LOCUS       40924_7376        3963 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBSc391K, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Radeck J, Meyer D, Lautenschlager N, Mascher T.
  TITLE     Bacillus SEVA siblings: A Golden Gate-based toolbox to create 
            personalized integrative vectors for Bacillus subtilis
  JOURNAL   Sci Rep 7 (1), 14134 (2017)
  PUBMED    29074996
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Radeck J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2017) Biology, Technische Universitat Dresden, 
            Zellescher Weg 20b, Dresden 01627, Germany
REFERENCE   3  (bases 1 to 3963)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3963)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2017"; volume: "7"; issue: "1"; pages: "14134"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-APR-2017) Biology, Technische Universitat Dresden, Zellescher 
            Weg 20b, Dresden 01627, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      155..249
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        344..446
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             447..1103
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     oriT            1266..1374
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      1409..1997
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2183..2323)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2428..2616)
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(2898..2925)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(2940..3731)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     terminator      complement(3871..3957)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttgagat gagaccttgc aggtgagatg
      301 agacggtctt caacatcgca gtaaattggc gaaaatgaca cgttgatcgg cacgtaagag
      361 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga
      421 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
      481 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
      541 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
      601 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
      661 aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
      721 acaccgtttt ccatgagcaa actgaaacgt tttcatccct ctggagtgaa taccacgacg
      781 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
      841 cctatttccc taaagggttt attgagaata tgtttttcgt atcagccaat ccctgggtga
      901 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
      961 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
     1021 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
     1081 gtgatgagtg gcagggcggg gcgtaatttg acttcggtcc gcgcgttgtc cttttccgct
     1141 gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca
     1201 cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt
     1261 cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc
     1321 ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccgtaggcc
     1381 ggcccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1441 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1501 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     1561 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1621 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1681 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1741 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1801 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1861 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     1921 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     1981 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2041 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     2101 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     2161 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     2221 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     2281 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
     2341 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     2401 gacgctctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     2461 gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc
     2521 atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg
     2581 ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt
     2641 tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac
     2701 tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat
     2761 gcggcgggat attggcgcgt tcacctgcga tggcgaagac ttgcgaggtc tcagcgatcg
     2821 tctcagcgag gcgcgtaaaa taataaaaaa gccggattaa taatctggct ttttatattc
     2881 tctctctagt atataaacgc agaaaggccc acccgaaggt gagccagtgt gaggtactaa
     2941 aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt
     3001 aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg
     3061 cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca
     3121 cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca
     3181 agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat
     3241 ggagtgtcct cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa
     3301 tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga tatgtcgatg
     3361 gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg gctttgttca
     3421 tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag
     3481 ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc
     3541 atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt
     3601 tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg agacattcct
     3661 tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg tgatattctc
     3721 attttagcca tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag
     3781 ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc
     3841 agaaacgcgc ccagctgtct agggcggcgg atttgtccta ctcaggagag cgttcaccga
     3901 caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg
     3961 cct
//
