LOCUS       40924_5559        6424 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBac0GW, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Abdulrahman W, Uhring M, Kolb-Cheynel I, Garnier JM, Moras D, Rochel
            N, Busso D, Poterszman A.
  TITLE     A set of baculovirus transfer vectors for screening of affinity tags
            and parallel expression strategies
  JOURNAL   Anal. Biochem. 385 (2), 383-385 (2009)
  PUBMED    19061853
REFERENCE   2  (bases 1 to 6424)
  AUTHORS   Busso D, Salim L, Poterszman A, Moras D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2009) Structural Biology and Genomics Platform, 
            IGBMC, 1, rue Laurent Fries, Illkirch 67404, France
REFERENCE   3  (bases 1 to 6424)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6424)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal. 
            Biochem."; date: "2009"; volume: "385"; issue: "2"; pages: "383-385"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-JAN-2009) Structural Biology and Genomics Platform, IGBMC, 1, 
            rue Laurent Fries, Illkirch 67404, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..117
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     protein_bind    186..310
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        335..365
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             419..1075
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1420..1722
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1766..1890)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    2036..2170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(2199..2364)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2548..3003
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3030..3134
                     /label=AmpR promoter
     CDS             3135..3992
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4166..4754
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(5057..5281)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5351..5881)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(6070..6098)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 tacgtatact ccggaatatt aatagatcat ggagataatt aaaatgataa ccatctcgca
       61 aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatatt
      121 ccggattatt cataccgtcc caccatcggg cgcggatctg atctaccatg gacggtaccc
      181 ccatcacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata
      241 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc
      301 agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      361 taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat
      421 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca
      481 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat
      541 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca
      601 cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga
      661 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
      721 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
      781 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
      841 tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
      901 caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga
      961 caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt
     1021 cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaaac
     1081 gcgtggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg
     1141 cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg
     1201 aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg
     1261 atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg
     1321 ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa
     1381 tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc
     1441 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg
     1501 cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc
     1561 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat
     1621 atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa
     1681 aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt
     1741 atacacagcc agtctgcagg tcgaccatag tgactggata tgttgtgttt tacagtatta
     1801 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
     1861 ttctcgttca gctttcttgt acaaagtggt gatgggcctc gagtagaagc ttgtcgagaa
     1921 gtactagagg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     1981 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     2041 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     2101 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     2161 tgtctggatc tgatcactgc ttgagcctag gagatccgaa ccagataagt gaaatctagt
     2221 tccaaactat tttgtcattt ttaattttcg tattagctta cgacgctaca cccagttccc
     2281 atctattttg tcactcttcc ctaaataatc cttaaaaact ccatttccac ccctcccagt
     2341 tcccaactat tttgtccgcc cacagcgggg catttttctt cctgttatgt ttttaatcaa
     2401 acatcctgcc aactccatgt gacaaaccgt catcttcggc tactttttct ctgtcacaga
     2461 atgaaaattt ttctgtcatc tcttcgttat taatgtttgt aattgactga atatcaacgc
     2521 ttatttgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     2581 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     2641 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     2701 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     2761 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     2821 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     2881 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     2941 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     3001 tttcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3061 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3121 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     3181 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     3241 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     3301 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     3361 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     3421 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     3481 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     3541 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     3601 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     3661 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     3721 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     3781 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     3841 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     3901 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     3961 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     4021 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     4081 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     4141 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     4201 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     4261 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     4321 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     4381 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     4441 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4501 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt
     4561 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4621 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     4681 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4741 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4801 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4861 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4921 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     4981 cacaccgcag accagccgcg taacctggca aaatcggtta cggttgagta ataaatggat
     5041 gccctgcgta agcgggtgtg ggcggacaat aaagtcttaa actgaacaaa atagatctaa
     5101 actatgacaa taaagtctta aactagacag aatagttgta aactgaaatc agtccagtta
     5161 tgctgtgaaa aagcatactg gacttttgtt atggctaaag caaactcttc attttctgaa
     5221 gtgcaaattg cccgtcgtat taaagagggg cgtggccaag ggcatggtaa agactatatt
     5281 cgcggcgttg tgacaattta ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa
     5341 cgaattgtta ggtggcggta cttgggtcga tatcaaagtg catcacttct tcccgtatgc
     5401 ccaactttgt atagagagcc actgcgggat cgtcaccgta atctgcttgc acgtagatca
     5461 cataagcacc aagcgcgttg gcctcatgct tgaggagatt gatgagcgcg gtggcaatgc
     5521 cctgcctccg gtgctcgccg gagactgcga gatcatagat atagatctca ctacgcggct
     5581 gctcaaacct gggcagaacg taagccgcga gagcgccaac aaccgcttct tggtcgaagg
     5641 cagcaagcgc gatgaatgtc ttactacgga gcaagttccc gaggtaatcg gagtccggct
     5701 gatgttggga gtaggtggct acgtctccga actcacgacc gaaaagatca agagcagccc
     5761 gcatggattt gacttggtca gggccgagcc tacatgtgcg aatgatgccc atacttgagc
     5821 cacctaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctgcgtaaca
     5881 tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat
     5941 gcccgaggca tagactgtac aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
     6001 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
     6061 ttgcattaca gtttacgaac cgaacaggct tatgtcaact gggttcgtgc cttcatccgt
     6121 ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc
     6181 tggctggcga acgagcgcaa ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg
     6241 ctgttcttct acggcaaggt gctgtgcacg gatctgccct ggcttcagga gatcggaaga
     6301 cctcggccgt cgcggcgctt gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc
     6361 ggttttctgg aaggcgagca tcgtttgttc gcccaggact ctagctatag ttctagtggt
     6421 tggc
//
