LOCUS       62056_4355        5865 bp DNA     circular SYN 07-JUL-2019
DEFINITION  Cloning vector pBXB1ts, complete sequence.
ACCESSION   MK756316
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Neil K, Allard N, Jordan D, Rodrigue S.
  TITLE     Assembly of large mobilizable genetic cargo by double recombinase 
            operated insertion of DNA (DROID)
  JOURNAL   Plasmid (2019) In press
  PUBMED    31247227
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Jordan D, Neil K, Rodrigue S.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2019) Biology, University of Sherbrooke, 2500 
            Boulevard de l'Universite, Sherbrooke, Quebec J1K 2R1, Canada
REFERENCE   3  (bases 1 to 5865)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid 
            (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-APR-2019) Biology, University of Sherbrooke, 2500 Boulevard de 
            l'Universite, Sherbrooke, Quebec J1K 2R1, Canada"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Ape v. 2018-05-04
            Sequencing Technology :: Putative Sequence
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(12..55)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    58..79
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     protein_bind    80..98
                     /label=tet operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     5'UTR           140..164
                     /label=6N-RBS
                     /note="6N-RBS"
     gene            165..1667
                     /gene="bxb1"
                     /label=bxb1
     CDS             165..1667
                     /codon_start=1
                     /transl_table=11
                     /gene="bxb1"
                     /product="Bxb1 integrase"
                     /label=bxb1
                     /protein_id="QDH44617.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      1695..1722
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      2017..2103
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2195..2222
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2241..2332
                     /label=AmpR promoter
     CDS             2333..3190
                     /label=AmpR
                     /note="beta-lactamase"
     oriT            complement(3364..3473)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      4213..4435
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             4483..5430
                     /label=Rep101(Ts)
                     /note="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
ORIGIN
        1 cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa
       61 ttcgcggccg cttctagagt ccctatcagt gatagagatt gacatcccta tcagtgatag
      121 agatactgag cacaagcttg cggctttcac acttgggggc tagcgtgaga gccctggtag
      181 tcatccgcct gtcccgcgtc accgatgcta cgacttcacc ggagcgtcag ctggagtctt
      241 gccagcagct ctgcgcccag cgcggctggg acgtcgtcgg ggtagcggag gatctggacg
      301 tctccggggc ggtcgatccg ttcgaccgga agcgcagacc gaacctggcc cggtggctag
      361 cgttcgagga gcaaccgttt gacgtgatcg tggcgtaccg ggtagatcgg ttgacccgat
      421 cgatccggca tcttcagcag ctggtccact gggccgagga ccacaagaag ctggtcgtct
      481 ccgcgaccga agcgcacttc gatacgacga cgccgtttgc ggcggtcgtc atcgcgctta
      541 tgggaacggt ggcgcagatg gaattagaag cgatcaaaga gcggaaccgt tcggctgcgc
      601 atttcaatat ccgcgccggg aaataccgag gatccctgcc gccgtgggga tacctgccta
      661 cgcgcgtgga cggggagtgg cggctggtgc cggaccctgt gcagcgagag cgcatcctcg
      721 aggtgtatca ccgcgtcgtc gacaaccacg agccgctgca tctggtggcc cacgacctga
      781 accggcgtgg tgtcctgtcg ccgaaggact acttcgcgca gctgcaaggc cgcgagccgc
      841 agggccggga gtggtcggct accgcgctga agcgatcgat gatctccgag gcgatgctcg
      901 ggtacgcgac tctgaacggt aagaccgtcc gagacgacga cggagccccg ctggtgcggg
      961 ctgagccgat cctgacccgt gagcagctgg aggcgctgcg cgccgagctc gtgaagacct
     1021 cccgggcgaa gcccgcggtg tctaccccgt cgctgctgct gcgggtgttg ttctgcgcgg
     1081 tgtgcgggga gcccgcgtac aagttcgccg ggggaggacg taagcacccg cgctaccgct
     1141 gccgctcgat ggggttcccg aagcactgcg ggaacggcac ggtggcgatg gccgagtggg
     1201 acgcgttctg cgaggagcag gtactggatc tgctcgggga cgcggagcgt ctggagaaag
     1261 tctgggtagc gggctcggac tccgcggtcg aactcgcgga ggtgaacgcg gagctggtgg
     1321 acctgacgtc gctgatcggc tccccggcct accgggcggg ctctccgcag cgagaagcac
     1381 tggatgcccg tattgcggcg ctggccgcgc ggcaagagga gctggagggc ctggaggctc
     1441 gcccgtctgg ctgggagtgg cgcgagaccg ggcagcggtt cggggactgg tggcgggagc
     1501 aggacaccgc ggcaaagaac acctggcttc ggtcgatgaa cgttcggctg acgttcgacg
     1561 tccgcggcgg gctgactcgc acgatcgact tcggggatct tcaggagtac gagcagcatc
     1621 tcaggctcgg cagcgtggtc gaacggctac acaccgggat gtcgtaagcg gccgcatgca
     1681 ttaataatca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag
     1741 aatataaaaa gccagattat taatccggct tttttattat ttcctgcagg gcgctcaaag
