LOCUS       40924_7396        4682 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBSd141R, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Radeck J, Meyer D, Lautenschlager N, Mascher T.
  TITLE     Bacillus SEVA siblings: A Golden Gate-based toolbox to create 
            personalized integrative vectors for Bacillus subtilis
  JOURNAL   Sci Rep 7 (1), 14134 (2017)
  PUBMED    29074996
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Radeck J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2017) Biology, Technische Universitat Dresden, 
            Zellescher Weg 20b, Dresden 01627, Germany
REFERENCE   3  (bases 1 to 4682)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4682)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2017"; volume: "7"; issue: "1"; pages: "14134"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-APR-2017) Biology, Technische Universitat Dresden, Zellescher 
            Weg 20b, Dresden 01627, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             217..891
                     /codon_start=1
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      1050..1144
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1270..1361
                     /label=AmpR promoter
     CDS             1362..2219
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2226..2265
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2412..2520
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2614..3202
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3259..4089)
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      4103..4454
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(4590..4676)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt accttgtcgg ataaagctgt gttatattat gtcttggtgt taaatacaca
      121 cgcttaacga tttatgcaga gggtgctgca ggcggcagtt ctgtacaaaa atgacctaag
      181 cggaggaaaa aaaccattat attaggagga aataacatgg cctcttcaga ggatgttatt
      241 aaagaattta tgcggtttaa ggtgaggatg gaaggctcgg tgaacggaca tgagttcgaa
      301 attgagggag aaggtgaagg ccgcccttat gaaggtactc agacagcgaa attgaaagtc
      361 acgaaaggcg gaccgctgcc gtttgcttgg gacattctct cacctcaatt tcaatatggc
      421 tcaaaagcct acgtaaaaca cccggctgac atccctgatt acttaaagct atccttcccg
      481 gagggcttta aatgggaacg agttatgaat tttgaggacg gcggcgtcgt tactgtcaca
      541 caggattctt cccttcagga tggcgaattt atttacaaag taaaacttcg tggaactaac
      601 ttcccaagtg atggtcccgt gatgcaaaaa aaaacaatgg gatgggaagc atctacggaa
      661 cgtatgtatc cggaggatgg agccttaaag ggtgaaatca aaatgcgcct gaaacttaaa
      721 gatggcggac actatgacgc ggaagttaaa acaacatata tggctaaaaa accagtccaa
      781 ctgccgggag catataagac ggatataaag ttggacatta ccagccataa tgaagattac
      841 acgattgtgg aacagtatga gagagcagag ggcagacata gcacaggcgc gtaagaatta
      901 atgaaaaata agcggcagcc tgcttttcca tgcgggctgc cgcttatcgg gttattgcgg
      961 ccgcgtttat aatgaagtct agagtcgacc tgcaggcatg caagcttgcg gccgcgtcgt
     1021 gactgggaaa accctggcga ctagtcttgg actcctgttg atagatccag taatgacctc
     1081 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga
     1141 gaatccaggg gtccccaata attacgattt gcgatgaatg gtctcactca gaagacttct
     1201 cacctgctcg tctcactcaa tttaaattag tagcccgcct aatgagcggg ctttttttta
     1261 attcccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1321 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagcatt cagcattttc
     1381 gtgtggcgct gattccgttt tttgcggcgt tttgcctgcc ggtgtttgcg catccggaaa
     1441 ccctggtgaa agtgaaagat gcggaagatc aactgggtgc gcgcgtgggc tatattgaac
     1501 tggatctgaa cagcggcaaa attctggaat cttttcgtcc ggaagaacgt tttccgatga
     1561 tgagcacctt taaagtgctg ctgtgcggtg cggttctgag ccgtgtggat gcgggccagg
