LOCUS       62056_4275        4307 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             437..454
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      1305..1391
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1483..1510
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1528..1619
                     /label=AmpR promoter
     CDS             1620..2477
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2652..3240
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3597..4307)
                     /codon_start=1
                     /label=lambda repressor
                     /note="phage lambda repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 acgttaaatc tatcaccgca agggataaat atctaacacc gtgcgtgttg actattttac
       61 ctctggcggt gataatggtt gcatgtacta aggaggttgt atggaacaac gcataaccct
      121 gaaagattat gcaatgcgct ttgggcaaac caagacagct aaaagatctc tcacctacca
      181 aacaatgccc ccctgcaaaa aataaattca tataaaaaac atacagataa ccatctgcgg
      241 tgataaatta tctctggcgg tgttgacata aataccactg gcggtgatac tgagcacatc
      301 agcaggacgc actgaccacc atgaaggtga cgctcttaaa aattaagccc tgaagaaggg
      361 cagcattcaa agcagaaggc tttggggtgt gtgatacgaa acgaagcatt ggttaaaaat
      421 taaggaggaa ttcatgcatc atcatcatca tcatattgtc ggaggaagtg actggctggt
      481 gtccgccgca cactgcgtgt atgggagaaa cttagagcca tccaagtgga cagcaatcct
      541 aggcctgcat atgaaatcaa atctgacctc tcctcaaaca gtccctcgat caatagatga
      601 aattgtcata aaccctcatt acaataggcg aagaaaggac aacgacattg ccatgatgca
      661 tctggaattt aaagtgaatt acacagatta catacaacct atttgtttac cggaagaaaa
      721 tcaagttttt cctccaggaa gaaattgttc tattgctggt tgggggacgg ttgtatatca
      781 aggtactact gcaaacatat tgcaagaagc tgatgttcct cttctatcaa atgagagatg
      841 ccaacagcag atgccagaat ataacattac tgaaaatatg atatgtgcag gctatgaaga
      901 aggaggaata gattcttgtc agggggattc aggaggacca ttaatgtgcc aagaaaacaa
      961 caggtggttc cttgctggtg tgacctcatt tggatacaag tgtgccctgc ctaatcgccc
     1021 cggagtgtat gccagggtct caaggtttac cgagtggata caaagttttc tacattaggg
     1081 atccgtcgac ctgcagccaa gcttctgttt tggcttatga gagaagattt tcagcctgat
     1141 acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctc ccggcagtag
     1201 cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg
     1261 tagtgtgggg tctccccatg cgagagtagc caactgccag gcatcaaata aaacgaaagg
     1321 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     1381 gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc
     1441 gggcaggacg cccgccataa actgccaggc atcaaaggaa tcagaaggcc atcctgacgg
     1501 atggcctttt tgcgtttcta caaactcttt gtttattttt ctaaatacat tcaaatatgt
     1561 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     1621 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     1681 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     1741 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     1801 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     1861 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     1921 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     1981 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     2041 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     2101 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     2161 ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     2221 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     2281 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     2341 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     2401 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     2461 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     2521 taaaacttca tttttaattt aaaagggatc taggtgaaga tcctttttga taatctcatg
     2581 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2641 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2701 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2761 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     2821 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2881 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2941 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3001 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
     3061 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3121 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3181 caacctctga cttgagcgtc gattttgtga tgctcgtcag gggggcggag cctatggaaa
     3241 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3301 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3361 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3421 gagcgccctt atctttccct ttatttttgc tgcggtaagt cgcataaaaa ccattcttca
     3481 taattcaatc catttactat gttatgttct gaggggagtg aaaattcccc taattcgatg
     3541 aagattcttg ctcaattgtt atcagctatg cgccgaccag aacaccttgc cgatcagcca
     3601 aacgtctctt caggccactg actagcgata actttcccca caacggaaca actctcattg
     3661 catgggatca ttgggtactg tgggtttagt ggttgtaaaa acacctgacc gctatccctg
     3721 atcagtttct tgaaggtaaa ctcatcaccc ccaagtctgg ctatgcagaa atcacctggc
     3781 tcaacagcct gctcagggtc aacgagaatt aacattccgt caggaaagct tggcttggag
     3841 cctgttggtg cggtcatgga attaccttca acctcaagcc agaatgcaga atcactggct
     3901 tttttggttg tgcttaccca tctctccgca tcacctttgg taaaggttct aagcttaggt
     3961 gagaacatcc ctgcctgaac atgagaaaaa acagggtact catactcact tctaagtgac
     4021 ggctgcatac taaccgcttc atacatctcg tagatttctc tggcgattga agggctaaat
     4081 tcttcaacgc taactttgag aatttttgca agcaatgcgg cgttataagc atttaatgca
     4141 ttgatgccat taaataaagc accaacgcct gactgcccca tccccatctt gtctgcgaca
     4201 gattcctggg ataagccaag ttcatttttc tttttttcat aaattgcttt aaggcgacgt
     4261 gcgtcctcaa gctgctcttg tgttaatggt ttcttttttg tgctcat
//
