LOCUS       40924_7361        4106 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBSc291K, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Radeck J, Meyer D, Lautenschlager N, Mascher T.
  TITLE     Bacillus SEVA siblings: A Golden Gate-based toolbox to create 
            personalized integrative vectors for Bacillus subtilis
  JOURNAL   Sci Rep 7 (1), 14134 (2017)
  PUBMED    29074996
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Radeck J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2017) Biology, Technische Universitat Dresden, 
            Zellescher Weg 20b, Dresden 01627, Germany
REFERENCE   3  (bases 1 to 4106)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4106)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2017"; volume: "7"; issue: "1"; pages: "14134"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-APR-2017) Biology, Technische Universitat Dresden, Zellescher 
            Weg 20b, Dresden 01627, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      155..249
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             428..1240
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            1410..1518
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      1553..2141
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2327..2467)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2572..2760)
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3083..3874)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     terminator      complement(4014..4100)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttgagat gagaccttgc aggtgagatg
      301 agacggtctt caacatcgca gtaaatttgt gtctcaaaat ctctgatgtt acattgcaca
      361 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
      421 gggtgttatg agccatattc agcgtgaaac gagctgtagc cgtccgcgtc tgaacagcaa
      481 catggatgcg gatctgtatg gctataaatg ggcgcgtgat aacgtgggtc agagcggcgc
      541 gaccatttat cgtctgtatg gcaaaccgga tgcgccggaa ctgtttctga aacatggcaa
      601 aggcagcgtg gcgaacgatg tgaccgatga aatggtgcgt ctgaactggc tgaccgaatt
      661 tatgccgctg ccgaccatta aacattttat tcgcaccccg gatgatgcgt ggctgctgac
      721 caccgcgatt ccgggcaaaa ccgcgtttca ggtgctggaa gaatatccgg atagcggcga
      781 aaacattgtg gatgcgctgg ccgtgtttct gcgtcgtctg catagcattc cggtgtgcaa
      841 ctgcccgttt aacagcgatc gtgtgtttcg tctggcccag gcgcagagcc gtatgaacaa
      901 cggcctggtg gatgcgagcg attttgatga tgaacgtaac ggctggccgg tggaacaggt
      961 gtggaaagaa atgcataaac tgctgccgtt tagcccggat agcgtggtga cccacggcga
     1021 ttttagcctg gataacctga ttttcgatga aggcaaactg attggctgca ttgatgtggg
     1081 ccgtgtgggc attgcggatc gttatcagga tctggccatt ctgtggaact gcctgggcga
     1141 atttagcccg agcctgcaaa aacgtctgtt tcagaaatat ggcattgata atccggatat
     1201 gaacaaactg caatttcatc tgatgctgga tgaatttttc taataattaa ttggaccgcg
     1261 gtccgcgcgt tgtccttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt
     1321 tcggtatatc catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac
     1381 tttccttggt gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg
     1441 agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct
     1501 gctctgcgag gctggccgta ggccggcccg tagaaaagat caaaggatct tcttgagatc
     1561 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     1621 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     1681 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     1741 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     1801 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     1861 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     1921 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     1981 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     2041 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     2161 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     2221 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     2281 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     2341 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     2401 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     2461 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     2521 ggcatccgct tacagacaag ctgtgacgct ctccgggagc tgcatgtgtc agaggttttc
     2581 accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag
     2641 cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt
     2701 taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac
     2761 tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga
     2821 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga
     2881 gggtaaacaa ctggcggtat ggatgcggcg ggatattggc gcgttcacct gcgatggcga
     2941 agacttgcga ggtctcagcg atcgtctcag cgaggcgcgt aaaataataa aaaagccgga
     3001 ttaataatct ggctttttat attctctctc tagtatataa acgcagaaag gcccacccga
     3061 aggtgaccag tgtgaggtac taaaacaatt catccagtaa aatataatat tttattttct
     3121 cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga
     3181 tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc
     3241 ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc
     3301 ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat
     3361 acagctcgcg cggatcttta aatggagtgt cctcttccca gttttcgcaa tccacatcgg
     3421 ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat
     3481 agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga
     3541 taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct
     3601 cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag
     3661 gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc
     3721 ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat
     3781 ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt
     3841 ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca
     3901 gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct
     3961 tgcattctaa aaccttaaat accagaaacg cgcccagctg tctagggcgg cggatttgtc
     4021 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     4081 tgagcctttc gttttatttg atgcct
//
