LOCUS       40924_7311        5291 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBSc241T, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Radeck J, Meyer D, Lautenschlager N, Mascher T.
  TITLE     Bacillus SEVA siblings: A Golden Gate-based toolbox to create 
            personalized integrative vectors for Bacillus subtilis
  JOURNAL   Sci Rep 7 (1), 14134 (2017)
  PUBMED    29074996
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Radeck J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2017) Biology, Technische Universitat Dresden, 
            Zellescher Weg 20b, Dresden 01627, Germany
REFERENCE   3  (bases 1 to 5291)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5291)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2017"; volume: "7"; issue: "1"; pages: "14134"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-APR-2017) Biology, Technische Universitat Dresden, Zellescher 
            Weg 20b, Dresden 01627, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      155..249
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             428..1240
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            1410..1518
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      1612..2200
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2257..3087)
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      3101..3452
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             complement(3615..4988)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="VNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS
                     LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA
                     HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS
                     ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY
                     MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA
                     SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT
                     GIAIVGGLLSIPLLDQRLLTMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF
                     "
     terminator      complement(5199..5285)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttgagat gagaccttgc aggtgagatg
      301 agacggtctt caacatcgca gtaaatttgt gtctcaaaat ctctgatgtt acattgcaca
      361 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
      421 gggtgttatg agccatattc agcgtgaaac gagctgtagc cgtccgcgtc tgaacagcaa
      481 catggatgcg gatctgtatg gctataaatg ggcgcgtgat aacgtgggtc agagcggcgc
      541 gaccatttat cgtctgtatg gcaaaccgga tgcgccggaa ctgtttctga aacatggcaa
      601 aggcagcgtg gcgaacgatg tgaccgatga aatggtgcgt ctgaactggc tgaccgaatt
      661 tatgccgctg ccgaccatta aacattttat tcgcaccccg gatgatgcgt ggctgctgac
      721 caccgcgatt ccgggcaaaa ccgcgtttca ggtgctggaa gaatatccgg atagcggcga
      781 aaacattgtg gatgcgctgg ccgtgtttct gcgtcgtctg catagcattc cggtgtgcaa
      841 ctgcccgttt aacagcgatc gtgtgtttcg tctggcccag gcgcagagcc gtatgaacaa
      901 cggcctggtg gatgcgagcg attttgatga tgaacgtaac ggctggccgg tggaacaggt
      961 gtggaaagaa atgcataaac tgctgccgtt tagcccggat agcgtggtga cccacggcga
     1021 ttttagcctg gataacctga ttttcgatga aggcaaactg attggctgca ttgatgtggg
     1081 ccgtgtgggc attgcggatc gttatcagga tctggccatt ctgtggaact gcctgggcga
     1141 atttagcccg agcctgcaaa aacgtctgtt tcagaaatat ggcattgata atccggatat
     1201 gaacaaactg caatttcatc tgatgctgga tgaatttttc taataattaa ttggaccgcg
     1261 gtccgcgcgt tgtccttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt
     1321 tcggtatatc catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac
     1381 tttccttggt gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg
     1441 agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct
