LOCUS       62056_4340        4819 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             64..780
                     /codon_start=1
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      1449..1535
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1627..1654
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1673..1764
                     /label=AmpR promoter
     CDS             1765..2622
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2796..3384
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3739..4449)
                     /codon_start=1
                     /label=lambda repressor (ts)
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 gcagaaggct ttggggtgtg tgatacgaaa cgaagcattg gttaaaaatt aaggagggaa
       61 ttcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      121 gacggcgacg taaacggcca caagttcagc gtgcgcggcg agggcgaggg cgatgccacc
      181 aacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
      241 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
      301 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
      361 agcttcaagg acgacggcac ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
      421 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
      481 cacaagctgg agtacaactt caacagccac aacgtctata tcaccgccga caagcagaag
      541 aacggcatca aggccaactt caagatccgc cacaacgtgg aggacggcag cgtgcagctc
      601 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
      661 cactacctga gcacccagtc cgtgctgagc aaagacccca acgagaagcg cgatcacatg
      721 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
      781 ggaggtggag gcagtggagg agggggaagc ggaggaggtg gaagcatggc cgaagttcag
      841 ctgcaggcca gcggcggtgg tctggttcag gccggtggta gcctgcgttt aagctgcgcc
      901 gccagcggtt tcaccaatga acgcagccag aacatgggct ggtttcgcca ggcaccgggc
      961 aaagaacgcg aaggcgtggc ctgcattagc agccgcgtgg ttgaatgctg gtatgccgat
     1021 agcgtgaaag gccgctttac cattagccgc gataacgcca agaacaccgt gtacctgcag
     1081 atgaacagcc tgaaaccgga ggataccgcc gtgtattatt gcgcagcacc gcgcgccatt
     1141 accggtacca gcttcgtggg tgaacacatg tgcgattact ggggtcaggg tacccaggtg
     1201 accgtgagca gcggtcgcta ggatccgtcg acctgcagcc aagcttggct gttttggcgg
     1261 atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa
     1321 acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga
     1381 agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg
     1441 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1501 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     1561 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa
     1621 ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttttgttta
     1681 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1741 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1801 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1861 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     1921 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     1981 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     2041 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     2101 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     2161 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     2221 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     2281 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     2341 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2401 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2461 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2521 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2581 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2641 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2701 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2761 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2821 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2881 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2941 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3001 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3061 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3121 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3181 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3241 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3301 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3361 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3421 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3481 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3541 gagtcagtga gcgaggaagc ggaagagcgc ttatctttcc ctttattttt gctgcggtaa
     3601 gtcgcataaa aaccattctt cataattcaa tccatttact atgttatgtt ctgaggggag
     3661 tgaaaattcc cctaattcga tgaagattct tgctcaattg ttatcagcta tgcgccgacc
     3721 agaacacctt gccgatcagc caaacgtctc ttcaggccac tgactagcga taactttccc
     3781 cacaacggaa caactctcat tgcatgggat cattgggtac tgtgggttta gtggttgtaa
     3841 aaacacctga ccgctatccc tgatcagttt cttgaaggta aactcatcac ccccaagtct
     3901 ggctatgcag aaatcacctg gctcaacagc ctgctcaggg tcaacgagaa ttaacattcc
     3961 gtcaggaaag cttggcttgg agcctgttgg tgcggtcatg gaattacctt caacctcaag
     4021 ccagaatgca gaatcactgg cttttttggt tgtgcttacc catctctccg catcaccttt
     4081 ggtaaaggtt ctaagcttag gtgagaacat ccctgcctga acatgagaaa aaacagggta
     4141 ctcatactca cttctaagtg acggctgcat actaaccgct tcatacatct cgtagatttc
     4201 tctggcgatt gaagggctaa attcttcaac gctaactttg agaatttttg taagcaatgc
     4261 ggcgttataa gcatttaatg cattgatgcc attaaataaa gcaccaacgc ctgactgccc
     4321 catccccatc ttgtctgcga cagattcctg ggataagcca agttcatttt tctttttttc
     4381 ataaattgct ttaaggcgac gtgcgtcctc aagctgctct tgtgttaatg gtttcttttt
     4441 tgtgctcata cgttaaatct atcaccgcaa gggataaata tctaacaccg tgcgtgttga
     4501 ctattttacc tctggcggtg ataatggttg catgtactaa ggaggttgta tggaacaacg
     4561 cataaccctg aaagattatg caatgcgctt tgggcaaacc aagacagcta aagatctctc
     4621 acctaccaaa caatgccccc ctgcaaaaaa taaattcata taaaaaacat acagataacc
     4681 atctgcggtg ataaattatc tctggcggtg ttgacataaa taccactggc ggtgatactg
     4741 agcacatcag caggacgcac tgaccaccat gaaggtgacg ctcttaaaaa ttaagccctg
     4801 aagaagggca gcattcaaa
//
