LOCUS       62056_4260        3476 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      239..325
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      417..444
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        463..565
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             566..1222
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     rep_origin      1396..1984
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2339..3049)
                     /codon_start=1
                     /label=lambda repressor (ts)
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 gaattcccgg ggatccgtcg acctgcagcc aagcttggct gttttggcgg atgagagaag
       61 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg
      121 cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca
      241 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      301 gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa
      421 ggccatcctg acggatggcc tttttgcgtt tctacaaact cttgatcggc acgtaagagg
      481 ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg agttatcgag
      541 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
      601 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
      661 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
      721 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
      781 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
      841 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
      901 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
      961 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag
     1021 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
     1081 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
     1141 tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg
     1201 cgatgagtgg cagggcgggg cgtaactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ttatctttcc ctttattttt gctgcggtaa gtcgcataaa aaccattctt
     2221 cataattcaa tccatttact atgttatgtt ctgaggggag tgaaaattcc cctaattcga
     2281 tgaagattct tgctcaattg ttatcagcta tgcgccgacc agaacacctt gccgatcagc
     2341 caaacgtctc ttcaggccac tgactagcga taactttccc cacaacggaa caactctcat
     2401 tgcatgggat cattgggtac tgtgggttta gtggttgtaa aaacacctga ccgctatccc
     2461 tgatcagttt cttgaaggta aactcatcac ccccaagtct ggctatgcag aaatcacctg
     2521 gctcaacagc ctgctcaggg tcaacgagaa ttaacattcc gtcaggaaag cttggcttgg
     2581 agcctgttgg tgcggtcatg gaattacctt caacctcaag ccagaatgca gaatcactgg
     2641 cttttttggt tgtgcttacc catctctccg catcaccttt ggtaaaggtt ctaagcttag
     2701 gtgagaacat ccctgcctga acatgagaaa aaacagggta ctcatactca cttctaagtg
     2761 acggctgcat actaaccgct tcatacatct cgtagatttc tctggcgatt gaagggctaa
     2821 attcttcaac gctaactttg agaatttttg taagcaatgc ggcgttataa gcatttaatg
     2881 cattgatgcc attaaataaa gcaccaacgc ctgactgccc catccccatc ttgtctgcga
     2941 cagattcctg ggataagcca agttcatttt tctttttttc ataaattgct ttaaggcgac
     3001 gtgcgtcctc aagctgctct tgtgttaatg gtttcttttt tgtgctcata cgttaaatct
     3061 atcaccgcaa gggataaata tctaacaccg tgcgtgttga ctattttacc tctggcggtg
     3121 ataatggttg catgtactaa ggaggttgta tggaacaacg cataaccctg aaagattatg
     3181 caatgcgctt tgggcaaacc aagacagcta aagatctctc acctaccaaa caatgccccc
     3241 ctgcaaaaaa taaattcata taaaaaacat acagataacc atctgcggtg ataaattatc
     3301 tctggcggtg ttgacataaa taccactggc ggtgatactg agcacatcag caggacgcac
     3361 tgaccaccat gaaggtgacg ctcttaaaaa ttaagccctg aagaagggca gcattcaaag
     3421 cagaaggctt tggggtgtgt gatacgaaac gaagcattgg ttaaaaatta aggagg
//
