LOCUS       40924_7386        4412 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBSc393K, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Radeck J, Meyer D, Lautenschlager N, Mascher T.
  TITLE     Bacillus SEVA siblings: A Golden Gate-based toolbox to create 
            personalized integrative vectors for Bacillus subtilis
  JOURNAL   Sci Rep 7 (1), 14134 (2017)
  PUBMED    29074996
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Radeck J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2017) Biology, Technische Universitat Dresden, 
            Zellescher Weg 20b, Dresden 01627, Germany
REFERENCE   3  (bases 1 to 4412)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4412)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2017"; volume: "7"; issue: "1"; pages: "14134"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-APR-2017) Biology, Technische Universitat Dresden, Zellescher 
            Weg 20b, Dresden 01627, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    125..146
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        161..191
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    199..215
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     223..239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    249..305
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(309..325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      604..698
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        793..895
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             896..1552
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     oriT            1715..1823
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      1858..2446
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2632..2772)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2877..3065)
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3347..3374)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(3389..4180)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     terminator      complement(4320..4406)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
       61 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      241 atgattacga attcgagctc ggtacccggg gatcctctag agtcgacctg caggcatgca
      301 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      361 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      421 accgatcgcc cttcctaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      481 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      541 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgaactagtc
      601 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
      661 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aggggtcccc aataattacg
      721 atttgagatg agaccttgca ggtgagatga gacggtcttc aacatcgcag taaattggcg
      781 aaaatgacac gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc
      841 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga
      901 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
      961 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
     1021 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
     1081 tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct
     1141 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
     1201 ttcatccctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
     1261 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
     1321 gtttttcgta tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
     1381 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
     1441 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
     1501 cagaatgctt aatgaattac aacagtactg tgatgagtgg cagggcgggg cgtaatttga
     1561 cttcggtccg cgcgttgtcc ttttccgctg cataaccctg cttcggggtc attatagcga
     1621 ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg
     1681 tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac
     1741 ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga
     1801 atcctgctct gcgaggctgg ccgtaggccg gcccgtagaa aagatcaaag gatcttcttg
     1861 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1921 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1981 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     2041 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2101 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2161 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2221 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2281 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2341 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2401 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2461 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2521 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2581 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     2641 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     2701 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     2761 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     2821 ctcccggcat ccgcttacag acaagctgtg acgctctccg ggagctgcat gtgtcagagg
     2881 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg
     2941 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga
     3001 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
     3061 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa
     3121 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt
     3181 tgtgagggta aacaactggc ggtatggatg cggcgggata ttggcgcgtt cacctgcgat
     3241 ggcgaagact tgcgaggtct cagcgatcgt ctcagcgagg cgcgtaaaat aataaaaaag
     3301 ccggattaat aatctggctt tttatattct ctctctagta tataaacgca gaaaggccca
     3361 cccgaaggtg agccagtgtg aggtactaaa acaattcatc cagtaaaata taatatttta
     3421 ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt
     3481 ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc
     3541 tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc
     3601 tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa
     3661 aatcatacag ctcgcgcgga tctttaaatg gagtgtcctc ttcccagttt tcgcaatcca
     3721 catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat
     3781 tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca
     3841 gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat
     3901 cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg
     3961 gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg
     4021 tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca
     4081 gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga
     4141 tcagtttttt caattccggt gatattctca ttttagccat ttattatttc cttcctcttt
     4201 tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact
     4261 gttccttgca ttctaaaacc ttaaatacca gaaacgcgcc cagctgtcta gggcggcgga
     4321 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
     4381 ttcgactgag cctttcgttt tatttgatgc ct
//
