LOCUS       V013643                 5661 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013643
VERSION     V013643
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             434..1141
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             1145..2401
                     /gene="ndh"
                     /label="Type II NADH:quinone oxidoreductase"
                     /note="Type II NADH:quinone oxidoreductase from Escherichia
                     coli (strain K12). Accession#: P00393"
     terminator      2660..2746
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2838..2865
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2883..2974
                     /label="AmpR promoter"
     CDS             2975..3832
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4006..4594
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4951..5661)
                     /label="lambda repressor"
                     /note="phage lambda repressor"
ORIGIN
        1 acgttaaatc tatcaccgca agggataaat atctaacacc gtgcgtgttg actattttac
       61 ctctggcggt gataatggtt gcatgtacta aggaggttgt atggaacaac gcataaccct
      121 gaaagattat gcaatgcgct ttgggcaaac caagacagct aaaagatctc tcacctacca
      181 aacaatgccc ccctgcaaaa aataaattca tataaaaaac atacagataa ccatctgcgg
      241 tgataaatta tctctggcgg tgttgacata aataccactg gcggtgatac tgagcacatc
      301 agcaggacgc actgaccacc atgaaggtga cgctcttaaa aattaagccc tgaagaaggg
      361 cagcattcaa agcagaaggc tttggggtgt gtgatacgaa acgaagcatt ggttaaaaat
      421 taaggaggaa ttcatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
      481 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
      541 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      601 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
      661 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
      721 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
      781 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
      841 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
      901 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
      961 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     1021 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     1081 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     1141 gggaggaggg ggaagcatgg caacacagct ggggcataag ctgggacgca agaaaaaagc
     1201 caaaattacg ctggtcgatc gtaaccacag ccacctgtgg aaaccgctgc tgcacgaagt
     1261 ggcgactggc tcgcttgatg aaggcgtcga tgcgttgagc tatctggccc atgcgcgcaa
     1321 tcatggtttc cagttccagc tgggttccgt cattgatatt gatcgtgaag cgaaaacaat
     1381 cactattgca gaactgcgcg acgagaaagg tgaactgctg gttccggaac gtaaaatcgc
     1441 ctatgacacc ctggtaatgg cgctgggtag cacctctaac gatttcaata cgccaggtgt
     1501 caaagagaac tgcattttcc tcgataaccc gcaccaggcg cgtcgcttcc accaggagat
     1561 gctgaatttg ttcctgaaat actccgccaa cctgggcgcg aatggcaaag tgaacattgc
     1621 gattgtcggc ggcggcgcga cgggtgtaga actctccgct gaattgcaca acgcggtcaa
     1681 gcaactgcac agctacggtt acaaaggcct gaccaacgaa gccctgaacg taacgctggt
     1741 agaagcggga gaacgtattt tgcctgcgtt accgccacgt atctctgctg cggcccacaa
     1801 cgagctaacg aaacttggcg ttcgcgtgct gacgcaaacc atggtcacca gtgctgatga
     1861 aggcggcctg cacactaaag atggcgaata tattgaggct gatctgatgg tatgggcagc
     1921 cgggatcaaa gcgccagact tcctgaaaga tatcggtggt cttgaaacta accgtatcaa
     1981 ccagctggtg gtggaaccga cgctgcaaac cacccgcgat ccagacattt acgctattgg
     2041 cgactgcgcg tcatgcccgc gtccggaagg gggctttgtt ccgccgcgtg ctcaggctgc
     2101 acaccagatg gcgacttgcg caatgaacaa cattctggcg cagatgaacg gtaagccgct
     2161 gaaaaattat cagtataaag atcatggttc gctggtatcg ctgtcgaact tctccaccgt
     2221 cggtagcctg atgggtaacc tgacgcgcgg ctcaatgatg attgaaggac gaattgcgcg
     2281 ctttgtatat atctcgctat accgaatgca tcagattgcg ctgcatggtt actttaaaac
     2341 cggattaatg atgctggtgg ggagtattaa ccgcgttatc cgtccgcgtt tgaagttgca
     2401 tgcagcgaac gacgaaaatt acgcccttgc agcgtaagtc gacctgcagc caagcttggc
     2461 tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc
     2521 ggtctgataa aacagaattt gcctcccggc agtagcgcgg tggtcccacc tgaccccatg
     2581 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga
     2641 gtagccaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     2701 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga
     2761 tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc
     2821 caggcatcaa aggaatcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac
     2881 tctttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     2941 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     3001 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     3061 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     3121 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     3181 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     3241 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     3301 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     3361 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     3421 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     3481 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
     3541 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     3601 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     3661 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     3721 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     3781 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     3841 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     3901 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     3961 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4021 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4081 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4141 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4201 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4261 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4321 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4381 atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4441 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4501 cgcctggtat ctttatagtc ctgtcgggtt tcgccaacct ctgacttgag cgtcgatttt
     4561 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4621 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     4681 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     4741 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccttatcttt ccctttattt
     4801 ttgctgcggt aagtcgcata aaaaccattc ttcataattc aatccattta ctatgttatg
     4861 ttctgagggg agtgaaaatt cccctaattc gatgaagatt cttgctcaat tgttatcagc
     4921 tatgcgccga ccagaacacc ttgccgatca gccaaacgtc tcttcaggcc actgactagc
     4981 gataactttc cccacaacgg aacaactctc attgcatggg atcattgggt actgtgggtt
     5041 tagtggttgt aaaaacacct gaccgctatc cctgatcagt ttcttgaagg taaactcatc
     5101 acccccaagt ctggctatgc agaaatcacc tggctcaaca gcctgctcag ggtcaacgag
     5161 aattaacatt ccgtcaggaa agcttggctt ggagcctgtt ggtgcggtca tggaattacc
     5221 ttcaacctca agccagaatg cagaatcact ggcttttttg gttgtgctta cccatctctc
     5281 cgcatcacct ttggtaaagg ttctaagctt aggtgagaac atccctgcct gaacatgaga
     5341 aaaaacaggg tactcatact cacttctaag tgacggctgc atactaaccg cttcatacat
     5401 ctcgtagatt tctctggcga ttgaagggct aaattcttca acgctaactt tgagaatttt
     5461 tgcaagcaat gcggcgttat aagcatttaa tgcattgatg ccattaaata aagcaccaac
     5521 gcctgactgc cccatcccca tcttgtctgc gacagattcc tgggataagc caagttcatt
     5581 tttctttttt tcataaattg ctttaaggcg acgtgcgtcc tcaagctgct cttgtgttaa
     5641 tggtttcttt tttgtgctca t
//
