LOCUS       40924_5579        6451 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBacAGW, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Abdulrahman W, Uhring M, Kolb-Cheynel I, Garnier JM, Moras D, Rochel
            N, Busso D, Poterszman A.
  TITLE     A set of baculovirus transfer vectors for screening of affinity tags
            and parallel expression strategies
  JOURNAL   Anal. Biochem. 385 (2), 383-385 (2009)
  PUBMED    19061853
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Busso D, Salim L, Poterszman A, Moras D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2009) Structural Biology and Genomics Platform, 
            IGBMC, 1, rue Laurent Fries, Illkirch 67404, France
REFERENCE   3  (bases 1 to 6451)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6451)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal. 
            Biochem."; date: "2009"; volume: "385"; issue: "2"; pages: "383-385"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-JAN-2009) Structural Biology and Genomics Platform, IGBMC, 1, 
            rue Laurent Fries, Illkirch 67404, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..117
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             174..200
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     protein_bind    213..337
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        362..392
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             446..1102
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1447..1749
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1793..1917)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    2063..2197
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(2226..2391)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2575..3030
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3057..3161
                     /label=AmpR promoter
     CDS             3162..4019
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4193..4781
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(5084..5308)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5378..5908)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(6097..6125)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 tacgtatact ccggaatatt aatagatcat ggagataatt aaaatgataa ccatctcgca
       61 aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatatt
      121 ccggattatt cataccgtcc caccatcggg cgcggatctg atctaccatg gcatacccat
      181 acgatgttcc agattatgct ggtaccccca tcacaagttt gtacaaaaaa gctgaacgag
      241 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta
      301 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg
      361 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag
      421 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
      481 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
      541 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
      601 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
      661 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
      721 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
      781 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
      841 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag
      901 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
      961 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
     1021 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg
     1081 cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca
     1141 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg
     1201 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac
     1261 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca
     1321 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga
     1381 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct
     1441 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg
     1501 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt
     1561 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat
     1621 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa
     1681 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc
     1741 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga
     1801 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa
     1861 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat
     1921 gggcctcgag tagaagcttg tcgagaagta ctagaggatc ataatcagcc ataccacatt
     1981 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     2041 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     2101 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     2161 gtccaaactc atcaatgtat cttatcatgt ctggatctga tcactgcttg agcctaggag
     2221 atccgaacca gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat
     2281 tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt
     2341 aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac agcggggcat
     2401 ttttcttcct gttatgtttt taatcaaaca tcctgccaac tccatgtgac aaaccgtcat
     2461 cttcggctac tttttctctg tcacagaatg aaaatttttc tgtcatctct tcgttattaa
     2521 tgtttgtaat tgactgaata tcaacgctta tttgcagcct gaatggcgaa tgggacgcgc
     2581 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     2641 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     2701 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     2761 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     2821 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     2881 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     2941 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     3001 attttaacaa aatattaacg tttacaattt caggtggcac ttttcgggga aatgtgcgcg
     3061 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     3121 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     3181 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     3241 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     3301 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     3361 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     3421 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     3481 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     3541 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     3601 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     3661 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     3721 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     3781 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     3841 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     3901 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     3961 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     4021 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     4081 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     4141 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4201 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     4261 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     4321 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     4381 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     4441 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     4501 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     4561 aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg
     4621 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     4681 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     4741 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     4801 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     4861 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     4921 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     4981 ttctccttac gcatctgtgc ggtatttcac accgcagacc agccgcgtaa cctggcaaaa
     5041 tcggttacgg ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc ggacaataaa
     5101 gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac tagacagaat
     5161 agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac ttttgttatg
     5221 gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa agaggggcgt
     5281 ggccaagggc atggtaaaga ctatattcgc ggcgttgtga caatttaccg aacaactccg
     5341 cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat
     5401 caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt
     5461 caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc tcatgcttga
     5521 ggagattgat gagcgcggtg gcaatgccct gcctccggtg ctcgccggag actgcgagat
     5581 catagatata gatctcacta cgcggctgct caaacctggg cagaacgtaa gccgcgagag
     5641 cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta ctacggagca
     5701 agttcccgag gtaatcggag tccggctgat gttgggagta ggtggctacg tctccgaact
     5761 cacgaccgaa aagatcaaga gcagcccgca tggatttgac ttggtcaggg ccgagcctac
     5821 atgtgcgaat gatgcccata cttgagccac ctaactttgt tttagggcga ctgccctgct
     5881 gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc
     5941 acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca
     6001 taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg
     6061 gaccagttgc gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat
     6121 gtcaactggg ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc
     6181 agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc
     6241 acgcatcgtc aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat
     6301 ctgccctggc ttcaggagat cggaagacct cggccgtcgc ggcgcttgcc ggtggtgctg
     6361 accccggatg aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc
     6421 caggactcta gctatagttc tagtggttgg c
//
