LOCUS       62056_4270        4382 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7..723
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      955..1041
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1133..1160
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1179..1270
                     /label=AmpR promoter
     CDS             1271..2128
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2302..2890
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3245..3955)
                     /codon_start=1
                     /label=lambda repressor (ts)
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 gaattcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
       61 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      121 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      181 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
      241 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      301 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      361 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      421 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
      481 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
      541 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      601 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
      661 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
      721 aagtaaggat ccgtcgacct gcagccaagc ttggctgttt tggcggatga gagaagattt
      781 tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg
      841 gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta
      901 gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata
      961 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1021 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc
     1081 ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc
     1141 atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt tctaaataca
     1201 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     1261 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     1321 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     1381 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     1441 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     1501 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     1561 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     1621 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     1681 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     1741 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     1801 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     1861 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     1921 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     1981 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     2041 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     2101 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     2161 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     2221 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     2281 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2341 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2401 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     2461 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2521 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2581 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2641 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2701 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2761 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2821 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2881 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2941 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3001 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     3061 ggaagcggaa gagcgcttat ctttcccttt atttttgctg cggtaagtcg cataaaaacc
     3121 attcttcata attcaatcca tttactatgt tatgttctga ggggagtgaa aattccccta
     3181 attcgatgaa gattcttgct caattgttat cagctatgcg ccgaccagaa caccttgccg
     3241 atcagccaaa cgtctcttca ggccactgac tagcgataac tttccccaca acggaacaac
     3301 tctcattgca tgggatcatt gggtactgtg ggtttagtgg ttgtaaaaac acctgaccgc
     3361 tatccctgat cagtttcttg aaggtaaact catcaccccc aagtctggct atgcagaaat
     3421 cacctggctc aacagcctgc tcagggtcaa cgagaattaa cattccgtca ggaaagcttg
     3481 gcttggagcc tgttggtgcg gtcatggaat taccttcaac ctcaagccag aatgcagaat
     3541 cactggcttt tttggttgtg cttacccatc tctccgcatc acctttggta aaggttctaa
     3601 gcttaggtga gaacatccct gcctgaacat gagaaaaaac agggtactca tactcacttc
     3661 taagtgacgg ctgcatacta accgcttcat acatctcgta gatttctctg gcgattgaag
     3721 ggctaaattc ttcaacgcta actttgagaa tttttgtaag caatgcggcg ttataagcat
     3781 ttaatgcatt gatgccatta aataaagcac caacgcctga ctgccccatc cccatcttgt
     3841 ctgcgacaga ttcctgggat aagccaagtt catttttctt tttttcataa attgctttaa
     3901 ggcgacgtgc gtcctcaagc tgctcttgtg ttaatggttt cttttttgtg ctcatacgtt
     3961 aaatctatca ccgcaaggga taaatatcta acaccgtgcg tgttgactat tttacctctg
     4021 gcggtgataa tggttgcatg tactaaggag gttgtatgga acaacgcata accctgaaag
     4081 attatgcaat gcgctttggg caaaccaaga cagctaaaga tctctcacct accaaacaat
     4141 gcccccctgc aaaaaataaa ttcatataaa aaacatacag ataaccatct gcggtgataa
     4201 attatctctg gcggtgttga cataaatacc actggcggtg atactgagca catcagcagg
     4261 acgcactgac caccatgaag gtgacgctct taaaaattaa gccctgaaga agggcagcat
     4321 tcaaagcaga aggctttggg gtgtgtgata cgaaacgaag cattggttaa aaattaagga
     4381 gg
//
