LOCUS       62056_4300        4469 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             474..1190
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      1422..1508
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1600..1627
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1646..1737
                     /label=AmpR promoter
     CDS             1738..2595
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2769..3357
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3712..4422)
                     /codon_start=1
                     /label=lambda repressor (ts)
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 ttgactattt tacctctggc ggtgataatg gttgcatgta ctaaggaggt tgtatggaac
       61 aacgcataac cctgaaagat tatgcaatgc gctttgggca aaccaagaca gctaaagatc
      121 tctcacctac caaacaatgc ccccctgcaa aaaataaatt catataaaaa acatacagat
      181 aaccatctgc ggtgataaat tatctctggc ggtgttgaca taaataccac tggcggtgat
      241 actgagcaca tcagcaggac gcactgacca ccatgaaggt gacgctctta aaaattaagc
      301 cctgaagaag ggcagcattc aaagcagaag gctttggggt gtgtgatacg aaacgaagca
      361 ttggttaaaa attaaggagg gaattcatgg gtatcggtgc tgttctgaaa gttctgacca
      421 ccggtctgcc ggctctgatc tcttggatca aacgtaaacg tcagcagctc gagatggtga
      481 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      541 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      601 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      661 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
      721 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
      781 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      841 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
      901 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
      961 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     1021 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1081 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1141 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaggatccg
     1201 tcgacctgca gccaagcttg gctgttttgg cggatgagag aagattttca gcctgataca
     1261 gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc
     1321 ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag
     1381 tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc
     1441 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
     1501 ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg
     1561 caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg
     1621 gcctttttgc gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat
     1681 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1741 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1801 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1861 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1921 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1981 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2041 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2101 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2161 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2221 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2281 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2341 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2401 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     2461 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2521 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2581 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     2641 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     2701 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2761 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2821 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2881 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     2941 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3001 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3061 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3121 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3181 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3241 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3301 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3361 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3421 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3481 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3541 cgcttatctt tccctttatt tttgctgcgg taagtcgcat aaaaaccatt cttcataatt
     3601 caatccattt actatgttat gttctgaggg gagtgaaaat tcccctaatt cgatgaagat
     3661 tcttgctcaa ttgttatcag ctatgcgccg accagaacac cttgccgatc agccaaacgt
     3721 ctcttcaggc cactgactag cgataacttt ccccacaacg gaacaactct cattgcatgg
     3781 gatcattggg tactgtgggt ttagtggttg taaaaacacc tgaccgctat ccctgatcag
     3841 tttcttgaag gtaaactcat cacccccaag tctggctatg cagaaatcac ctggctcaac
     3901 agcctgctca gggtcaacga gaattaacat tccgtcagga aagcttggct tggagcctgt
     3961 tggtgcggtc atggaattac cttcaacctc aagccagaat gcagaatcac tggctttttt
     4021 ggttgtgctt acccatctct ccgcatcacc tttggtaaag gttctaagct taggtgagaa
     4081 catccctgcc tgaacatgag aaaaaacagg gtactcatac tcacttctaa gtgacggctg
     4141 catactaacc gcttcataca tctcgtagat ttctctggcg attgaagggc taaattcttc
     4201 aacgctaact ttgagaattt ttgtaagcaa tgcggcgtta taagcattta atgcattgat
     4261 gccattaaat aaagcaccaa cgcctgactg ccccatcccc atcttgtctg cgacagattc
     4321 ctgggataag ccaagttcat ttttcttttt ttcataaatt gctttaaggc gacgtgcgtc
     4381 ctcaagctgc tcttgtgtta atggtttctt ttttgtgctc atacgttaaa tctatcaccg
     4441 caagggataa atatctaaca ccgtgcgtg
//
