LOCUS       V013124                 5328 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013124
VERSION     V013124
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7..723
                     /label="EGFP"
                     /note="enhanced GFP"
     terminator      733..819
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      911..938
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             1319..1651
                     /gene="mazF"
                     /label="Endoribonuclease toxin MazF"
                     /note="Endoribonuclease toxin MazF from Escherichia coli
                     (strain K12). Accession#: P0AE70"
     CDS             1652..1675
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     terminator      complement(1904..1947)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        2125..2216
                     /label="AmpR promoter"
     CDS             2217..3074
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3248..3836
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4191..4901)
                     /label="lambda repressor (ts)"
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
ORIGIN
        1 gaattcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
       61 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      121 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      181 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
      241 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      301 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      361 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      421 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
      481 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
      541 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      601 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
      661 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
      721 aagtaaggat cccaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      781 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
      841 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
      901 caaattaagc agaaggccat cctgacggat ggcctttttt gactatttta cctctggcgg
      961 tgataatggt tgcatgtact aaggaggttg tatggaacaa cgcataaccc tgaaagatta
     1021 tgcaatgcgc tttgggcaaa ccaagacagc taaagatctc tcacctacca aacaatgccc
     1081 ccctgcaaaa aataaattca tataaaaaac atacagataa ccatctgcgg tgataaatta
     1141 tctctggcgg tgttgacata aataccactg gcggtgatac tgagcacatc agcaggacgc
     1201 actgaccacc atgaaggtga cgctcttaaa aattaagccc tgaagaaggg cagcattcaa
     1261 agcagaaggc tttggggtgt gtgatacgaa acgaagcatt ggttaaaaat taaggaggat
     1321 ggtaagccga tacgtacccg atatgggcga tctgatttgg gttgattttg acccgacaaa
     1381 aggtagcgag caagctggac atcgtccagc tgttgtcctg agtcctttca tgtacaacaa
     1441 caaaacaggt atgtgtctgt gtgttccttg tacaacgcaa tcaaaaggat atccgttcga
     1501 agttgtttta tccggtcagg aacgtgatgg cgtagcgtta gctgatcagg taaaaagtat
     1561 cgcctggcgg gcaagaggag caacgaagaa aggaacagtt gccccagagg aattacaact
     1621 cattaaagcc aaaattaacg tactgattgg ggactacaag gacgacgatg acaagtaggt
     1681 cgacctgcag ccaagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag
     1741 attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg
     1801 gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt
     1861 gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca
     1921 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
     1981 gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc
     2041 aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg
     2101 cctttttgcg tttctacaaa ctcttttgtt tatttttcta aatacattca aatatgtatc
     2161 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     2221 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     2281 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     2341 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     2401 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     2461 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     2521 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     2581 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     2641 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     2701 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     2761 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     2821 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     2881 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     2941 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     3001 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     3061 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     3121 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     3181 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3241 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3301 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3361 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3421 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3481 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3541 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3601 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3661 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3721 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3781 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3841 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3901 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3961 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     4021 gcttatcttt ccctttattt ttgctgcggt aagtcgcata aaaaccattc ttcataattc
     4081 aatccattta ctatgttatg ttctgagggg agtgaaaatt cccctaattc gatgaagatt
     4141 cttgctcaat tgttatcagc tatgcgccga ccagaacacc ttgccgatca gccaaacgtc
     4201 tcttcaggcc actgactagc gataactttc cccacaacgg aacaactctc attgcatggg
     4261 atcattgggt actgtgggtt tagtggttgt aaaaacacct gaccgctatc cctgatcagt
     4321 ttcttgaagg taaactcatc acccccaagt ctggctatgc agaaatcacc tggctcaaca
     4381 gcctgctcag ggtcaacgag aattaacatt ccgtcaggaa agcttggctt ggagcctgtt
     4441 ggtgcggtca tggaattacc ttcaacctca agccagaatg cagaatcact ggcttttttg
     4501 gttgtgctta cccatctctc cgcatcacct ttggtaaagg ttctaagctt aggtgagaac
     4561 atccctgcct gaacatgaga aaaaacaggg tactcatact cacttctaag tgacggctgc
     4621 atactaaccg cttcatacat ctcgtagatt tctctggcga ttgaagggct aaattcttca
     4681 acgctaactt tgagaatttt tgtaagcaat gcggcgttat aagcatttaa tgcattgatg
     4741 ccattaaata aagcaccaac gcctgactgc cccatcccca tcttgtctgc gacagattcc
     4801 tgggataagc caagttcatt tttctttttt tcataaattg ctttaaggcg acgtgcgtcc
     4861 tcaagctgct cttgtgttaa tggtttcttt tttgtgctca tacgttaaat ctatcaccgc
     4921 aagggataaa tatctaacac cgtgcgtgtt gactatttta cctctggcgg tgataatggt
     4981 tgcatgtact aaggaggttg tatggaacaa cgcataaccc tgaaagatta tgcaatgcgc
     5041 tttgggcaaa ccaagacagc taaagatctc tcacctacca aacaatgccc ccctgcaaaa
     5101 aataaattca tataaaaaac atacagataa ccatctgcgg tgataaatta tctctggcgg
     5161 tgttgacata aataccactg gcggtgatac tgagcacatc agcaggacgc actgaccacc
     5221 atgaaggtga cgctcttaaa aattaagccc tgaagaaggg cagcattcaa agcagaaggc
     5281 tttggggtgt gtgatacgaa acgaagcatt ggttaaaaat taaggagg
//
