LOCUS       40924_7401        4102 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBSd191R, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Radeck J, Meyer D, Lautenschlager N, Mascher T.
  TITLE     Bacillus SEVA siblings: A Golden Gate-based toolbox to create 
            personalized integrative vectors for Bacillus subtilis
  JOURNAL   Sci Rep 7 (1), 14134 (2017)
  PUBMED    29074996
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Radeck J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2017) Biology, Technische Universitat Dresden, 
            Zellescher Weg 20b, Dresden 01627, Germany
REFERENCE   3  (bases 1 to 4102)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4102)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2017"; volume: "7"; issue: "1"; pages: "14134"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-APR-2017) Biology, Technische Universitat Dresden, Zellescher 
            Weg 20b, Dresden 01627, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             217..891
                     /codon_start=1
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      1050..1144
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1270..1361
                     /label=AmpR promoter
     CDS             1362..2219
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2226..2265
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2412..2520
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2555..3143
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3329..3469)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(3574..3762)
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(4010..4096)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt accttgtcgg ataaagctgt gttatattat gtcttggtgt taaatacaca
      121 cgcttaacga tttatgcaga gggtgctgca ggcggcagtt ctgtacaaaa atgacctaag
      181 cggaggaaaa aaaccattat attaggagga aataacatgg cctcttcaga ggatgttatt
      241 aaagaattta tgcggtttaa ggtgaggatg gaaggctcgg tgaacggaca tgagttcgaa
      301 attgagggag aaggtgaagg ccgcccttat gaaggtactc agacagcgaa attgaaagtc
      361 acgaaaggcg gaccgctgcc gtttgcttgg gacattctct cacctcaatt tcaatatggc
      421 tcaaaagcct acgtaaaaca cccggctgac atccctgatt acttaaagct atccttcccg
      481 gagggcttta aatgggaacg agttatgaat tttgaggacg gcggcgtcgt tactgtcaca
      541 caggattctt cccttcagga tggcgaattt atttacaaag taaaacttcg tggaactaac
      601 ttcccaagtg atggtcccgt gatgcaaaaa aaaacaatgg gatgggaagc atctacggaa
      661 cgtatgtatc cggaggatgg agccttaaag ggtgaaatca aaatgcgcct gaaacttaaa
      721 gatggcggac actatgacgc ggaagttaaa acaacatata tggctaaaaa accagtccaa
      781 ctgccgggag catataagac ggatataaag ttggacatta ccagccataa tgaagattac
      841 acgattgtgg aacagtatga gagagcagag ggcagacata gcacaggcgc gtaagaatta
      901 atgaaaaata agcggcagcc tgcttttcca tgcgggctgc cgcttatcgg gttattgcgg
      961 ccgcgtttat aatgaagtct agagtcgacc tgcaggcatg caagcttgcg gccgcgtcgt
     1021 gactgggaaa accctggcga ctagtcttgg actcctgttg atagatccag taatgacctc
     1081 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga
     1141 gaatccaggg gtccccaata attacgattt gcgatgaatg gtctcactca gaagacttct
     1201 cacctgctcg tctcactcaa tttaaattag tagcccgcct aatgagcggg ctttttttta
     1261 attcccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1321 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagcatt cagcattttc
     1381 gtgtggcgct gattccgttt tttgcggcgt tttgcctgcc ggtgtttgcg catccggaaa
     1441 ccctggtgaa agtgaaagat gcggaagatc aactgggtgc gcgcgtgggc tatattgaac
     1501 tggatctgaa cagcggcaaa attctggaat cttttcgtcc ggaagaacgt tttccgatga
     1561 tgagcacctt taaagtgctg ctgtgcggtg cggttctgag ccgtgtggat gcgggccagg
     1621 aacaactggg ccgtcgtatt cattatagcc agaacgatct ggtggaatat agcccggtga
     1681 ccgaaaaaca tctgaccgat ggcatgaccg tgcgtgaact gtgcagcgcg gcgattacca
     1741 tgagcgataa caccgcggcg aacctgctgc tgacgaccat tggcggtccg aaagaactga
     1801 ccgcgtttct gcataacatg ggcgatcatg tgacccgtct ggatcgttgg gaaccggaac
     1861 tgaacgaagc gattccgaac gatgaacgtg ataccaccat gccggcagca atggcgacca
     1921 ccctgcgtaa actgctgacg ggtgagctgc tgaccctggc aagccgccag caactgattg
     1981 attggatgga agcggataaa gtggcgggtc cgctgctgcg tagcgcgctg ccggctggct
     2041 ggtttattgc ggataaaagc ggtgcgggcg aacgtggcag ccgtggcatt attgcggcgc
     2101 tgggcccgga tggtaaaccg agccgtattg tggtgattta taccaccggc agccaggcga
     2161 cgatggatga acgtaaccgt cagattgcgg aaattggcgc gagcctgatt aaacattggt
     2221 aaaccgatac aattaaaggc tccttttgga gccttttttt ttggacgacc cttgtccttt
     2281 tccgctgcat aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt
     2341 ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa
     2401 cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc
     2461 actgtccctt attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg
     2521 taggccggcc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2581 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2641 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2701 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2761 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2821 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2881 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2941 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3001 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3061 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3121 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3181 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3241 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3301 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     3361 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     3421 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     3481 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     3541 agctgtgacg ctctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     3601 cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc
     3661 ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat
     3721 aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg
     3781 gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg
     3841 ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt
     3901 atggatgcgg cgggatattg gcgcgccacc ctgagaccca cccgcaggtg ttacatcgca
     3961 cccaagtctt caccctgaga cgttcgcgcc cagctgtcta gggcggcgga tttgtcctac
     4021 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     4081 cctttcgttt tatttgatgc ct
//
