LOCUS       62056_4350        3675 bp DNA     circular SYN 07-JUL-2019
DEFINITION  Cloning vector pBXB1, complete sequence.
ACCESSION   MK756311
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Neil K, Allard N, Jordan D, Rodrigue S.
  TITLE     Assembly of large mobilizable genetic cargo by double recombinase 
            operated insertion of DNA (DROID)
  JOURNAL   Plasmid (2019) In press
  PUBMED    31247227
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Neil K, Rodrigue S.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2019) Biology, University of Sherbrooke, 2500 
            Boulevard de l'Universite, Sherbrooke, Quebec J1K 2R1, Canada
REFERENCE   3  (bases 1 to 3675)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid 
            (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-APR-2019) Biology, University of Sherbrooke, 2500 Boulevard de 
            l'Universite, Sherbrooke, Quebec J1K 2R1, Canada"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. 2018-05-04
            Sequencing Technology :: Putative Sequence
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(12..55)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    58..79
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     protein_bind    80..98
                     /label=tet operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     5'UTR           140..164
                     /label=N6-RBS
                     /note="N6-RBS"
     gene            165..1667
                     /gene="bxb1"
                     /label=bxb1
     CDS             165..1667
                     /codon_start=1
                     /transl_table=11
                     /gene="bxb1"
                     /product="Bxb1 integrase"
                     /label=bxb1
                     /protein_id="QDH44606.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      1695..1722
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1908..2496)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2670..3527)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3528..3632)
                     /label=AmpR promoter
ORIGIN
        1 cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa
       61 ttcgcggccg cttctagagt ccctatcagt gatagagatt gacatcccta tcagtgatag
      121 agatactgag cacaagcttg cggctttcac acttgggggc tagcgtgaga gccctggtag
      181 tcatccgcct gtcccgcgtc accgatgcta cgacttcacc ggagcgtcag ctggagtctt
      241 gccagcagct ctgcgcccag cgcggctggg acgtcgtcgg ggtagcggag gatctggacg
      301 tctccggggc ggtcgatccg ttcgaccgga agcgcagacc gaacctggcc cggtggctag
      361 cgttcgagga gcaaccgttt gacgtgatcg tggcgtaccg ggtagatcgg ttgacccgat
      421 cgatccggca tcttcagcag ctggtccact gggccgagga ccacaagaag ctggtcgtct
      481 ccgcgaccga agcgcacttc gatacgacga cgccgtttgc ggcggtcgtc atcgcgctta
      541 tgggaacggt ggcgcagatg gaattagaag cgatcaaaga gcggaaccgt tcggctgcgc
      601 atttcaatat ccgcgccggg aaataccgag gatccctgcc gccgtgggga tacctgccta
      661 cgcgcgtgga cggggagtgg cggctggtgc cggaccctgt gcagcgagag cgcatcctcg
      721 aggtgtatca ccgcgtcgtc gacaaccacg agccgctgca tctggtggcc cacgacctga
      781 accggcgtgg tgtcctgtcg ccgaaggact acttcgcgca gctgcaaggc cgcgagccgc
      841 agggccggga gtggtcggct accgcgctga agcgatcgat gatctccgag gcgatgctcg
      901 ggtacgcgac tctgaacggt aagaccgtcc gagacgacga cggagccccg ctggtgcggg
      961 ctgagccgat cctgacccgt gagcagctgg aggcgctgcg cgccgagctc gtgaagacct
     1021 cccgggcgaa gcccgcggtg tctaccccgt cgctgctgct gcgggtgttg ttctgcgcgg
     1081 tgtgcgggga gcccgcgtac aagttcgccg ggggaggacg taagcacccg cgctaccgct
     1141 gccgctcgat ggggttcccg aagcactgcg ggaacggcac ggtggcgatg gccgagtggg
     1201 acgcgttctg cgaggagcag gtactggatc tgctcgggga cgcggagcgt ctggagaaag
     1261 tctgggtagc gggctcggac tccgcggtcg aactcgcgga ggtgaacgcg gagctggtgg
     1321 acctgacgtc gctgatcggc tccccggcct accgggcggg ctctccgcag cgagaagcac
     1381 tggatgcccg tattgcggcg ctggccgcgc ggcaagagga gctggagggc ctggaggctc
     1441 gcccgtctgg ctgggagtgg cgcgagaccg ggcagcggtt cggggactgg tggcgggagc
     1501 aggacaccgc ggcaaagaac acctggcttc ggtcgatgaa cgttcggctg acgttcgacg
     1561 tccgcggcgg gctgactcgc acgatcgact tcggggatct tcaggagtac gagcagcatc
     1621 tcaggctcgg cagcgtggtc gaacggctac acaccgggat gtcgtaagcg gccgcatgca
     1681 ttaataatca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag
     1741 aatataaaaa gccagattat taatccggct tttttattat ttcctgcagg gcgctcaaag
     1801 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1861 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc
     1921 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1981 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2041 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2101 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2161 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2221 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2281 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2341 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2401 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2461 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2521 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2581 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2641 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2701 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2761 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2821 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2881 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2941 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3001 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3061 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3121 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3181 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3241 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3301 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3361 tcaaggatct taccgctgtt gagatccagt tcgatataac ccactcgtgc acccaactga
     3421 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3481 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3541 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3601 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3661 gtctaagaaa ccatt
//
