LOCUS       62056_4205        7624 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7624)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..44
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             664..1269
                     /codon_start=1
                     /label=LexA
                     /note="E. coli DNA-binding protein that represses genes
                     involved in the SOS response to DNA damage"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     CDS             1288..1521
                     /codon_start=1
                     /label=VP16 AD
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et 
                     al., 1989)"
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     terminator      1526..1772
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(1791..1809)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1819..1835)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1976..2431
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2731..3135
                     /label=LEU2 promoter
     CDS             3148..4239
                     /codon_start=1
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(4963..5775)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      6217..6805
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(6982..7483)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
ORIGIN
        1 ccgcggatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
       61 tcatttggcg agcgttggtt ggtggatcaa gcccacgcgt aggcaatcct cgagcagatc
      121 cgccaggcgt gtatatatag cgtggatggc caggcaactt tagtgctgac acatacaggc
      181 atatatatat gtgtgcgacg acacatgatc atatggcatg catgtgctct gtatgtatat
      241 aaaactcttg ttttcttctt ttctctaaat attctttcct tatacattag gacctttgca
      301 gcataaatta ctatacttct atagacacgc aaacacaaat acacacacta atctagatat
      361 atgatgcttt tgcaagcttt ccttttcctt ttggctggtt ttgcagccaa aatatctgca
      421 atggccatta cggccaggcc tttaattaag gccgcctcgg ccatctgcag gaattcgata
      481 tcaagcttat cgataccgtc gaccatgtcg ggggggatcc ctccagatca acaaagattg
      541 atctttgccg gtaagcagct agaagacggt agaacgctgt ctgattacaa cattcagaag
      601 gagtccacct tacatcttgt gctaaggcta agaggtggta tgcacagatc agcttgcggc
      661 cgcatgaaag cgttaacggc caggcaacaa gaggtgtttg atctcatccg tgatcacatc
      721 agccagacag gtatgccgcc gacgcgtgcg gaaatcgcgc agcgtttggg gttccgttcc
      781 ccaaacgcgg ctgaagaaca tctgaaggcg ctggcacgca aaggcgttat tgaaattgtt
      841 tccggcgcat cacgcgggat ccgtctgttg caggaagagg aagaagggtt gccgctggta
      901 ggtcgtgtgg ctgccggtga accacttctg gcgcaacagc atattgaagg tcattatcag
      961 gtcgaccctt ccttattcaa gccgaatgct gatttcctgc tgcgcgtcag cgggatgtcg
     1021 atgaaagata tcggcattat ggatggtgac ttgctggcag tgcataaaac tcaggatgta
     1081 cgtaacggtc aggtcgttgt cgcacgtatt gatgacgagg ttaccgttaa gcgcctgaaa
     1141 aaacagggca ataaagtcga actgttgcca gaaaatagcg agtttaaacc aattgtcgta
     1201 gatcttcgtc agcagagctt caccattgaa gggctggcgg ttggggttat tcgcaacggc
     1261 gactggctgg aattcccggg gatctgggcc cccccgaccg atgtcagcct gggggacgag
     1321 ctccacttag acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat
     1381 ctggacatgt tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc
     1441 ccctacggcg ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt
     1501 ggaattgacg agtacggtgg gtaggtcatg taattagtta tgtcacgctt acattcacgc
     1561 cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
     1621 cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt
     1681 ctttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga
     1741 aggttttggg acgctcgaag gctttaattt gcggccggta cccaattcgc cctatagtga
     1801 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     1861 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     1921 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg
     1981 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     2041 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     2101 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     2161 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     2221 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     2281 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     2341 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     2401 aattttaaca aaatattaac gtttacaatt tcctgatgcg gtattttctc cttacgcatc
     2461 tgtgcggtat ttcacaccgc atatcgacgg tcgaggagaa cttctagtat atccacatac
     2521 ctaatattat tgccttatta aaaatggaat cccaacaatt acatcaaaat ccacattctc
     2581 ttcaaaatca attgtcctgt acttccttgt tcatgtgtgt tcaaaaacgt tatatttata
     2641 ggataattat actctatttc tcaacaagta attggttgtt tggccgagcg gtctaaggcg
     2701 cctgattcaa gaaatatctt gaccgcagtt aactgtggga atactcaggt atcgtaagat
     2761 gcaagagttc gaatctctta gcaaccatta tttttttcct caacataacg agaacacaca
     2821 ggggcgctat cgcacagaat caaattcgat gactggaaat tttttgttaa tttcagaggt
     2881 cgcctgacgc atataccttt ttcaactgaa aaattgggag aaaaaggaaa ggtgagaggc
     2941 cggaaccggc ttttcatata gaatagagaa gcgttcatga ctaaatgctt gcatcacaat
     3001 acttgaagtt gacaatatta tttaaggacc tattgttttt tccaataggt ggttagcaat
     3061 cgtcttactt tctaactttt cttacctttt acatttcagc aatatatata tatatttcaa
