LOCUS       V013164                 4595 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013164
VERSION     V013164
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     sig_peptide     7..72
                     /label="pelB signal sequence"
                     /note="leader peptide for secretion"
     CDS             73..936
                     /gene="Pdcd1"
                     /label="Programmed cell death protein 1"
                     /note="Programmed cell death protein 1 from Mus musculus.
                     Accession#: Q02242"
     terminator      1168..1254
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1346..1373
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1392..1483
                     /label="AmpR promoter"
     CDS             1484..2341
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2515..3103
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3458..4168)
                     /label="lambda repressor (ts)"
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
ORIGIN
        1 gaattcatga aatacctgct gccgaccgct gctgctggtc tgctgctcct cgctgcccag
       61 ccggcgatgg ccatgtgggt tcgtcaggtt ccgtggtctt tcacctgggc tgttctgcag
      121 ctgtcttggc agtctggttg gctgctggaa gttccgaacg gtccgtggcg ttctctgacc
      181 ttctacccgg cttggctgac cgtttctgaa ggtgctaacg ctaccttcac ctgctctctg
      241 tctaactggt ctgaagacct gatgctgaac tggaaccgtc tgtctccgtc taaccagacc
      301 gaaaaacagg ctgctttctg caacggtctg tctcagccgg ttcaggacgc tcgtttccag
      361 atcatccagc tgccgaaccg tcacgacttc cacatgaaca tcctggacac ccgtcgtaac
      421 gactctggta tctacctgtg cggtgctatc tctctgcacc cgaaagctaa aatcgaagaa
      481 tctccgggtg ctgaactggt tgttaccgaa cgtatcctgg aaacctctac ccgttacccg
      541 tctccgtctc cgaaaccgga aggtcgtttc cagggtatgg ttatcggtat catgtctgct
      601 ctggttggta tcccggttct gctgctgctg gcttgggctc tggctgtttt ctgctctacc
      661 tctatgtctg aagctcgtgg tgctggttct aaagacgaca ccctgaaaga agaaccgtct
      721 gctgctccgg ttccgtctgt tgcttacgaa gaactggact tccagggtcg tgaaaaaacc
      781 ccggaactgc cgaccgcttg cgttcacacc gaatacgcta ccatcgtttt caccgaaggt
      841 ctgggtgctt ctgctatggg tcgtcgtggt tctgctgacg gtctgcaggg tccgcgtccg
      901 ccgcgtcacg aagacggtca ctgctcttgg ccgctgtaag gatccgtcga cctgcagcca
      961 agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac
     1021 gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg
     1081 accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc
     1141 atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1201 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     1261 ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca
     1321 taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt
     1381 ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     1441 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     1501 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     1561 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     1621 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     1681 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     1741 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     1801 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     1861 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1921 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1981 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     2041 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     2101 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     2161 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     2221 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     2281 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     2341 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     2401 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     2461 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2521 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2581 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2641 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     2701 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2761 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2821 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2881 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2941 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3001 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     3061 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     3121 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     3181 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     3241 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgct tatctttccc
     3301 tttatttttg ctgcggtaag tcgcataaaa accattcttc ataattcaat ccatttacta
     3361 tgttatgttc tgaggggagt gaaaattccc ctaattcgat gaagattctt gctcaattgt
     3421 tatcagctat gcgccgacca gaacaccttg ccgatcagcc aaacgtctct tcaggccact
     3481 gactagcgat aactttcccc acaacggaac aactctcatt gcatgggatc attgggtact
     3541 gtgggtttag tggttgtaaa aacacctgac cgctatccct gatcagtttc ttgaaggtaa
     3601 actcatcacc cccaagtctg gctatgcaga aatcacctgg ctcaacagcc tgctcagggt
     3661 caacgagaat taacattccg tcaggaaagc ttggcttgga gcctgttggt gcggtcatgg
     3721 aattaccttc aacctcaagc cagaatgcag aatcactggc ttttttggtt gtgcttaccc
     3781 atctctccgc atcacctttg gtaaaggttc taagcttagg tgagaacatc cctgcctgaa
     3841 catgagaaaa aacagggtac tcatactcac ttctaagtga cggctgcata ctaaccgctt
     3901 catacatctc gtagatttct ctggcgattg aagggctaaa ttcttcaacg ctaactttga
     3961 gaatttttgt aagcaatgcg gcgttataag catttaatgc attgatgcca ttaaataaag
     4021 caccaacgcc tgactgcccc atccccatct tgtctgcgac agattcctgg gataagccaa
     4081 gttcattttt ctttttttca taaattgctt taaggcgacg tgcgtcctca agctgctctt
     4141 gtgttaatgg tttctttttt gtgctcatac gttaaatcta tcaccgcaag ggataaatat
     4201 ctaacaccgt gcgtgttgac tattttacct ctggcggtga taatggttgc atgtactaag
     4261 gaggttgtat ggaacaacgc ataaccctga aagattatgc aatgcgcttt gggcaaacca
     4321 agacagctaa agatctctca cctaccaaac aatgcccccc tgcaaaaaat aaattcatat
     4381 aaaaaacata cagataacca tctgcggtga taaattatct ctggcggtgt tgacataaat
     4441 accactggcg gtgatactga gcacatcagc aggacgcact gaccaccatg aaggtgacgc
     4501 tcttaaaaat taagccctga agaagggcag cattcaaagc agaaggcttt ggggtgtgtg
     4561 atacgaaacg aagcattggt taaaaattaa ggagg
//
