LOCUS       V013159                 4664 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013159
VERSION     V013159
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..33
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     sig_peptide     79..141
                     /label="OmpA signal peptide"
                     /note="signal peptide from the E. coli outer membrane
                     protein OmpA"
     CDS             142..1005
                     /gene="Pdcd1"
                     /label="Programmed cell death protein 1"
                     /note="Programmed cell death protein 1 from Mus musculus.
                     Accession#: Q02242"
     terminator      1237..1323
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1415..1442
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1461..1552
                     /label="AmpR promoter"
     CDS             1553..2410
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2584..3172
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3527..4237)
                     /label="lambda repressor (ts)"
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
ORIGIN
        1 gaattcatgt ttcacacagg aaacagctat gaccatgatt acggattcac tggaactcta
       61 gataacgagg cgcaaaaaat gaaaaagaca gctatcgcga ttgcagtggc actggcaggt
      121 ttcgctaccg tcgctcaggc tatgtgggtt cgtcaggttc cgtggtcttt cacctgggct
      181 gttctgcagc tgtcttggca gtctggttgg ctgctggaag ttccgaacgg tccgtggcgt
      241 tctctgacct tctacccggc ttggctgacc gtttctgaag gtgctaacgc taccttcacc
      301 tgctctctgt ctaactggtc tgaagacctg atgctgaact ggaaccgtct gtctccgtct
      361 aaccagaccg aaaaacaggc tgctttctgc aacggtctgt ctcagccggt tcaggacgct
      421 cgtttccaga tcatccagct gccgaaccgt cacgacttcc acatgaacat cctggacacc
      481 cgtcgtaacg actctggtat ctacctgtgc ggtgctatct ctctgcaccc gaaagctaaa
      541 atcgaagaat ctccgggtgc tgaactggtt gttaccgaac gtatcctgga aacctctacc
      601 cgttacccgt ctccgtctcc gaaaccggaa ggtcgtttcc agggtatggt tatcggtatc
      661 atgtctgctc tggttggtat cccggttctg ctgctgctgg cttgggctct ggctgttttc
      721 tgctctacct ctatgtctga agctcgtggt gctggttcta aagacgacac cctgaaagaa
      781 gaaccgtctg ctgctccggt tccgtctgtt gcttacgaag aactggactt ccagggtcgt
      841 gaaaaaaccc cggaactgcc gaccgcttgc gttcacaccg aatacgctac catcgttttc
      901 accgaaggtc tgggtgcttc tgctatgggt cgtcgtggtt ctgctgacgg tctgcagggt
      961 ccgcgtccgc cgcgtcacga agacggtcac tgctcttggc cgctgtaagg atccgtcgac
     1021 ctgcagccaa gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta
     1081 aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg
     1141 tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg
     1201 ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg
     1261 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     1321 aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga
     1381 cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt
     1441 tttgcgtttc tacaaactct tttgtttatt tttctaaata cattcaaata tgtatccgct
     1501 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     1561 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     1621 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     1681 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     1741 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     1801 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     1861 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     1921 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     1981 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2041 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2101 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     2161 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2221 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2281 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2341 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2401 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2461 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2521 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2581 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2641 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     2701 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     2761 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     2821 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2881 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2941 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3001 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3061 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3121 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3181 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3241 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3301 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgctt
     3361 atctttccct ttatttttgc tgcggtaagt cgcataaaaa ccattcttca taattcaatc
     3421 catttactat gttatgttct gaggggagtg aaaattcccc taattcgatg aagattcttg
     3481 ctcaattgtt atcagctatg cgccgaccag aacaccttgc cgatcagcca aacgtctctt
     3541 caggccactg actagcgata actttcccca caacggaaca actctcattg catgggatca
     3601 ttgggtactg tgggtttagt ggttgtaaaa acacctgacc gctatccctg atcagtttct
     3661 tgaaggtaaa ctcatcaccc ccaagtctgg ctatgcagaa atcacctggc tcaacagcct
     3721 gctcagggtc aacgagaatt aacattccgt caggaaagct tggcttggag cctgttggtg
     3781 cggtcatgga attaccttca acctcaagcc agaatgcaga atcactggct tttttggttg
     3841 tgcttaccca tctctccgca tcacctttgg taaaggttct aagcttaggt gagaacatcc
     3901 ctgcctgaac atgagaaaaa acagggtact catactcact tctaagtgac ggctgcatac
     3961 taaccgcttc atacatctcg tagatttctc tggcgattga agggctaaat tcttcaacgc
     4021 taactttgag aatttttgta agcaatgcgg cgttataagc atttaatgca ttgatgccat
     4081 taaataaagc accaacgcct gactgcccca tccccatctt gtctgcgaca gattcctggg
     4141 ataagccaag ttcatttttc tttttttcat aaattgcttt aaggcgacgt gcgtcctcaa
     4201 gctgctcttg tgttaatggt ttcttttttg tgctcatacg ttaaatctat caccgcaagg
     4261 gataaatatc taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgca
     4321 tgtactaagg aggttgtatg gaacaacgca taaccctgaa agattatgca atgcgctttg
     4381 ggcaaaccaa gacagctaaa gatctctcac ctaccaaaca atgcccccct gcaaaaaata
     4441 aattcatata aaaaacatac agataaccat ctgcggtgat aaattatctc tggcggtgtt
     4501 gacataaata ccactggcgg tgatactgag cacatcagca ggacgcactg accaccatga
     4561 aggtgacgct cttaaaaatt aagccctgaa gaagggcagc attcaaagca gaaggctttg
     4621 gggtgtgtga tacgaaacga agcattggtt aaaaattaag gagg
//