     3121 ggatatacca ttctaatgtc tgcccctatg tctgccccta agaagatcgt cgttttgcca
     3181 ggtgaccacg ttggtcaaga aatcacagcc gaagccatta aggttcttaa agctatttct
     3241 gatgttcgtt ccaatgtcaa gttcgatttc gaaaatcatt taattggtgg tgctgctatc
     3301 gatgctacag gtgtcccact tccagatgag gcgctggaag cctccaagaa ggttgatgcc
     3361 gttttgttag gtgctgtggg tggtcctaaa tggggtaccg gtagtgttag acctgaacaa
     3421 ggtttactaa aaatccgtaa agaacttcaa ttgtacgcca acttaagacc atgtaacttt
     3481 gcatccgact ctcttttaga cttatctcca atcaagccac aatttgctaa aggtactgac
     3541 ttcgttgttg tcagagaatt agtgggaggt atttactttg gtaagagaaa ggaagacgat
     3601 ggtgatggtg tcgcttggga tagtgaacaa tacaccgttc cagaagtgca aagaatcaca
     3661 agaatggccg ctttcatggc cctacaacat gagccaccat tgcctatttg gtccttggat
     3721 aaagctaatg ttttggcctc ttcaagatta tggagaaaaa ctgtggagga aaccatcaag
     3781 aacgaattcc ctacattgaa ggttcaacat caattgattg attctgccgc catgatccta
     3841 gttaagaacc caacccacct aaatggtatt ataatcacca gcaacatgtt tggtgatatc
     3901 atctccgatg aagcctccgt tatcccaggt tccttgggtt tgttgccatc tgcgtccttg
     3961 gcctctttgc cagacaagaa caccgcattt ggtttgtacg aaccatgcca cggttctgct
     4021 ccagatttgc caaagaataa ggttgaccct atcgccacta tcttgtctgc tgcaatgatg
     4081 ttgaaattgt cattgaactt gcctgaagaa ggtaaggcca ttgaagatgc agttaaaaag
     4141 gttttggatg caggtatcag aactggtgat ttaggtggtt ccaacagtac caccgaagtc
     4201 ggtgatgctg tcgccgaaga agttaagaaa atccttgctt aaaaagattc tcttttttta
     4261 tgatatttgt acataaactt tataaatgaa attcataata gaaacgacac gaaattacaa
     4321 aatggaatat gttcataggg tagacgaaac tatatacgca atctacatac atttatcaag
     4381 aaggagaaaa aggaggatag taaaggaata caggtaagca aattgatact aatggctcaa
     4441 cgtgataagg aaaaagaatt gcactttaac attaatattg acaaggagga gggcaccaca
     4501 caaaaagtta ggtgtaacag aaaatcatga aactacgatt cctaatttga tattggagga
     4561 ttttctctaa aaaaaaaaaa atacaacaaa taaaaaacac tcaatgacct gaccatttga
     4621 tggagtttaa gtcaatacct tcttgaacca tttcccataa tggtgaaagt tccctcaaga
     4681 attttactct gtcagaaacg gccttacgac gtagtcgata tggtgcactc tcagtacaat
     4741 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     4801 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggac
     4861 cacatgtgga tgatccagtt cgatttattc aacaaagccg ccgtcccgtc aagtcagcgt
     4921 aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact catcgagcat
     4981 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
     5041 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
     5101 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     5161 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     5221 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     5281 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     5341 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     5401 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     5461 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     5521 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     5581 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     5641 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     5701 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctcgagcaag acgtttcccg
     5761 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     5821 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     5881 gcttactagg atcgatccga catgtggcgg tatcattgca gcactggggc cagatggtaa
     5941 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     6001 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     6061 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6121 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6181 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6241 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6301 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6361 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6421 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6481 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6541 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6601 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6661 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6721 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6781 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6841 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6901 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     6961 cgagtcagtg agcgaggaag ctcgcttgcc tgtaacttac acgcgcctcg tatcttttaa
     7021 tgatggaata atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat
     7081 tttagaaagt aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa
     7141 ggtttaaaaa atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc
     7201 agattaaata gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg
     7261 tgtgtggtct tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa
     7321 gagcaagata aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa
     7381 aacaaaaact attttttctt taatttcttt ttttactttc tatttttaat ttatatattt
     7441 atattaaaaa atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg
     7501 cacttttcgg ggaaatgtgc gcggaacccc taagcggaag agcgcccaat acgcaaaccg
     7561 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7621 aaag
//