     1801 gcggtaatac ggttggctgt tttggcggat gagagaagat tttcagcctg atacagatta
     1861 aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg
     1921 tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg
     1981 ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg
     2041 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     2101 aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga
     2161 cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt
     2221 tttgcgtttc tacaaactct tttgtttatt tttctaaata cattcaaata tgtatccgct
     2281 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     2341 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2401 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     2461 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2521 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     2581 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2641 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2701 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     2761 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2821 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
     2881 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     2941 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3001 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     3061 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     3121 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3181 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttacgcgc
     3241 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3301 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3361 ccgccggcca gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga
     3421 cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggcggca
     3481 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     3541 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
     3601 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
     3661 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
     3721 agcgtcgatc cccgacagta agacgggtaa gcctgttgat gataccgctg ccttactggg
     3781 tgcattagcc agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat
     3841 cagagggcag gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca
     3901 taaaacgccc tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat
     3961 gaatccataa aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg
     4021 cagcgaactg aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa
     4081 ggaatattca gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa
     4141 ggtctgtttt gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact
     4201 gactaaagta gtgagttata cacagggctg ggatctattc tttttatctt tttttattct
     4261 ttctttattc tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta
     4321 gcggaattta cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat
     4381 ttacagatac ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc
     4441 aattggtata gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt
     4501 ttcaaagcaa atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta
     4561 attttatgct gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg
     4621 acggtatcgt tcacttataa ccaatacgtt cagatgatga acatcagtag ggaaaatgct
     4681 tatggtgtat tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat
     4741 cctttggtta aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa
     4801 aaattagaat tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc
     4861 ataaaatata atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat
     4921 gagtggttat taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc
     4981 cttgatgaat ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt
     5041 aaccaatggg ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg
     5101 gtggttgata agcgaggccg cccgactgat acgttgattt tccaagttga actagataga
     5161 caaatggatc tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata
     5221 ccaacaacca ttacatcaga ttcctaccta cataacggac taagaaaaac actacacgat
     5281 gctttaactg caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa
     5341 agtaagtatg atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta
     5401 gagaacatac tggctaaata cggaaggatc tgaggttctt atggctcttg tatctatcag
     5461 tgaagcatca agactaacaa acaaaagtag aacaactgtt caccgttaca tatcaaaggg
     5521 aaaactgtcc atatgcacag atgaaaacgg tgtaaaaaag atagatacat cagagctttt
     5581 acgagttttt ggtgcattta aagctgttca ccatgaacag atcgacaatg taacagatga
     5641 acagcatgta acacctaata gaacaggtga aaccagtaaa acaaagcaac tagaacatga
     5701 aattgaacac ctgagacaac ttgttacagc tcaacagtca cacatagaca gcctgaaaca
     5761 ggcgatgctg cttatcgaat caaagctgcc gacaacacgg gagccagtga cgcctcccgt
     5821 ggggaaaaaa tcatggcaat tctggaagaa atagcgcttt cagcc
//
