LOCUS       V014351                 5224 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014351
VERSION     V014351
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     396..412
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     sig_peptide     458..520
                     /label="OmpA signal peptide"
                     /note="signal peptide from the E. coli outer membrane
                     protein OmpA"
     CDS             1082..1945
                     /gene="Pdcd1"
                     /label="Programmed cell death protein 1"
                     /note="Programmed cell death protein 1 from Mus musculus.
                     Accession#: Q02242"
     terminator      2177..2263
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2355..2382
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2401..2492
                     /label="AmpR promoter"
     CDS             2493..3350
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3524..4112
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4467..5177)
                     /label="lambda repressor (ts)"
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
ORIGIN
        1 ttgactattt tacctctggc ggtgataatg gttgcatgta ctaaggaggt tgtatggaac
       61 aacgcataac cctgaaagat tatgcaatgc gctttgggca aaccaagaca gctaaagatc
      121 tctcacctac caaacaatgc ccccctgcaa aaaataaatt catataaaaa acatacagat
      181 aaccatctgc ggtgataaat tatctctggc ggtgttgaca taaataccac tggcggtgat
      241 actgagcaca tcagcaggac gcactgacca ccatgaaggt gacgctctta aaaattaagc
      301 cctgaagaag ggcagcattc aaagcagaag gctttggggt gtgtgatacg aaacgaagca
      361 ttggttaaaa attaaggagg aattcatgtt tcacacagga aacagctatg accatgatta
      421 cggattcact ggaactctag ataacgaggc gcaaaaaatg aaaaagacag ctatcgcgat
      481 tgcagtggca ctggcaggtt tcgctaccgt cgctcaggct atgtatatga agagaatatt
      541 tatatatcta ttattacctt gtgcattcgc atgttctgct aatgataatg ttttttttgg
      601 caagggcaac aagcatcaga tctcttttgc tgcgggagaa agtataagaa gaggaggggt
      661 tgagcactta tatacggctt ttctgacata cagtgaaccc agcgattttt tctttttaca
      721 ggcaagaaat aatctggagt taggaggatt taaggctaag ggtagcgatg attgcagtaa
      781 acattctggc agcgttccct gtaataaata taaccagggc gtattgggta tctcgaagga
      841 tgtggcgctg gttcatttcg ctggtatcta taccggtatt ggtctggggg cttatataaa
      901 atctaagtcg cgagatgata tgcgtgtcaa ttctgcattt acctttggag aaaaagcgtt
      961 tcttggctgg aactttgggg ctttttctac agaagcttat atccggcatt tctcgaatgg
     1021 atcacttacg gataaaaatt cagggcataa ttttgtaggt gcttcaatta gttataattt
     1081 catgtgggtt cgtcaggttc cgtggtcttt cacctgggct gttctgcagc tgtcttggca
     1141 gtctggttgg ctgctggaag ttccgaacgg tccgtggcgt tctctgacct tctacccggc
     1201 ttggctgacc gtttctgaag gtgctaacgc taccttcacc tgctctctgt ctaactggtc
     1261 tgaagacctg atgctgaact ggaaccgtct gtctccgtct aaccagaccg aaaaacaggc
     1321 tgctttctgc aacggtctgt ctcagccggt tcaggacgct cgtttccaga tcatccagct
     1381 gccgaaccgt cacgacttcc acatgaacat cctggacacc cgtcgtaacg actctggtat
     1441 ctacctgtgc ggtgctatct ctctgcaccc gaaagctaaa atcgaagaat ctccgggtgc
     1501 tgaactggtt gttaccgaac gtatcctgga aacctctacc cgttacccgt ctccgtctcc
     1561 gaaaccggaa ggtcgtttcc agggtatggt tatcggtatc atgtctgctc tggttggtat
     1621 cccggttctg ctgctgctgg cttgggctct ggctgttttc tgctctacct ctatgtctga
     1681 agctcgtggt gctggttcta aagacgacac cctgaaagaa gaaccgtctg ctgctccggt
     1741 tccgtctgtt gcttacgaag aactggactt ccagggtcgt gaaaaaaccc cggaactgcc
     1801 gaccgcttgc gttcacaccg aatacgctac catcgttttc accgaaggtc tgggtgcttc
     1861 tgctatgggt cgtcgtggtt ctgctgacgg tctgcagggt ccgcgtccgc cgcgtcacga
     1921 agacggtcac tgctcttggc cgctgtaagg atccgtcgac ctgcagccaa gcttggctgt
     1981 tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt
     2041 ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg
     2101 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta
     2161 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     2221 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
     2281 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
     2341 gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct
     2401 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     2461 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     2521 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     2581 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     2641 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     2701 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     2761 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     2821 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     2881 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     2941 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3001 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     3061 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     3121 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     3181 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3241 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     3301 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     3361 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3421 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3481 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3541 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3601 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3661 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3721 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3781 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3841 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3901 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3961 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4021 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4081 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4141 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4201 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4261 agcgcagcga gtcagtgagc gaggaagcgg aagagcgctt atctttccct ttatttttgc
     4321 tgcggtaagt cgcataaaaa ccattcttca taattcaatc catttactat gttatgttct
     4381 gaggggagtg aaaattcccc taattcgatg aagattcttg ctcaattgtt atcagctatg
     4441 cgccgaccag aacaccttgc cgatcagcca aacgtctctt caggccactg actagcgata
     4501 actttcccca caacggaaca actctcattg catgggatca ttgggtactg tgggtttagt
     4561 ggttgtaaaa acacctgacc gctatccctg atcagtttct tgaaggtaaa ctcatcaccc
     4621 ccaagtctgg ctatgcagaa atcacctggc tcaacagcct gctcagggtc aacgagaatt
     4681 aacattccgt caggaaagct tggcttggag cctgttggtg cggtcatgga attaccttca
     4741 acctcaagcc agaatgcaga atcactggct tttttggttg tgcttaccca tctctccgca
     4801 tcacctttgg taaaggttct aagcttaggt gagaacatcc ctgcctgaac atgagaaaaa
     4861 acagggtact catactcact tctaagtgac ggctgcatac taaccgcttc atacatctcg
     4921 tagatttctc tggcgattga agggctaaat tcttcaacgc taactttgag aatttttgta
     4981 agcaatgcgg cgttataagc atttaatgca ttgatgccat taaataaagc accaacgcct
     5041 gactgcccca tccccatctt gtctgcgaca gattcctggg ataagccaag ttcatttttc
     5101 tttttttcat aaattgcttt aaggcgacgt gcgtcctcaa gctgctcttg tgttaatggt
     5161 ttcttttttg tgctcatacg ttaaatctat caccgcaagg gataaatatc taacaccgtg
     5221 cgtg
//
