LOCUS       40924_5589        6445 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBacFGW, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Abdulrahman W, Uhring M, Kolb-Cheynel I, Garnier JM, Moras D, Rochel
            N, Busso D, Poterszman A.
  TITLE     A set of baculovirus transfer vectors for screening of affinity tags
            and parallel expression strategies
  JOURNAL   Anal. Biochem. 385 (2), 383-385 (2009)
  PUBMED    19061853
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Busso D, Salim L, Poterszman A, Moras D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2009) Structural Biology and Genomics Platform, 
            IGBMC, 1, rue Laurent Fries, Illkirch 67404, France
REFERENCE   3  (bases 1 to 6445)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6445)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal. 
            Biochem."; date: "2009"; volume: "385"; issue: "2"; pages: "383-385"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-JAN-2009) Structural Biology and Genomics Platform, IGBMC, 1, 
            rue Laurent Fries, Illkirch 67404, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..117
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             171..194
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     protein_bind    207..331
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        356..386
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             440..1096
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1441..1743
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1787..1911)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    2057..2191
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(2220..2385)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2569..3024
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3051..3155
                     /label=AmpR promoter
     CDS             3156..4013
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4187..4775
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(5078..5302)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5372..5902)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(6091..6119)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 tacgtatact ccggaatatt aatagatcat ggagataatt aaaatgataa ccatctcgca
       61 aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatatt
      121 ccggattatt cataccgtcc caccatcggg cgcggatctg atctaccatg gactacaaag
      181 acgatgacga caagggtacc cccatcacaa gtttgtacaa aaaagctgaa cgagaaacgt
      241 aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag actacataat
      301 actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac
      361 actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc
      421 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
      481 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
      541 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
      601 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg
      661 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
      721 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
      781 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
      841 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
      901 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
      961 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     1021 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     1081 gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta aaagccagat aacagtatgc
     1141 gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat
     1201 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
     1261 cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga
     1321 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
     1381 aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa
     1441 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
     1501 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
     1561 ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
     1621 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
     1681 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
     1741 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat
     1801 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
     1861 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg tgatgggcct
     1921 cgagtagaag cttgtcgaga agtactagag gatcataatc agccatacca catttgtaga
     1981 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     2041 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2101 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     2161 actcatcaat gtatcttatc atgtctggat ctgatcactg cttgagccta ggagatccga
     2221 accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc gtattagctt
     2281 acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat ccttaaaaac
     2341 tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacagcggg gcatttttct
     2401 tcctgttatg tttttaatca aacatcctgc caactccatg tgacaaaccg tcatcttcgg
     2461 ctactttttc tctgtcacag aatgaaaatt tttctgtcat ctcttcgtta ttaatgtttg
     2521 taattgactg aatatcaacg cttatttgca gcctgaatgg cgaatgggac gcgccctgta
     2581 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     2641 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     2701 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     2761 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     2821 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     2881 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     2941 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     3001 acaaaatatt aacgtttaca atttcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     3061 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     3121 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     3181 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     3241 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     3301 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     3361 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     3421 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     3481 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     3541 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     3601 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     3661 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     3721 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     3781 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     3841 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     3901 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     3961 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     4021 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     4081 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     4141 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     4201 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     4261 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     4321 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     4381 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4441 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4501 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4561 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     4621 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4681 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4741 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4801 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4861 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4921 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     4981 ttacgcatct gtgcggtatt tcacaccgca gaccagccgc gtaacctggc aaaatcggtt
     5041 acggttgagt aataaatgga tgccctgcgt aagcgggtgt gggcggacaa taaagtctta
     5101 aactgaacaa aatagatcta aactatgaca ataaagtctt aaactagaca gaatagttgt
     5161 aaactgaaat cagtccagtt atgctgtgaa aaagcatact ggacttttgt tatggctaaa
     5221 gcaaactctt cattttctga agtgcaaatt gcccgtcgta ttaaagaggg gcgtggccaa
     5281 gggcatggta aagactatat tcgcggcgtt gtgacaattt accgaacaac tccgcggccg
     5341 ggaagccgat ctcggcttga acgaattgtt aggtggcggt acttgggtcg atatcaaagt
     5401 gcatcacttc ttcccgtatg cccaactttg tatagagagc cactgcggga tcgtcaccgt
     5461 aatctgcttg cacgtagatc acataagcac caagcgcgtt ggcctcatgc ttgaggagat
     5521 tgatgagcgc ggtggcaatg ccctgcctcc ggtgctcgcc ggagactgcg agatcataga
     5581 tatagatctc actacgcggc tgctcaaacc tgggcagaac gtaagccgcg agagcgccaa
     5641 caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg agcaagttcc
     5701 cgaggtaatc ggagtccggc tgatgttggg agtaggtggc tacgtctccg aactcacgac
     5761 cgaaaagatc aagagcagcc cgcatggatt tgacttggtc agggccgagc ctacatgtgc
     5821 gaatgatgcc catacttgag ccacctaact ttgttttagg gcgactgccc tgctgcgtaa
     5881 catcgttgct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg
     5941 taacgcgctt gctgcttgga tgcccgaggc atagactgta caaaaaaaca gtcataacaa
     6001 gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag
     6061 ttgcgtgagc gcatacgcta cttgcattac agtttacgaa ccgaacaggc ttatgtcaac
     6121 tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt gggcagcagc
     6181 gaagtcgagg catttctgtc ctggctggcg aacgagcgca aggtttcggt ctccacgcat
     6241 cgtcaggcat tggcggcctt gctgttcttc tacggcaagg tgctgtgcac ggatctgccc
     6301 tggcttcagg agatcggaag acctcggccg tcgcggcgct tgccggtggt gctgaccccg
     6361 gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt cgcccaggac
     6421 tctagctata gttctagtgg ttggc
//
