LOCUS       V012975                 7073 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V012975
VERSION     V012975
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2492..3139
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_feature    complement(3955..4011)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     terminator      4111..4197
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        4323..4427
                     /label="AmpR promoter"
     CDS             4428..5285
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5459..6047
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(6233..6373)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 tattaacttt cggttgcaaa gctctaggat ttttaatgga cgcagcgcat cacacgcaaa
       61 aaggaaattg gaataaatgc gaaatttgag atgttaatta aagacctttt tgaggtcttt
      121 ttttcttaga tttttggggt tatttagggg agaaaacata ggggggtact acgacctccc
      181 ccctaggtgt ccattgtcca ttgtccaaac aaataaataa atattgggtt tttaatgtta
      241 aaaggttgtt ttttatgtta aagtgaaaaa aacagatgtt gggaggtaca gtgatggttg
      301 tagatagaaa agaagagaaa aaagttgctg ttactttaag acttacaaca gaagaaaatg
      361 agatattaaa tagaatcaaa gaaaaatata atattagcaa atcagatgca accggtattc
      421 taataaaaaa atatgcaaag gaggaatacg gtgcatttta aacaaaaaaa gatagacagc
      481 actggcatgc tgcctatcta tgactaaatt ttgttaaatg tattagcacc gttattatat
      541 catgagcgaa aatgtaataa aagaaactga aaacaagaaa aattcaagag gacgtaattg
      601 gacatttgtt ttatatccag aatcagcaaa agccgagtgg ttagagtatt taaaagagtt
      661 acacattcaa tttgtagtgt ctccattaca tgatagggat actgatacag aagataggat
      721 gaaaaaagag cattatcata ttctagtgat gtatgagggt aataaatctt atgaacagat
      781 aaaaataatt acagaagaat tgaatgcgac tattccgcag attgcaggaa gtgtgaaagg
      841 tcttgtgaga tatatgcttc acatggacga tcctaataaa tttaaatatc aaaaagaaga
      901 tatgatagtt tatggcggtg tagatgttga tgaattatta aagaaaacaa caacagatag
      961 atataaatta attaaagaaa tgattgagtt tattgatgaa caaggaatcg tagaatttaa
     1021 gagtttaatg gattatgcaa tgaagtttaa atttgatgat tggttcccgc ttttatgtga
     1081 taactcggcg tatgttattc aagaatatat aaaatcaaat cggtataaat ctgaccgata
     1141 gattttgaat ttaagagtgt cacaagacac tcttttttcg caccagcgaa aactggttta
     1201 agccgactgc gcaaaagaca taatcgattc acaaaaaata ggcacacgaa aaacaagtta
     1261 agggatgcag tttatgcatc ccttaactta cttattaaat aatttatagc tattgaaaag
     1321 agataagaat tgttcaaagc taatattgtt taaatcgtca attcctgcat gttttaagga
     1381 attgttaaat tgattttttg taaatatttt cttgtattct ttgttaaccc atttcataac
     1441 gaaataatta tacttttgtt tatctttgtg tgatattctt gatttttttc tacttaatct
     1501 gataagtgag ctattcactt taggtttagg atgaaaatat tctcttggaa ccatacttaa
     1561 tatagaaata tcaacttctg ccattaaaag taatgccaat gagcgttttg tatttaataa
     1621 tcttttagca aacccgtatt ccacgattaa ataaatctca ttagctatac tatcaaaaac
     1681 aattttgcgt attatatccg tacttatgtt ataaggtata ttaccatata ttttatagga
     1741 ttggttttta ggaaatttaa actgcaatat atccttgttt aaaacttgga aattatcgtg
     1801 atcttccttc aggttatgac catctgtgcc agttcgtaat gtctggtcaa ctttccgact
     1861 ctgagaaact tctggaatcg ctagagaatt tctggaatgg gattcaggag tggacagaac
     1921 gacacggata tatagtggat gtgtcaaaac gcataccatt ttgaacgatg acctctaata
     1981 attgttaatc atgttggtta cgtatttatt aacttctcct agtattagta attatcatgg
     2041 ctgtcatggc gcattaacgg aataaagggt gtgcttaaat cgggccattt tgcgtaataa
     2101 gaaaaaggat taattatgag cgaattgaat taataataag gtaatagatt tacattagaa
     2161 aatgaaaggg gattttatgc gtgagaatgt tacagtctat cccggcaata gttaccctta
     2221 ttatcaagat aagaaagaaa aggatttttc gctacgctca aatcctttaa aaaaacacaa
     2281 aagaccacat tttttaatgt ggtcttttat tcttcaacta aagcacccat tagttcaaca
     2341 aacgaaaatt ggataaagtg ggatattttt aaaatatata tatttatgtt acagtaatat
     2401 tgacttttaa aaaaggattg attctaatga agaaagcaga caagtaagcc tcctaaattc
     2461 actttagata aaaatttagg aggcatatca aatgaacttt aataaaattg atttagacaa
     2521 ttggaagaga aaagagatat ttaatcatta tttgaaccaa caaacgactt ttagtataac
     2581 cacagaaatt gatattagtg ttttataccg aaacataaaa caagaaggat ataaatttta
     2641 ccctgcattt attttcttag tgacaagggt gataaactca aatacagctt ttagaactgg
     2701 ttacaatagc gacggagagt taggttattg ggataagtta gagccacttt atacaatttt
     2761 tgatggtgta tctaaaacat tctctggtat ttggactcct gtaaagaatg acttcaaaga
     2821 gttttatgat ttataccttt ctgatgtaga gaaatataat ggttcgggga aattgtttcc
     2881 caaaacacct atacctgaaa atgctttttc tctttctatt attccatgga cttcatttac
     2941 tgggtttaac ttaaatatca ataataatag taattacctt ctacccatta ttacagcagg
