LOCUS       62056_4295        4368 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             434..1141
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      1367..1453
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1545..1572
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1590..1681
                     /label=AmpR promoter
     CDS             1682..2539
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2713..3301
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3658..4368)
                     /codon_start=1
                     /label=lambda repressor
                     /note="phage lambda repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 acgttaaatc tatcaccgca agggataaat atctaacacc gtgcgtgttg actattttac
       61 ctctggcggt gataatggtt gcatgtacta aggaggttgt atggaacaac gcataaccct
      121 gaaagattat gcaatgcgct ttgggcaaac caagacagct aaaagatctc tcacctacca
      181 aacaatgccc ccctgcaaaa aataaattca tataaaaaac atacagataa ccatctgcgg
      241 tgataaatta tctctggcgg tgttgacata aataccactg gcggtgatac tgagcacatc
      301 agcaggacgc actgaccacc atgaaggtga cgctcttaaa aattaagccc tgaagaaggg
      361 cagcattcaa agcagaaggc tttggggtgt gtgatacgaa acgaagcatt ggttaaaaat
      421 taaggaggaa ttcatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
      481 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
      541 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      601 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
      661 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
      721 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
      781 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
      841 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
      901 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
      961 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     1021 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     1081 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     1141 gtaagtcgac ctgcagccaa gcttggctgt tttggcggat gagagaagat tttcagcctg
     1201 atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc tcccggcagt
     1261 agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat
     1321 ggtagtgtgg ggtctcccca tgcgagagta gccaactgcc aggcatcaaa taaaacgaaa
     1381 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     1441 gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg
     1501 gcgggcagga cgcccgccat aaactgccag gcatcaaagg aatcagaagg ccatcctgac
     1561 ggatggcctt tttgcgtttc tacaaactct ttgtttattt ttctaaatac attcaaatat
     1621 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1681 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     1741 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     1801 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     1861 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     1921 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     1981 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     2041 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     2101 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     2161 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     2221 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     2281 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     2341 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     2401 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     2461 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     2521 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     2581 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     2641 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2701 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2761 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2821 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     2881 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2941 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3001 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3061 ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac
     3121 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3181 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3241 ccaacctctg acttgagcgt cgattttgtg atgctcgtca ggggggcgga gcctatggaa
     3301 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3361 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3421 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     3481 agagcgccct tatctttccc tttatttttg ctgcggtaag tcgcataaaa accattcttc
     3541 ataattcaat ccatttacta tgttatgttc tgaggggagt gaaaattccc ctaattcgat
     3601 gaagattctt gctcaattgt tatcagctat gcgccgacca gaacaccttg ccgatcagcc
     3661 aaacgtctct tcaggccact gactagcgat aactttcccc acaacggaac aactctcatt
     3721 gcatgggatc attgggtact gtgggtttag tggttgtaaa aacacctgac cgctatccct
     3781 gatcagtttc ttgaaggtaa actcatcacc cccaagtctg gctatgcaga aatcacctgg
     3841 ctcaacagcc tgctcagggt caacgagaat taacattccg tcaggaaagc ttggcttgga
     3901 gcctgttggt gcggtcatgg aattaccttc aacctcaagc cagaatgcag aatcactggc
     3961 ttttttggtt gtgcttaccc atctctccgc atcacctttg gtaaaggttc taagcttagg
     4021 tgagaacatc cctgcctgaa catgagaaaa aacagggtac tcatactcac ttctaagtga
     4081 cggctgcata ctaaccgctt catacatctc gtagatttct ctggcgattg aagggctaaa
     4141 ttcttcaacg ctaactttga gaatttttgc aagcaatgcg gcgttataag catttaatgc
     4201 attgatgcca ttaaataaag caccaacgcc tgactgcccc atccccatct tgtctgcgac
     4261 agattcctgg gataagccaa gttcattttt ctttttttca taaattgctt taaggcgacg
     4321 tgcgtcctca agctgctctt gtgttaatgg tttctttttt gtgctcat
//