     1621 aacaactggg ccgtcgtatt cattatagcc agaacgatct ggtggaatat agcccggtga
     1681 ccgaaaaaca tctgaccgat ggcatgaccg tgcgtgaact gtgcagcgcg gcgattacca
     1741 tgagcgataa caccgcggcg aacctgctgc tgacgaccat tggcggtccg aaagaactga
     1801 ccgcgtttct gcataacatg ggcgatcatg tgacccgtct ggatcgttgg gaaccggaac
     1861 tgaacgaagc gattccgaac gatgaacgtg ataccaccat gccggcagca atggcgacca
     1921 ccctgcgtaa actgctgacg ggtgagctgc tgaccctggc aagccgccag caactgattg
     1981 attggatgga agcggataaa gtggcgggtc cgctgctgcg tagcgcgctg ccggctggct
     2041 ggtttattgc ggataaaagc ggtgcgggcg aacgtggcag ccgtggcatt attgcggcgc
     2101 tgggcccgga tggtaaaccg agccgtattg tggtgattta taccaccggc agccaggcga
     2161 cgatggatga acgtaaccgt cagattgcgg aaattggcgc gagcctgatt aaacattggt
     2221 aaaccgatac aattaaaggc tccttttgga gccttttttt ttggacgacc cttgtccttt
     2281 tccgctgcat aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt
     2341 ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa
     2401 cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc
     2461 actgtccctt attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg
     2521 taggccggcc gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     2581 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2641 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2701 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2761 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2821 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2881 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2941 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3001 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggc atccggtaag
     3061 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3121 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3181 aggggggcgg agcctatgga aaaacgccag caacgcggcc gtgaaaggca ggccggtccg
     3241 tggtggccac ggcctctagg ccagatccag cggcatctgg gttagtcgag cgcgggccgc
     3301 ttcccatgtc tcaccagggc gagcctgttt cgcgatctca gcatctgaaa tcttcccggc
     3361 cttgcgcttc gctggggcct tacccaccgc cttggcgggc ttcttcggtc caaaactgaa
     3421 caacagatgt gtgaccttgc gcccggtctt tcgctgcgcc cactccacct gtagcgggct
     3481 gtgctcgttg atctgcgtca cggctggatc aagcactcgc aacttgaagt ccttgatcga
     3541 gggataccgg ccttccagtt gaaaccactt tcgcagctgg tcaatttcta tttcgcgctg
     3601 gccgatgctg tcccattgca tgagcagctc gtaaagcctg atcgcgtggg tgctgtccat
     3661 cttggccacg tcagccaagg cgtatttggt gaactgtttg gtgagttccg tcaggtacgg
     3721 cagcatgtct ttggtgaacc tgagttctac acggccctca ccctcccggt agatgattgt
     3781 ttgcacccag ccggtaatca tcacactcgg tcttttcccc ttgccattgg gctcttgggt
     3841 taaccggact tcccgccgtt tcaggcgcag ggccgcttct ttgagctggt tgtaggaaga
     3901 ttcgataggg acacccgcca tcgtcgctat gtcctccgcc gtcactgaat acatcacttc
     3961 atcggtgaca ggctcgctcc tcttcacctg gctaatacag gccagaacga tccgctgttc
     4021 ctgaacactg aggcgatacg cggcctcgac cagggcattg cttttgtaaa ccattggggg
     4081 tgaggccacg ttcgacattc cttgtgtata aggggacact gtatctgcgt cccacaatac
     4141 aacaaatccg tccctttaca acaacaaatc cgtcccttct taacaacaaa tccgtccctt
     4201 aatggcaaca aatccgtccc tttttaaact ctacaggcca cggattacgt ggcctgtaga
     4261 cgtcctaaaa ggtttaaaag ggaaaaggaa gaaaagggtg gaaacgcaaa aaacgcacca
     4321 ctacgtggcc ccgttggggc cgcatttgtg cccctgaagg ggcgggggag gcgtctgggc
     4381 aatccccgtt ttaccagtcc cctatcgccg cctgagaggg cgcaggaagc gagtaatcag
     4441 ggtatcgagg cggattcacc cttggcgtcc aaccagcggc accagcggcg cctgagaggg
     4501 gcgcgccacc ctgagaccca cccgcaggtg ttacatcgca cccaagtctt caccctgaga
     4561 cgttcgcgcc cagctgtcta gggcggcgga tttgtcctac tcaggagagc gttcaccgac
     4621 aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc
     4681 ct
//