     3001 aaaattcatt aataaaggta attcaatata tttaccgcta tctttacagg tacatcattc
     3061 tgtttgtgat ggttatcatg caggattgtt tatgaactct attcaggaat tgtcagatag
     3121 gcctaatgac tggcttttat aatatgagat aatgccgact gtacttttta cagtcggttt
     3181 tctaacgata cattaatagg tacgaaaaag caactttttt tgcgcttaaa accagtcata
     3241 ccaataactt aagggtaact agcctcgccg gaaagagcga aaatgcctca catttgtgcc
     3301 acctaaaaag gagcgattta catatgagtt atgcagtttg tagaatgcaa aaagtgaaat
     3361 cagctggact aaaaggcatg caatttcata atcaaagaga gcgaaaaagt agaacgaatg
     3421 atgatattga ccatgagcga acacgtgaaa attatgattt gaaaaatgat aaaaatattg
     3481 attacaacga acgtgtcaaa gaaattattg aatcacaaaa aacaggtaca agaaaaacga
     3541 ggaaagatgc tgttcttgta aatgagttgc tagtaacatc tgaccgagat ttttttgagc
     3601 aactggatcg atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc
     3661 aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac
     3721 gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc
     3781 gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc
     3841 tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt
     3901 catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt
     3961 gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt ccgctgcact
     4021 gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc
     4081 ctggggtaat gactctctag cttgaggcat caaataaaac gaaaggctca gtcgaaagac
     4141 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     4201 ccgctctagc tagaattaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
     4261 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     4321 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4381 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4441 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4501 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4561 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4621 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     4681 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     4741 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     4801 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     4861 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     4921 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     4981 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5041 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5101 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5161 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5221 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5281 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5341 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5401 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5461 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5521 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5581 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     5641 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5701 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5761 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     5821 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     5881 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     5941 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6001 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6061 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6121 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6181 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     6241 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     6301 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     6361 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     6421 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     6481 gttttcaccg tcatcaccga aacgcgcgag gcaggaacgg attgttgatg attacgaaaa
     6541 tattaagagc acagactatt acacagaaaa tcaagaatta aaaaaacgta gagagagttt
     6601 gaaagaagta gtgaatacat ggaaagaggg gtatcacgaa aaaagtaaag aggttaataa
     6661 attaaagcga gagaatgata gtttgaatga gcagttgaat gtatcagaga aatttcaaga
     6721 tagtacagtg actttatatc gtgctgcgag ggcgaatttc cctgggtttg agaaagggtt
     6781 taataggctt aaagagaaat tctttaatga ttccaaattc gagcgtgtgg gacagtttat
     6841 ggatgttgta caggataatg tccagaaggt cgatagaaag cgtgagaaac agcgtacaga
     6901 cgatttagag atgtagaggt acttttatgc cgagaaaact ttttgcgtgt gacagtcctt
     6961 aaaatatact tagagcgtaa gcgaaagtag tagcgacagc tattaacttt cggttgcaaa
     7021 gctctaggat ttttaatgga cgcagcgcat cacacgcaaa aaggaaattg gaa
//
