LOCUS       Exported                4057 bp DNA     circular SYN 03-JAN-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4057)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             64..456
                     /codon_start=1
                     /label=anti-CD47 nanobody
                     /translation="MAEVQLQASGGGLVQAGGSLRLSCAASGFTNERSQNMGWFRQAPG
                     KEREGVACISSRVVECWYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAAPRA
                     ITGTSFVGEHMCDYWGQGTQVTVSSGR"
     terminator      687..773
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      865..892
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        911..1002
                     /label=AmpR promoter
     CDS             1003..1860
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2034..2622
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2977..3687)
                     /codon_start=1
                     /label=lambda repressor (ts)
                     /note="temperature-sensitive variant of the phage lambda 
                     repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 gcagaaggct ttggggtgtg tgatacgaaa cgaagcattg gttaaaaatt aaggagggaa
       61 ttcatggccg aagttcagct gcaggccagc ggcggtggtc tggttcaggc cggtggtagc
      121 ctgcgtttaa gctgcgccgc cagcggtttc accaatgaac gcagccagaa catgggctgg
      181 tttcgccagg caccgggcaa agaacgcgaa ggcgtggcct gcattagcag ccgcgtggtt
      241 gaatgctggt atgccgatag cgtgaaaggc cgctttacca ttagccgcga taacgccaag
      301 aacaccgtgt acctgcagat gaacagcctg aaaccggagg ataccgccgt gtattattgc
      361 gcagcaccgc gcgccattac cggtaccagc ttcgtgggtg aacacatgtg cgattactgg
      421 ggtcagggta cccaggtgac cgtgagcagc ggtcgctagg atccgtcgac ctgcagccaa
      481 gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg
      541 cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga
      601 ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca
      661 tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      721 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg
      781 gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat
      841 aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc
      901 tacaaactct tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      961 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1021 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1081 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1141 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1201 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     1261 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1321 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1381 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1441 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     1501 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     1561 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     1621 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     1681 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     1741 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     1801 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     1861 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     1921 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     1981 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2041 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2101 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2161 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2221 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2281 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2341 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2401 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2461 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2521 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2581 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2641 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2701 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     2761 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgctt atctttccct
     2821 ttatttttgc tgcggtaagt cgcataaaaa ccattcttca taattcaatc catttactat
     2881 gttatgttct gaggggagtg aaaattcccc taattcgatg aagattcttg ctcaattgtt
     2941 atcagctatg cgccgaccag aacaccttgc cgatcagcca aacgtctctt caggccactg
     3001 actagcgata actttcccca caacggaaca actctcattg catgggatca ttgggtactg
     3061 tgggtttagt ggttgtaaaa acacctgacc gctatccctg atcagtttct tgaaggtaaa
     3121 ctcatcaccc ccaagtctgg ctatgcagaa atcacctggc tcaacagcct gctcagggtc
     3181 aacgagaatt aacattccgt caggaaagct tggcttggag cctgttggtg cggtcatgga
     3241 attaccttca acctcaagcc agaatgcaga atcactggct tttttggttg tgcttaccca
     3301 tctctccgca tcacctttgg taaaggttct aagcttaggt gagaacatcc ctgcctgaac
     3361 atgagaaaaa acagggtact catactcact tctaagtgac ggctgcatac taaccgcttc
     3421 atacatctcg tagatttctc tggcgattga agggctaaat tcttcaacgc taactttgag
     3481 aatttttgta agcaatgcgg cgttataagc atttaatgca ttgatgccat taaataaagc
     3541 accaacgcct gactgcccca tccccatctt gtctgcgaca gattcctggg ataagccaag
     3601 ttcatttttc tttttttcat aaattgcttt aaggcgacgt gcgtcctcaa gctgctcttg
     3661 tgttaatggt ttcttttttg tgctcatacg ttaaatctat caccgcaagg gataaatatc
     3721 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgca tgtactaagg
     3781 aggttgtatg gaacaacgca taaccctgaa agattatgca atgcgctttg ggcaaaccaa
     3841 gacagctaaa gatctctcac ctaccaaaca atgcccccct gcaaaaaata aattcatata
     3901 aaaaacatac agataaccat ctgcggtgat aaattatctc tggcggtgtt gacataaata
     3961 ccactggcgg tgatactgag cacatcagca ggacgcactg accaccatga aggtgacgct
     4021 cttaaaaatt aagccctgaa gaagggcagc attcaaa
//
