LOCUS       40924_7426        3448 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBSk-BC4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Chakrabarty PK, Kalbande BB, Chavhan RL, Warade JG, Narwade AV, 
            Sable SV, Monga D, Mayee CD.
  TITLE     Inverted repeat constructs for RNA interference-mediated targeting 
            of movement/pre-coat protein genes of Cotton leaf curl virus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Chakrabarty PK, Kalbande BB, Chavhan RL, Warade JG, Narwade AV, 
            Sable SV, Monga D, Mayee CD.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2010) Division of Crop Improvement, Central 
            Institute for Cotton Research, Post Bag No. 02, Shankar Nagar P.O., 
            Nagpur, Maharashtra 440010, India
REFERENCE   3  (bases 1 to 3448)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3448)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-DEC-2010) Division of Crop Improvement, Central Institute for 
            Cotton Research, Post Bag No. 02, Shankar Nagar P.O., Nagpur, 
            Maharashtra 440010, India"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    653..1247
                     /note="MCS; multiple cloning site"
     misc_feature    659..870
                     /note="Cotton leaf curl virus isolate CLCuV coat protein
                     beta-C4 protein fragment"
     intron          877..999
                     /note="chitinase intron 1; derived from Gossypium hirsutum"
     misc_feature    complement(1006..1217)
                     /note="Cotton leaf curl virus isolate Sirsa (Haryana)
                     beta-C4 protein fragment"
     promoter        complement(1260..1278)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1299..1315)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1323..1339)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1347..1377)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1392..1413)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1701..2289)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2463..3320)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3321..3425)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccat
      661 ccattcccaa tatctctggg ttttcaagta caagtatatc aagtctgtga actatatctt
      721 ctatctcgat ctcttctatc tttgccccgt tgtatgcgaa taggaaattc gctatgatgc
      781 tcccttcaaa gccgttgaag tcgaatggaa cggtgtatgt cttcgtacgt gtactggacg
      841 atcccttcat acttgattag cgatggtgac gaattccctg cagcaatgca tcaatcatga
      901 aatttaattg ttaaaacgat aatattttct aagattgttt gcaaaagtca tgttgaaatg
      961 atgataaaca ttttgtatct agtttcgaaa tggactaacg gatccgtcac catcgctaat
     1021 caagtatgaa gggatcgtcc agtacacgta cgaagacata caccgttcca ttcgacttca
     1081 acggctttga agggagcatc atagcgaatt tcctattcgc atacaacggg gcaaagatag
     1141 aagagatcga gatagaagat atagttcaca gacttgatat acttgtactt gaaaacccag
     1201 agatattggg aatggattct agagcggccg ccaccgcggt ggagctccag cttttgttcc
     1261 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     1321 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     1381 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     1441 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     1501 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1561 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1621 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1681 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1741 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1801 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1861 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1921 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1981 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2041 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2101 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     2161 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2221 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2281 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2341 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     2401 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     2461 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     2521 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     2581 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     2641 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     2701 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     2761 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     2821 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     2881 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     2941 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3001 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3061 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3121 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     3181 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     3241 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     3301 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     3361 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     3421 ccgcgcacat ttccccgaaa agtgccac
//
