LOCUS       Exported                3665 bp DNA     circular SYN 30-SEP-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3665)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3665)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3665)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 30, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(253..3665,1..252)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        80..130
                     /label=pL promoter
                     /note="lambda PL"
     terminator      490..576
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      668..695
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        714..805
                     /label=AmpR promoter
     CDS             806..1663
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1837..2425
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2780..3490)
                     /codon_start=1
                     /label=lambda repressor (ts)
                     /note="temperature-sensitive variant of the phage lambda 
                     repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     promoter        3531..3580
                     /label=pR promoter
                     /note="lambda PR"
ORIGIN
        1 accaaacaat gcccccctgc aaaaaataaa ttcatataaa aaacatacag ataaccatct
       61 gcggtgataa attatctctg gcggtgttga cataaatacc actggcggtg atactgagca
      121 catcagcagg acgcactgac caccatgaag gtgacgctct taaaaattaa gccctgaaga
      181 agggcagcat tcaaagcaga aggctttggg gtgtgtgata cgaaacgaag cattggttaa
      241 aaattaagga ggaattcccg gggatccgtc gacctgcagc caagcttggc tgttttggcg
      301 gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa
      361 aacagaattt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg ccgaactcag
      421 aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact
      481 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      541 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt
      601 gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa
      661 attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttttgttt
      721 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      781 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      841 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      901 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
      961 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1021 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     1081 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1141 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1201 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1261 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1321 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     1381 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1441 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1501 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     1561 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1621 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     1681 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1741 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1801 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1861 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1921 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1981 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2041 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2101 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2161 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2221 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2281 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2341 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2401 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2461 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2521 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2581 cgagtcagtg agcgaggaag cggaagagcg cttatctttc cctttatttt tgctgcggta
     2641 agtcgcataa aaaccattct tcataattca atccatttac tatgttatgt tctgagggga
     2701 gtgaaaattc ccctaattcg atgaagattc ttgctcaatt gttatcagct atgcgccgac
     2761 cagaacacct tgccgatcag ccaaacgtct cttcaggcca ctgactagcg ataactttcc
     2821 ccacaacgga acaactctca ttgcatggga tcattgggta ctgtgggttt agtggttgta
     2881 aaaacacctg accgctatcc ctgatcagtt tcttgaaggt aaactcatca cccccaagtc
     2941 tggctatgca gaaatcacct ggctcaacag cctgctcagg gtcaacgaga attaacattc
     3001 cgtcaggaaa gcttggcttg gagcctgttg gtgcggtcat ggaattacct tcaacctcaa
     3061 gccagaatgc agaatcactg gcttttttgg ttgtgcttac ccatctctcc gcatcacctt
     3121 tggtaaaggt tctaagctta ggtgagaaca tccctgcctg aacatgagaa aaaacagggt
     3181 actcatactc acttctaagt gacggctgca tactaaccgc ttcatacatc tcgtagattt
     3241 ctctggcgat tgaagggcta aattcttcaa cgctaacttt gagaattttt gtaagcaatg
     3301 cggcgttata agcatttaat gcattgatgc cattaaataa agcaccaacg cctgactgcc
     3361 ccatccccat cttgtctgcg acagattcct gggataagcc aagttcattt ttcttttttt
     3421 cataaattgc tttaaggcga cgtgcgtcct caagctgctc ttgtgttaat ggtttctttt
     3481 ttgtgctcat acgttaaatc tatcaccgca agggataaat atctaacacc gtgcgtgttg
     3541 actattttac ctctggcggt gataatggtt gcatgtacta aggaggttgt atggaacaac
     3601 gcataaccct gaaagattat gcaatgcgct ttgggcaaac caagacagct aaagatctct
     3661 cacct
//
