LOCUS       62056_4285        3773 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      482..568
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      660..687
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        706..797
                     /label=AmpR promoter
     CDS             798..1655
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1829..2417
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2772..3482)
                     /codon_start=1
                     /label=lambda repressor (ts)
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 agcacatcag caggacgcac tgaccaccat gaaggtgacg ctcttaaaaa ttaagccctg
       61 aagaagggca gcattcaaag cagaaggctt tggggtgtgt gatacgaaac gaagcattgg
      121 ttaaaaatta aggagggaat tcatgctgct gggcgatttt tttcgcaaaa gcaaagaaaa
      181 aattggcaaa gaatttaaac gcattgtgca gcgcattaaa gattttctgc gcaacctggt
      241 gccgcgcacc gaaagctaag tcgacctgca gccaagcttg gctgttttgg cggatgagag
      301 aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat
      361 ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa
      421 cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca
      481 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      541 ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca
      601 acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca
      661 gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttttgt ttatttttct
      721 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      781 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
      841 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
      901 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
      961 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     1021 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     1081 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     1141 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     1201 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1261 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     1321 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     1381 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     1441 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     1501 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     1561 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     1621 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     1681 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     1741 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1801 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1861 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1921 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     1981 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2041 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2101 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2161 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2221 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2281 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2341 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2401 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2461 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     2521 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     2581 tgagcgagga agcggaagag cgcttatctt tccctttatt tttgctgcgg taagtcgcat
     2641 aaaaaccatt cttcataatt caatccattt actatgttat gttctgaggg gagtgaaaat
     2701 tcccctaatt cgatgaagat tcttgctcaa ttgttatcag ctatgcgccg accagaacac
     2761 cttgccgatc agccaaacgt ctcttcaggc cactgactag cgataacttt ccccacaacg
     2821 gaacaactct cattgcatgg gatcattggg tactgtgggt ttagtggttg taaaaacacc
     2881 tgaccgctat ccctgatcag tttcttgaag gtaaactcat cacccccaag tctggctatg
     2941 cagaaatcac ctggctcaac agcctgctca gggtcaacga gaattaacat tccgtcagga
     3001 aagcttggct tggagcctgt tggtgcggtc atggaattac cttcaacctc aagccagaat
     3061 gcagaatcac tggctttttt ggttgtgctt acccatctct ccgcatcacc tttggtaaag
     3121 gttctaagct taggtgagaa catccctgcc tgaacatgag aaaaaacagg gtactcatac
     3181 tcacttctaa gtgacggctg catactaacc gcttcataca tctcgtagat ttctctggcg
     3241 attgaagggc taaattcttc aacgctaact ttgagaattt ttgtaagcaa tgcggcgtta
     3301 taagcattta atgcattgat gccattaaat aaagcaccaa cgcctgactg ccccatcccc
     3361 atcttgtctg cgacagattc ctgggataag ccaagttcat ttttcttttt ttcataaatt
     3421 gctttaaggc gacgtgcgtc ctcaagctgc tcttgtgtta atggtttctt ttttgtgctc
     3481 atacgttaaa tctatcaccg caagggataa atatctaaca ccgtgcgtgt tgactatttt
     3541 acctctggcg gtgataatgg ttgcatgtac taaggaggtt gtatggaaca acgcataacc
     3601 ctgaaagatt atgcaatgcg ctttgggcaa accaagacag ctaaagatct ctcacctacc
     3661 aaacaatgcc cccctgcaaa aaataaattc atataaaaaa catacagata accatctgcg
     3721 gtgataaatt atctctggcg gtgttgacat aaataccact ggcggtgata ctg
//
