LOCUS       62056_4280        4956 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     sig_peptide     317..382
                     /label=pelB signal sequence
                     /note="leader peptide for secretion"
     terminator      766..813
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        857..885
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             932..1639
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      1940..2026
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2118..2145
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2164..2255
                     /label=AmpR promoter
     CDS             2256..3113
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3287..3875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4230..4940)
                     /codon_start=1
                     /label=lambda repressor (ts)
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 cgcaagggat aaatatctaa caccgtgcgt gggatccaat aggggcaaac cgtgacgact
       61 tttttcatcg gtctcgctcc agttaatcaa atcacgcata cgcgctctcg actacagtat
      121 gcatctttta tgccacattt tatgtggggt cattccctga tattacgggc actatttatt
      181 caaaactctg acgaaaaaca ggctgtcgtc agttttgacg tgacgaaacg aaataccgcg
      241 tgacagccat cacgcggcag acattttatt ttttctcgac gacgggacct gcctgatacc
      301 cgcggccgct atacatatga aatacctgct gccgaccgct gctgctggtc tgctgctcct
      361 cgctgcccag ccggcgatgg cccaggttca gctggttgaa tctggtggtg gtctggttga
      421 accgggtggt tctctgcgtc tgtcttgcgc tgcttctggt atcatcttca aaatcaacga
      481 catgggttgg taccgtcagg ctccgggtaa acgtcgtgaa tgggttgctg cttctaccgg
      541 tggtgacgaa gctatctacc gtgactctgt taaagaccgt ttcaccatct ctcgtgacgc
      601 taaaaactct gttttcctgc agatgaactc tctgaaaccg gaagacaccg ctgtttacta
      661 ctgcaccgct gttatctcta ccgaccgtga cggtaccgaa tggcgtcgtt actggggtca
      721 gggtacccag gttaccgttt cttctggtgg ttagctgagc aataactagc ataacccctt
      781 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gacgctctcc cttatgcgac
      841 tcctgcatta ggaaatttga caattaatca tcggctcgta taatgtgtgg aatgtctcat
      901 gagcggatat ttcacacagg aaacagtatt catggtgagc aagggcgagg aggataacat
      961 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     1021 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     1081 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     1141 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     1201 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     1261 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     1321 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     1381 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     1441 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     1501 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     1561 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     1621 catggacgag ctgtacaagt aattaaccta ggctgctgcc accgctgagc aataactagc
     1681 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgcagc caagcttggc
     1741 tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc
     1801 ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1861 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga
     1921 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1981 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga
     2041 tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc
     2101 caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac
     2161 tcttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     2221 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     2281 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     2341 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     2401 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     2461 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     2521 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     2581 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     2641 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     2701 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     2761 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     2821 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     2881 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     2941 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     3001 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     3061 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     3121 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3181 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3241 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3301 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3361 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3421 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3481 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3541 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3601 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3661 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3721 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3781 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3841 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3901 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3961 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4021 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cttatctttc cctttatttt
     4081 tgctgcggta agtcgcataa aaaccattct tcataattca atccatttac tatgttatgt
     4141 tctgagggga gtgaaaattc ccctaattcg atgaagattc ttgctcaatt gttatcagct
     4201 atgcgccgac cagaacacct tgccgatcag ccaaacgtct cttcaggcca ctgactagcg
     4261 ataactttcc ccacaacgga acaactctca ttgcatggga tcattgggta ctgtgggttt
     4321 agtggttgta aaaacacctg accgctatcc ctgatcagtt tcttgaaggt aaactcatca
     4381 cccccaagtc tggctatgca gaaatcacct ggctcaacag cctgctcagg gtcaacgaga
     4441 attaacattc cgtcaggaaa gcttggcttg gagcctgttg gtgcggtcat ggaattacct
     4501 tcaacctcaa gccagaatgc agaatcactg gcttttttgg ttgtgcttac ccatctctcc
     4561 gcatcacctt tggtaaaggt tctaagctta ggtgagaaca tccctgcctg aacatgagaa
     4621 aaaacagggt actcatactc acttctaagt gacggctgca tactaaccgc ttcatacatc
     4681 tcgtagattt ctctggcgat tgaagggcta aattcttcaa cgctaacttt gagaattttt
     4741 gtaagcaatg cggcgttata agcatttaat gcattgatgc cattaaataa agcaccaacg
     4801 cctgactgcc ccatccccat cttgtctgcg acagattcct gggataagcc aagttcattt
     4861 ttcttttttt cataaattgc tttaaggcga cgtgcgtcct caagctgctc ttgtgttaat
     4921 ggtttctttt ttgtgctcat acgttaaatc tatcac
//
