LOCUS       40924_7456        3242 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBSk-MP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Chakrabarty PK, Kalbande BB, Chavhan RL, Warade JG, Narwade AV, 
            Sable SV, Monga D, Mayee CD.
  TITLE     Inverted repeat constructs for RNA interference-mediated targeting 
            of movement/pre-coat protein genes of Cotton leaf curl virus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Chakrabarty PK, Kalbande BB, Chavhan RL, Warade JG, Narwade AV, 
            Sable SV, Monga D, Mayee CD.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2010) Division of Crop Improvement, Central 
            Institute for Cotton Research, Post Bag No. 02, Shankar Nagar P.O., 
            Nagpur, Maharashtra 440010, India
REFERENCE   3  (bases 1 to 3242)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3242)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-DEC-2010) Division of Crop Improvement, Central Institute for 
            Cotton Research, Post Bag No. 02, Shankar Nagar P.O., Nagpur, 
            Maharashtra 440010, India"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    653..1041
                     /note="MCS; multiple cloning site"
     misc_feature    659..767
                     /note="Cotton leaf curl virus isolate Sirsa (Haryana) 
                     movement/pre-coat protein fragment"
     intron          774..896
                     /note="chitinase intron 1; derived from Gossypium hirsutum"
     misc_feature    complement(903..1011)
                     /note="Cotton leaf curl virus isolate Sirsa (Haryana) 
                     movement/pre-coat protein fragment"
     promoter        complement(1054..1072)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1093..1109)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1117..1133)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1141..1171)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1186..1207)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1495..2083)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2257..3114)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3115..3219)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacctg
      661 atacggttca cgggtttcgg tgtatgcttt ctgtgaaata tttgcaactt ttgtcgcagg
      721 attattcacc ggatacgctt gggtacgagt taatacggga tttaattgaa ttccctgcag
      781 caatgcatca atcatgaaat ttaattgtta aaacgataat attttctaag attgtttgca
      841 aaagtcatgt tgaaatgatg ataaacattt tgtatctagt ttcgaaatgg actaacggat
      901 ccaattaaat cccgtattaa ctcgtaccca agcgtatccg gtgaataatc ctgcgacaaa
      961 agttgcaaat atttcacaga aagcatacac cgaaacccgt gaaccgtatc atctagagcg
     1021 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     1081 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     1141 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     1201 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     1261 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     1321 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     1381 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     1441 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     1501 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1561 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1621 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1681 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1741 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1801 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1861 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1921 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1981 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2041 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2101 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2161 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2221 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     2281 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     2341 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     2401 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     2461 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     2521 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     2581 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     2641 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     2701 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     2761 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     2821 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2881 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     2941 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3001 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3061 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3121 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3181 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3241 ac
//
