LOCUS       62056_4330        4445 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     sig_peptide     7..72
                     /label=pelB signal sequence
                     /note="leader peptide for secretion"
     CDS             769..786
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      1018..1104
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1196..1223
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1242..1333
                     /label=AmpR promoter
     CDS             1334..2191
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2365..2953
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3308..4018)
                     /codon_start=1
                     /label=lambda repressor (ts)
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 gaattcatga aatacctgct gccgaccgct gctgctggtc tgctgctcct cgctgcccag
       61 ccggcgatgg ccatgaaatg ggttaccttc atctctctgc tgttcctgtt ctcttctgct
      121 tactctggtt cttggctgct ggaagttccg aacggtccgt ggcgttctct gaccttctac
      181 ccggcttggc tgaccgtttc tgaaggtgct aacgctacct tcacctgctc tctgtctaac
      241 tggtctgaag acctgatgct gaactggaac cgtctgtctc cgtctaacca gaccgaaaaa
      301 caggctgctt tctgcaacgg tctgtctcag ccggttcagg acgctcgttt ccagatcatc
      361 cagctgccga accgtcacga cttccacatg aacatcctgg acacccgtcg taacgactct
      421 ggtatctacc tgtgcggtgc tatctctctg cacccgaaag ctaaaatcga agaatctccg
      481 ggtgctgaac tggttgttac cgaacgtatc ctggaaggtc cgcagccgca gccgaaaccg
      541 cagccgaaac cggaaccgga accgcagccg cagggtggtt ctgaagaaga cccgtgcgct
      601 tgcgaatcta tcctgaaatt cgaagctaaa gttgaaggtc tgctgcaggc tctgacccgt
      661 aaactggaag ctgtttctgg tcgtctggct gttctggaaa accgtatcat cgctgctgct
      721 ggtgctccgg ttccgtaccc ggacccgctg gaaccgcgtg gtggttctca ccaccaccac
      781 caccactaag gatccgtcga cctgcagcca agcttggctg ttttggcgga tgagagaaga
      841 ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc
      901 ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc
      961 gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa
     1021 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1081 aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg
     1141 cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag
     1201 gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat
     1261 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1321 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     1381 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     1441 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     1501 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     1561 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     1621 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     1681 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     1741 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     1801 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     1861 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     1921 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     1981 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     2041 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     2101 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     2161 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     2221 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     2281 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2341 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2401 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2461 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2521 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2581 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2641 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2701 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2761 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     2821 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2881 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2941 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     3001 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     3061 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     3121 cgaggaagcg gaagagcgct tatctttccc tttatttttg ctgcggtaag tcgcataaaa
     3181 accattcttc ataattcaat ccatttacta tgttatgttc tgaggggagt gaaaattccc
     3241 ctaattcgat gaagattctt gctcaattgt tatcagctat gcgccgacca gaacaccttg
     3301 ccgatcagcc aaacgtctct tcaggccact gactagcgat aactttcccc acaacggaac
     3361 aactctcatt gcatgggatc attgggtact gtgggtttag tggttgtaaa aacacctgac
     3421 cgctatccct gatcagtttc ttgaaggtaa actcatcacc cccaagtctg gctatgcaga
     3481 aatcacctgg ctcaacagcc tgctcagggt caacgagaat taacattccg tcaggaaagc
     3541 ttggcttgga gcctgttggt gcggtcatgg aattaccttc aacctcaagc cagaatgcag
     3601 aatcactggc ttttttggtt gtgcttaccc atctctccgc atcacctttg gtaaaggttc
     3661 taagcttagg tgagaacatc cctgcctgaa catgagaaaa aacagggtac tcatactcac
     3721 ttctaagtga cggctgcata ctaaccgctt catacatctc gtagatttct ctggcgattg
     3781 aagggctaaa ttcttcaacg ctaactttga gaatttttgt aagcaatgcg gcgttataag
     3841 catttaatgc attgatgcca ttaaataaag caccaacgcc tgactgcccc atccccatct
     3901 tgtctgcgac agattcctgg gataagccaa gttcattttt ctttttttca taaattgctt
     3961 taaggcgacg tgcgtcctca agctgctctt gtgttaatgg tttctttttt gtgctcatac
     4021 gttaaatcta tcaccgcaag ggataaatat ctaacaccgt gcgtgttgac tattttacct
     4081 ctggcggtga taatggttgc atgtactaag gaggttgtat ggaacaacgc ataaccctga
     4141 aagattatgc aatgcgcttt gggcaaacca agacagctaa agatctctca cctaccaaac
     4201 aatgcccccc tgcaaaaaat aaattcatat aaaaaacata cagataacca tctgcggtga
     4261 taaattatct ctggcggtgt tgacataaat accactggcg gtgatactga gcacatcagc
     4321 aggacgcact gaccaccatg aaggtgacgc tcttaaaaat taagccctga agaagggcag
     4381 cattcaaagc agaaggcttt ggggtgtgtg atacgaaacg aagcattggt taaaaattaa
     4441 ggagg
//
