LOCUS       V013165                 4600 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013165
VERSION     V013165
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     sig_peptide     7..72
                     /label="pelB signal sequence"
                     /note="leader peptide for secretion"
     CDS             73..942
                     /gene="Cd274"
                     /label="Programmed cell death 1 ligand 1"
                     /note="Programmed cell death 1 ligand 1 from Mus musculus.
                     Accession#: Q9EP73"
     terminator      1173..1259
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1351..1378
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1397..1488
                     /label="AmpR promoter"
     CDS             1489..2346
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2520..3108
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3463..4173)
                     /label="lambda repressor (ts)"
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
ORIGIN
        1 gaattcatga aatacctgct gccgaccgct gctgctggtc tgctgctcct cgctgcccag
       61 ccggcgatgg ccatgcgtat cttcgctggt atcatcttca ccgcttgctg ccacctgctg
      121 cgtgctttca ccatcaccgc tccgaaagac ctgtacgttg ttgaatacgg ttctaacgtt
      181 accatggaat gccgtttccc ggttgaacgt gaactggacc tgctggctct ggttgtttac
      241 tgggaaaaag aagacgaaca ggttatccag ttcgttgctg gtgaagaaga cctgaaaccg
      301 cagcactcta acttccgtgg tcgtgcttct ctgccgaaag accagctgct gaaaggtaac
      361 gctgctctgc agatcaccga cgttaaactg caggacgctg gtgtttactg ctgcatcatc
      421 tcttacggtg gtgctgacta caaacgtatc accctgaaag ttaacgctcc gtaccgtaaa
      481 atcaaccagc gtatctctgt tgacccggct acctctgaac acgaactgat ctgccaggct
      541 gaaggttacc cggaagctga agttatctgg accaactctg accaccagcc ggtttctggt
      601 aaacgttctg ttaccacctc tcgtaccgaa ggtatgctgc tgaacgttac ctcttctctg
      661 cgtgttaacg ctaccgctaa cgacgttttc tactgcacct tctggcgttc tcagccgggt
      721 cagaaccaca ccgctgaact gatcatcccg gaactgccgg ctacccaccc gccgcagaac
      781 cgtacccact gggttctgct gggttctatc ctgctgttcc tgatcgttgt ttctaccgtt
      841 ctgctgttcc tgcgtaaaca ggttcgtatg ctggacgttg aaaaatgcgg tgttgaagac
      901 acctcttcta aaaaccgtaa cgacacccag ttcgaagaaa cctaggatcc gtcgacctgc
      961 agccaagctt ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc
     1021 agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc
     1081 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc
     1141 tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag
     1201 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
     1261 cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc
     1321 cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg
     1381 cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1441 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     1501 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     1561 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     1621 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     1681 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     1741 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     1801 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     1861 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1921 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1981 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     2041 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2101 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2161 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     2221 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2281 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2341 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2401 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     2461 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2521 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2581 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2641 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2701 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2761 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2821 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2881 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2941 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3001 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3061 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3121 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3181 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3241 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcttatct
     3301 ttccctttat ttttgctgcg gtaagtcgca taaaaaccat tcttcataat tcaatccatt
     3361 tactatgtta tgttctgagg ggagtgaaaa ttcccctaat tcgatgaaga ttcttgctca
     3421 attgttatca gctatgcgcc gaccagaaca ccttgccgat cagccaaacg tctcttcagg
     3481 ccactgacta gcgataactt tccccacaac ggaacaactc tcattgcatg ggatcattgg
     3541 gtactgtggg tttagtggtt gtaaaaacac ctgaccgcta tccctgatca gtttcttgaa
     3601 ggtaaactca tcacccccaa gtctggctat gcagaaatca cctggctcaa cagcctgctc
     3661 agggtcaacg agaattaaca ttccgtcagg aaagcttggc ttggagcctg ttggtgcggt
     3721 catggaatta ccttcaacct caagccagaa tgcagaatca ctggcttttt tggttgtgct
     3781 tacccatctc tccgcatcac ctttggtaaa ggttctaagc ttaggtgaga acatccctgc
     3841 ctgaacatga gaaaaaacag ggtactcata ctcacttcta agtgacggct gcatactaac
     3901 cgcttcatac atctcgtaga tttctctggc gattgaaggg ctaaattctt caacgctaac
     3961 tttgagaatt tttgtaagca atgcggcgtt ataagcattt aatgcattga tgccattaaa
     4021 taaagcacca acgcctgact gccccatccc catcttgtct gcgacagatt cctgggataa
     4081 gccaagttca tttttctttt tttcataaat tgctttaagg cgacgtgcgt cctcaagctg
     4141 ctcttgtgtt aatggtttct tttttgtgct catacgttaa atctatcacc gcaagggata
     4201 aatatctaac accgtgcgtg ttgactattt tacctctggc ggtgataatg gttgcatgta
     4261 ctaaggaggt tgtatggaac aacgcataac cctgaaagat tatgcaatgc gctttgggca
     4321 aaccaagaca gctaaagatc tctcacctac caaacaatgc ccccctgcaa aaaataaatt
     4381 catataaaaa acatacagat aaccatctgc ggtgataaat tatctctggc ggtgttgaca
     4441 taaataccac tggcggtgat actgagcaca tcagcaggac gcactgacca ccatgaaggt
     4501 gacgctctta aaaattaagc cctgaagaag ggcagcattc aaagcagaag gctttggggt
     4561 gtgtgatacg aaacgaagca ttggttaaaa attaaggagg
//
