LOCUS       40924_7431        3378 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBSk-BV4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Chakrabarty PK, Kalbande BB, Chavhan RL, Warade JG, Narwade AV, 
            Sable SV, Monga D, Mayee CD.
  TITLE     Inverted repeat constructs for RNA interference-mediated targeting 
            of movement/pre-coat protein genes of Cotton leaf curl virus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Chakrabarty PK, Kalbande BB, Chavhan RL, Warade JG, Narwade AV, 
            Sable SV, Monga D, Mayee CD.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2010) Division of Crop Improvement, Central 
            Institute for Cotton Research, Post Bag No. 02, Shankar Nagar P.O., 
            Nagpur, Maharashtra 440010, India
REFERENCE   3  (bases 1 to 3378)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3378)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-DEC-2010) Division of Crop Improvement, Central Institute for 
            Cotton Research, Post Bag No. 02, Shankar Nagar P.O., Nagpur, 
            Maharashtra 440010, India"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    653..1177
                     /note="MCS; multiple cloning site"
     misc_feature    659..835
                     /note="Cotton leaf curl virus isolate Sirsa (Haryana)
                     beta-V4 protein fragment"
     intron          842..964
                     /note="chitinase intron 1; derived from Gossypium hirsutum"
     misc_feature    complement(971..1147)
                     /note="Cotton leaf curl virus isolate Sirsa (Haryana)
                     beta-V4 protein fragment"
     promoter        complement(1190..1208)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1229..1245)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1253..1269)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1277..1307)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1322..1343)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1631..2219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2393..3250)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3251..3355)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgc
      661 ttgaaatctt tatacatggg tttgtaccgt atatacctat atatacggat agaaaacgga
      721 tgagaaaagg aaaaacggaa ctgaagagga aaaacaaaga aaagaaacaa ggatatatta
      781 tttatgaaag aaatgggagc gcagcgaatc gaaacaggaa aacccaagga aagaggaatt
      841 ccctgcagca atgcatcaat catgaaattt aattgttaaa acgataatat tttctaagat
      901 tgtttgcaaa agtcatgttg aaatgatgat aaacattttg tatctagttt cgaaatggac
      961 taacggatcc ctctttcctt gggttttcct gtttcgattc gctgcgctcc catttctttc
     1021 ataaataata tatccttgtt tcttttcttt gtttttcctc ttcagttccg tttttccttt
     1081 tctcatccgt tttctatccg tatatatagg tatatacggt acaaacccat gtataaagat
     1141 ttcaagctct agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag
     1201 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     1261 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     1321 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     1381 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     1441 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1501 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1561 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1621 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1681 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1741 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1801 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1861 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1921 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1981 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2041 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     2101 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2161 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2221 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2281 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2341 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2401 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2461 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2521 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2581 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2641 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2701 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     2761 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     2821 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     2881 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2941 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3001 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3061 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3121 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3181 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3241 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3301 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3361 ttccccgaaa agtgccac
//
