LOCUS       40924_7436        3394 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBSk-CP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Chakrabarty PK, Kalbande BB, Chavhan RL, Warade JG, Narwade AV, 
            Sable SV, Monga D, Mayee CD.
  TITLE     Inverted repeat constructs for RNA interference-mediated targeting 
            of movement/pre-coat protein genes of Cotton leaf curl virus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Chakrabarty PK, Kalbande BB, Chavhan RL, Warade JG, Narwade AV, 
            Sable SV, Monga D, Mayee CD.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2010) Division of Crop Improvement, Central 
            Institute for Cotton Research, Post Bag No. 02, Shankar Nagar P.O., 
            Nagpur, Maharashtra 440010, India
REFERENCE   3  (bases 1 to 3394)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3394)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-DEC-2010) Division of Crop Improvement, Central Institute for 
            Cotton Research, Post Bag No. 02, Shankar Nagar P.O., Nagpur, 
            Maharashtra 440010, India"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    659..1193
                     /note="MCS; multiple cloning site"
     misc_feature    659..843
                     /note="Cotton leaf curl virus isolate CLCuV coat protein 
                     fragment"
     intron          850..972
                     /note="chitinase intron 1; derived from Gossypium hirsutum"
     misc_feature    complement(979..1163)
                     /note="Cotton leaf curl virus isolate CLCuV coat protein 
                     fragment"
     promoter        complement(1206..1224)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1245..1261)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1269..1285)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1293..1323)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1338..1359)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1647..2235)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2409..3266)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3267..3371)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccat
      661 gtcgaagcga gctgccgata tcgtcatttc tacgcccgcg tcgaaagtac gccggcgtct
      721 gaacttcggc agcccataca ccagccgtgc tgctgccccc attgtccgcg tcacaaaaca
      781 acaggcatgg acaaacaggc ctatgaacag gaagcccagg atgtacagga tgtacagaag
      841 tccgaattcc ctgcagcaat gcatcaatca tgaaatttaa ttgttaaaac gataatattt
      901 tctaagattg tttgcaaaag tcatgttgaa atgatgataa acattttgta tctagtttcg
      961 aaatggacta acggatccgg acttctgtac atcctgtaca tcctgggctt cctgttcata
     1021 ggcctgtttg tccatgcctg ttgttttgtg acgcggacaa tgggggcagc agcacggctg
     1081 gtgtatgggc tgccgaagtt cagacgccgg cgtactttcg acgcgggcgt agaaatgacg
     1141 atatcggcag ctcgcttcga cattctagag cggccgccac cgcggtggag ctccagcttt
     1201 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     1261 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     1321 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     1381 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     1441 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1501 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1561 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1621 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1681 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1741 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1801 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1861 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1921 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1981 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2041 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     2101 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2161 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2221 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2281 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2341 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     2401 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     2461 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     2521 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     2581 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     2641 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     2701 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     2761 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     2821 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     2881 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     2941 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3001 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3061 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3121 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3181 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3241 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3301 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3361 ggggttccgc gcacatttcc ccgaaaagtg ccac
//
