterszman A, Klein F, Trottier Y, Moras D.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2009) Structural Biology and Genomics Platform, 
            IGBMC, 1, rue Laurent Fries, Illkirch 67404, France
REFERENCE   3  (bases 1 to 7114)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7114)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-JAN-2009) Structural Biology and Genomics Platform, IGBMC, 1, 
            rue Laurent Fries, Illkirch 67404, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..117
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             180..833
                     /codon_start=1
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     protein_bind    852..976
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1001..1031
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1085..1741
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2086..2388
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2432..2556)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2570..2587
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     polyA_signal    2726..2860
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(2889..3054)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3238..3693
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3720..3824
                     /label=AmpR promoter
     CDS             3825..4682
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4856..5444
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(5747..5971)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(6041..6571)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(6760..6788)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 tacgtatact ccggaatatt aatagatcat ggagataatt aaaatgataa ccatctcgca
       61 aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatatt
      121 ccggattatt cataccgtcc caccatcggg cgcggatccg atctaccatg gacggtacca
      181 tgtcccctat actaggttat tggaaaatta agggccttgt gcaacccact cgacttcttt
      241 tggaatatct tgaagaaaaa tatgaagagc atttgtatga gcgcgatgaa ggtgataaat
      301 ggcgaaacaa aaagtttgaa ttgggtttgg agtttcccaa tcttccttat tatattgatg
      361 gtgatgttaa attaacacag tctatggcca tcatacgtta tatagctgac aagcacaaca
      421 tgttgggtgg ttgtccaaaa gagcgtgcag agatttcaat gcttgaagga gcggttttgg
      481 atattagata cggtgtttcg agaattgcat atagtaaaga ctttgaaact ctcaaagttg
      541 attttcttag caagctacct gaaatgctga aaatgttcga agatcgttta tgtcataaaa
      601 catatttaaa tggtgatcat gtaacccatc ctgacttcat gttgtatgac gctcttgatg
      661 ttgttttata catggaccca atgtgcctgg atgcgttccc aaaattagtt tgttttaaaa
      721 aacgtattga agctatccca caaattgata agtacttgaa atccagcaag tatatagcat
      781 ggcctttgca gggctggcaa gccacgtttg gtggtggcga ccatcctcca aaatcggatg
      841 gtacccccat cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata
      901 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
      961 atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg
     1021 ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc
     1081 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     1141 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     1201 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     1261 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     1321 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     1381 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     1441 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     1501 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     1561 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     1621 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
     1681 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
     1741 gtaaacgcgt ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga
     1801 tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat
     1861 gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca
     1921 tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc
     1981 tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta
     2041 ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt
     2101 tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt
     2161 gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa
     2221 gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc
     2281 accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac
     2341 cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc
     2401 tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca
     2461 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
     2521 ttacgtttct cgttcagctt tcttgtacaa agtggtgatg ggcctcgagc atcatcatca
     2581 tcatcacctc gagtagaagc ttgtcgagaa gtactagagg atcataatca gccataccac
     2641 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     2701 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     2761 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     2821 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactgc ttgagcctag
     2881 gagatccgaa ccagataagt gaaatctagt tccaaactat tttgtcattt ttaattttcg
     2941 tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc ctaaataatc
     3001 cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc cacagcgggg
     3061 catttttctt cctgttatgt ttttaatcaa acatcctgcc aactccatgt gacaaaccgt
     3121 catcttcggc tactttttct ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat
     3181 taatgtttgt aattgactga atatcaacgc ttatttgcag cctgaatggc gaatgggacg
     3241 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     3301 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     3361 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     3421 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     3481 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     3541 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     3601 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     3661 cgaattttaa caaaatatta acgtttacaa tttcaggtgg cacttttcgg ggaaatgtgc
     3721 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3781 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3841 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3901 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     3961 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4021 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     4081 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4141 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4201 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4261 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4321 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4381 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4441 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4501 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4561 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4621 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4681 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4741 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4801 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4861 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4921 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4981 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     5041 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5101 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5161 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5221 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa
     5281 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5341 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5401 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5461 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     5521 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     5581 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     5641 attttctcct tacgcatctg tgcggtattt cacaccgcag accagccgcg taacctggca
     5701 aaatcggtta cggttgagta ataaatggat gccctgcgta agcgggtgtg ggcggacaat
     5761 aaagtcttaa actgaacaaa atagatctaa actatgacaa taaagtctta aactagacag
     5821 aatagttgta aactgaaatc agtccagtta tgctgtgaaa aagcatactg gacttttgtt
     5881 atggctaaag caaactcttc attttctgaa gtgcaaattg cccgtcgtat taaagagggg
     5941 cgtggccaag ggcatggtaa agactatatt cgcggcgttg tgacaattta ccgaacaact
     6001 ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta ggtggcggta cttgggtcga
     6061 tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc actgcgggat
     6121 cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg gcctcatgct
     6181 tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg gagactgcga
     6241 gatcatagat atagatctca ctacgcggct gctcaaacct gggcagaacg taagccgcga
     6301 gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga
     6361 gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct acgtctccga
     6421 actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca gggccgagcc
     6481 tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg cgactgccct
     6541 gctgcgtaac atcgttgctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga
     6601 cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac aaaaaaacag
     6661 tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt
     6721 ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac cgaacaggct
     6781 tatgtcaact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg
     6841 ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa ggtttcggtc
     6901 tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt gctgtgcacg
     6961 gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt gccggtggtg
     7021 ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca tcgtttgttc
     7081 gcccaggact ctagctatag ttctagtggt tggc
//
