LOCUS       62056_4345        3747 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(522..538)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1012..1116
                     /label=AmpR promoter
     CDS             1117..1974
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPDLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2148..2736
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(3093..3747,1..56))
                     /codon_start=1
                     /label=lambda repressor
                     /note="phage lambda repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 aggcgacgtg cgtcctcaag ctgctcttgt gttaatggtt tcttttttgt gctcatacgt
       61 taaatctatc accgcaaggg ataaatatct aacaccgtgc gtgttgacta ttttacctct
      121 ggcggtgata atggttgcat gtactaagga ggttgtatgg aacaacgcat aaccctgaaa
      181 gattatgcaa tgcgctttgg gcaaaccaag acagctaaaa gatctctcac ctaccaaaca
      241 atgcccccct gcaaaaaata aattcatata aaaaacatac agataaccat ctgcggtgat
      301 aaattatctc tggcggtgtt gacataaata ccactggcgg tgatactgag cacatcagca
      361 ggacgcactg accaccatga aggtgacgct cttaaaaatt aagccctgaa gaagggcagc
      421 attcaaagca gaaggctttg gggtgtgtga tacgaaacga agcattggtt aaaaattaag
      481 gaggaattcc atggatccgt cgacctgcag ccaagcttgg cactggccgt cgttttacaa
      541 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      601 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      661 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
      721 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
      781 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
      841 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
      901 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
      961 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     1021 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1081 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     1141 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     1201 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     1261 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     1321 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     1381 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     1441 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     1501 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     1561 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggatctgaat
     1621 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     1681 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     1741 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     1801 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     1861 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     1921 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     1981 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     2041 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     2101 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2161 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2221 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2281 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2341 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2401 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2461 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2521 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2581 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2641 aacgcctggt atctttatag tcctgtcggg tttcgccaac ctctgacttg agcgtcgatt
     2701 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2761 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2821 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     2881 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccttatct ttccctttat
     2941 ttttgctgcg gtaagtcgca taaaaaccat tcttcataat tcaatccatt tactatgtta
     3001 tgttctgagg ggagtgaaaa ttcccctaat tcgatgaaga ttcttgctca attgttatca
     3061 gctatgcgcc gaccagaaca ccttgccgat cagccaaacg tctcttcagg ccactgacta
     3121 gcgataactt tccccacaac ggaacaactc tcattgcatg ggatcattgg gtactgtggg
     3181 tttagtggtt gtaaaaacac ctgaccgcta tccctgatca gtttcttgaa ggtaaactca
     3241 tcacccccaa gtctggctat gcagaaatca cctggctcaa cagcctgctc agggtcaacg
     3301 agaattaaca ttccgtcagg aaagcttggc ttggagcctg ttggtgcggt catggaatta
     3361 ccttcaacct caagccagaa tgcagaatca ctggcttttt tggttgtgct tacccatctc
     3421 tccgcatcac ctttggtaaa ggttctaagc ttaggtgaga acatccctgc ctgaacatga
     3481 gaaaaaacag ggtactcata ctcacttcta agtgacggct gcatactaac cgcttcatac
     3541 atctcgtaga tttctctggc gattgaaggg ctaaattctt caacgctaac tttgagaatt
     3601 tttgcaagca atgcggcgtt ataagcattt aatgcattga tgccattaaa taaagcacca
     3661 acgcctgact gccccatccc catcttgtct gcgacagatt cctgggataa gccaagttca
     3721 tttttctttt tttcataaat tgcttta
//
