LOCUS       40924_7371        4556 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBSc293K, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Radeck J, Meyer D, Lautenschlager N, Mascher T.
  TITLE     Bacillus SEVA siblings: A Golden Gate-based toolbox to create 
            personalized integrative vectors for Bacillus subtilis
  JOURNAL   Sci Rep 7 (1), 14134 (2017)
  PUBMED    29074996
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Radeck J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2017) Biology, Technische Universitat Dresden, 
            Zellescher Weg 20b, Dresden 01627, Germany
REFERENCE   3  (bases 1 to 4556)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4556)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2017"; volume: "7"; issue: "1"; pages: "14134"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-APR-2017) Biology, Technische Universitat Dresden, Zellescher 
            Weg 20b, Dresden 01627, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    125..146
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        161..191
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    199..215
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     223..239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    249..305
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(309..325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      604..698
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             877..1689
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            1859..1967
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2002..2590
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2776..2916)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(3021..3209)
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3491..3518)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(3533..4324)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     terminator      complement(4464..4550)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
       61 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      241 atgattacga attcgagctc ggtacccggg gatcctctag agtcgacctg caggcatgca
      301 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      361 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      421 accgatcgcc cttcctaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      481 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      541 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgaactagtc
      601 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
      661 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aggggtcccc aataattacg
      721 atttgagatg agaccttgca ggtgagatga gacggtcttc aacatcgcag taaatttgtg
      781 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
      841 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca gcgtgaaacg
      901 agctgtagcc gtccgcgtct gaacagcaac atggatgcgg atctgtatgg ctataaatgg
      961 gcgcgtgata acgtgggtca gagcggcgcg accatttatc gtctgtatgg caaaccggat
     1021 gcgccggaac tgtttctgaa acatggcaaa ggcagcgtgg cgaacgatgt gaccgatgaa
     1081 atggtgcgtc tgaactggct gaccgaattt atgccgctgc cgaccattaa acattttatt
     1141 cgcaccccgg atgatgcgtg gctgctgacc accgcgattc cgggcaaaac cgcgtttcag
     1201 gtgctggaag aatatccgga tagcggcgaa aacattgtgg atgcgctggc cgtgtttctg
     1261 cgtcgtctgc atagcattcc ggtgtgcaac tgcccgttta acagcgatcg tgtgtttcgt
     1321 ctggcccagg cgcagagccg tatgaacaac ggcctggtgg atgcgagcga ttttgatgat
     1381 gaacgtaacg gctggccggt ggaacaggtg tggaaagaaa tgcataaact gctgccgttt
     1441 agcccggata gcgtggtgac ccacggcgat tttagcctgg ataacctgat tttcgatgaa
     1501 ggcaaactga ttggctgcat tgatgtgggc cgtgtgggca ttgcggatcg ttatcaggat
     1561 ctggccattc tgtggaactg cctgggcgaa tttagcccga gcctgcaaaa acgtctgttt
     1621 cagaaatatg gcattgataa tccggatatg aacaaactgc aatttcatct gatgctggat
     1681 gaatttttct aataattaat tggaccgcgg tccgcgcgtt gtccttttcc gctgcataac
     1741 cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata
     1801 caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg
     1861 gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt
     1921 cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccgtag gccggcccgt
     1981 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2041 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2101 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     2161 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2221 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2281 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2341 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2401 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2461 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2521 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2581 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2641 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     2701 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     2761 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     2821 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     2881 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     2941 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgacgctc
     3001 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg
     3061 cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg
     3121 tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg
     3181 ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc
     3241 atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat
     3301 gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg
     3361 gatattggcg cgttcacctg cgatggcgaa gacttgcgag gtctcagcga tcgtctcagc
     3421 gaggcgcgta aaataataaa aaagccggat taataatctg gctttttata ttctctctct
     3481 agtatataaa cgcagaaagg cccacccgaa ggtgagccag tgtgaggtac taaaacaatt
     3541 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
     3601 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
     3661 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
     3721 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
     3781 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
     3841 cctcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
     3901 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
     3961 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
     4021 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
     4081 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
     4141 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
     4201 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
     4261 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
     4321 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
     4381 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaacg
     4441 cgcccagctg tctagggcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
     4501 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcct
//
