LOCUS       Exported                5855 bp DNA     circular SYN 03-APR-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(795..1383)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1554..2414)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(1557..2414)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2415..2519)
                     /label=AmpR promoter
     enhancer        2710..3089
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3090..3293
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          3427..3902
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     regulatory      3970..3979
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    join(5746..5855,1..285)
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
ORIGIN
        1 aaaaaacatt tattttcatt gcaatgatgt atttaaatta tttctgaata ttttactaaa
       61 aagggaatgt gggaggtcag tgcatttaaa acataaagaa atgaagagct agttcaaacc
      121 ttgggaaaat acactatatc ttaaactcca tgaaagaagg tgaggctgca aacagctaat
      181 gcacattggc aacagcccct gatgcctatg ccttattcat ccctcagaaa aggattcaag
      241 tagaggcttg atttggaggt taaagttttg ctatgctgta ttttacatta cttattgttt
      301 tagctgtcct catgaatgtc ttttcactac ccatttgctt atcctgcatc tctcagcctt
      361 gactccactc agttctcttg cttagagata ccacctttcc cctgaagtgt tccttccatg
      421 ttttacggcg agatggtttc tcctcgcctg gccactcagc cttagttgtc tctgttgtct
      481 tatagaggtc tacttgaaga aggaaaaaca gggggcatgg tttgactgtc ctgtgagccc
      541 ttcttccctg cctcccccac tcacagtgac ccggaatccc tcgacatggc agtctagcac
      601 tagtgcggcc gcagatctgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      661 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      721 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      781 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      841 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      901 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      961 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1021 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1081 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1141 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1201 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     1261 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1321 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1381 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1441 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1501 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1561 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1621 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1681 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1741 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1801 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1861 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1921 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1981 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     2041 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     2101 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     2161 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     2221 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     2281 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     2341 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     2401 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     2461 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2521 acatttcccc gaaaagtgcc acctgacgtg gatcccctga gggggccccc atgggctaga
     2581 ggatccggcc tcggcctctg cataaataaa aaaaattagt cagccatgag cttggcccat
     2641 tgcatacgtt gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac
     2701 cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     2761 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     2821 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     2881 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     2941 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     3001 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     3061 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     3121 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     3181 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     3241 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
     3301 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
     3361 gggaccgatc cagcctcccc tcgaagctta catgtggtac cgagctcgga tcctgagaac
     3421 ttcagggtga gtctatggga cccttgatgt tttctttccc cttcttttct atggttaagt
     3481 tcatgtcata ggaaggggag aagtaacagg gtacacatat tgaccaaatc agggtaattt
     3541 tgcatttgta attttaaaaa atgctttctt cttttaatat acttttttgt ttatcttatt
     3601 tctaatactt tccctaatct ctttctttca gggcaataat gatacaatgt atcatgcctc
     3661 tttgcaccat tctaaagaat aacagtgata atttctgggt taaggcaata gcaatatttc
     3721 tgcatataaa tatttctgca tataaattgt aactgatgta agaggtttca tattgctaat
     3781 agcagctaca atccagctac cattctgctt ttattttatg gttgggataa ggctggatta
     3841 ttctgagtcc aagctaggcc cttttgctaa tcatgttcat acctcttatc ttcctcccac
     3901 agctcctggg caacgtgctg gtctgtgtgc tggcccatca ctttggcaaa gcacgtgaga
     3961 tctgaattcg ccaccatggg attcacaaca aagataatct tcttatacaa cctagtactg
     4021 gtctacgcgg ggtttgacga ccctcgcaaa gccatagaac tagtacaaaa gcgatatggc
     4081 cgaccatgcg attgcagcgg aggacaagtg tccgagcccc cgtcagacag ggtcagtcaa
     4141 gtgacttgct caggcaagac agcttactta atgcccgacc aaagatggaa atgtaagtca
     4201 attccaaaag acacctcccc aagcgggcca ctccaagagt gcccctgtaa ttcttaccag
     4261 tcctcagtac acagttcttg ttatacctca taccaacaat gcagatcagg caataagaca
     4321 tattatacgg ctactctgct aaaaacacaa actgggggca ccagtgatgt acaagtatta
     4381 ggatccacca acaaacttat acaatctccc tgtaatggca taaaagggca gtctatttgc
     4441 tggagcacta cagctcctat ccacgtctct gatggaggag gtccattaga caccacaaga
     4501 attaaaagtg ttcagagaaa actggaagaa attcataaag ccctatatcc tgaacttcag
     4561 tatcaccctt tggccatacc taaggttaga gataacctca tggtcgatgc ccagacttta
     4621 aacattctca atgccactta caacttactc ctaatgtcca acacgagcct agtggacgac
     4681 tgttggcttt gtttaaaatt aggtccccct actcccctcg caatacctaa cttcctatta
     4741 tcctacgtga ctcgctcctc ggataatatc tcttgtttaa taattccccc ccttctagtt
     4801 caaccgatgc agttttccaa ttcatcttgc ctcttttccc cctcctacaa cagtacagaa
     4861 gaaatagatc taggccatgt tgccttcagc aactgtacct ccataaccaa tgtcaccggt
     4921 cccatatgcg ctgtaaatgg ttcggtcttt ctctgtggca ataacatggc atacacttat
     4981 ctacccacga actggacggg gctttgcgtc ctagcaactc tcctccccga cattgacatc
     5041 attcccggag atgaaccggt ccccatccct gctattgatc attttatata tagacctaaa
     5101 cgggccatac agtttattcc tttactagca gggctaggga tcaccgcagc cttcacaaca
     5161 ggagctacag gcctaggtgt ctctgtgacc caatatacaa aattatctaa tcagctaatt
     5221 tctgatgtac aaatcttatc tagcaccata caagatctgc aagatcaagt agactcatta
     5281 gccgaagtgg ttctccagaa cagaaggggg ctagatctac ttacagcaga acaaggagga
     5341 atctgtttag ccctgcaaga aaaatgctgc ttttatgtta acaagtcagg gattgtgaga
     5401 gacaaaataa aaaccttaca agaagaacta gaaagacgta gaaaagatct agcttccaac
     5461 ccactttgga ctgggcttca agggctcctc ccttacctcc tgccctttct tggccctcta
     5521 cttaccctcc tgctcttact caccattggg ccgtgcattt ttaatcgatt agttcaattt
     5581 gttaaagaca ggatctcagt agtccaggct ttagtcctga ctcaacaata ccaccagcta
     5641 aaaccactag aatacgagcc ataagaattc accccaccag tgcaggctgc ctatcagaaa
     5701 gtggtggctg gtgtggctaa tgccctggcc cacaagtatc actaagctcg ctttcttgct
     5761 gtccaatttc tattaaaggt tcctttgttc cctaagtcca actactaaac tgggggatat
     5821 tatgaagggc cttgagcatc tggattctgc ctaat
//