     1501 gctctgcgag gctggccgta ggccggccga taatctcatg accaaaatcc cttaacgtga
     1561 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1621 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1681 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1741 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1801 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1861 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1921 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1981 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2041 ggacaggcat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2101 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2161 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggccgt
     2221 gaaaggcagg ccggtccgtg gtggccacgg cctctaggcc agatccagcg gcatctgggt
     2281 tagtcgagcg cgggccgctt cccatgtctc accagggcga gcctgtttcg cgatctcagc
     2341 atctgaaatc ttcccggcct tgcgcttcgc tggggcctta cccaccgcct tggcgggctt
     2401 cttcggtcca aaactgaaca acagatgtgt gaccttgcgc ccggtctttc gctgcgccca
     2461 ctccacctgt agcgggctgt gctcgttgat ctgcgtcacg gctggatcaa gcactcgcaa
     2521 cttgaagtcc ttgatcgagg gataccggcc ttccagttga aaccactttc gcagctggtc
     2581 aatttctatt tcgcgctggc cgatgctgtc ccattgcatg agcagctcgt aaagcctgat
     2641 cgcgtgggtg ctgtccatct tggccacgtc agccaaggcg tatttggtga actgtttggt
     2701 gagttccgtc aggtacggca gcatgtcttt ggtgaacctg agttctacac ggccctcacc
     2761 ctcccggtag atgattgttt gcacccagcc ggtaatcatc acactcggtc ttttcccctt
     2821 gccattgggc tcttgggtta accggacttc ccgccgtttc aggcgcaggg ccgcttcttt
     2881 gagctggttg taggaagatt cgatagggac acccgccatc gtcgctatgt cctccgccgt
     2941 cactgaatac atcacttcat cggtgacagg ctcgctcctc ttcacctggc taatacaggc
     3001 cagaacgatc cgctgttcct gaacactgag gcgatacgcg gcctcgacca gggcattgct
     3061 tttgtaaacc attgggggtg aggccacgtt cgacattcct tgtgtataag gggacactgt
     3121 atctgcgtcc cacaatacaa caaatccgtc cctttacaac aacaaatccg tcccttctta
     3181 acaacaaatc cgtcccttaa tggcaacaaa tccgtccctt tttaaactct acaggccacg
     3241 gattacgtgg cctgtagacg tcctaaaagg tttaaaaggg aaaaggaaga aaagggtgga
     3301 aacgcaaaaa acgcaccact acgtggcccc gttggggccg catttgtgcc cctgaagggg
     3361 cgggggaggc gtctgggcaa tccccgtttt accagtcccc tatcgccgcc tgagagggcg
     3421 caggaagcga gtaatcaggg tatcgaggcg gattcaccct tggcgtccaa ccagcggcac
     3481 cagcggcgcc tgagaggggc gcgttcacct gcgatggcga agacttgcga ggtctcagcg
     3541 atcgtctcag cgaggcgcgt taaaaaaagg atcaattttg aactctctcc caaagttgat
     3601 cccttaacga tttagaaatc cctttgagaa tgtttatata cattcaaggt aaccagccaa
     3661 ctaatgacaa tgattcctga aaaaagtaat aacaaattac tatacagata agttgactga
     3721 tcaacttcca tagttaacaa cctttgatca agtaagggta tggataataa accacctaca
     3781 attgcaatac ctgttccctc tgataaaaag ctggtaaagt taagcaaact cattccagca
     3841 ccagcttcct gctgtttcaa gctacttgaa acaattgttg atataactgt tttggtgaac
     3901 gaaagcccac ctaaaacaaa tacgattata attgtcatga accatgatgt tgtttctaaa
     3961 agaaaggaag cagttaaaaa gctaacagaa agaaatgtaa ctccgatgtt taacacgtat
     4021 aaaggacctc ttctatcaac aagtatccca ccaatgtagc cgaaaataat gacactcatt
     4081 gttccaggga aaataattac acttccgatt tcggcagtac ttagctggtg aacatctttc
     4141 atcatataag gaaccataga gacaaaccct gctactgttc caaatataat tcccccacaa
     4201 agaactccaa tcataaaagg tatatttttc cctaatccgg gatcaacaaa aggatctgtt
     4261 actttcctga tatgttttac aaatatcagg aatgacagca cgctaacgat aagaaaagaa
     4321 atgctatatg atgttgtaaa caacataaaa aatacaatgc ctacagacat tagtataatt
     4381 cctttgatat caaaatgacc ttttatcctt acttctttct ttaataattt cataagaaac
     4441 ggaacagtga taattgttat cataggaatg agtagaagat aggaccaatg aatataatgg
     4501 gctatcattc caccaatcgc tggaccgact ccttctccca tggctactat cgatccaata
     4561 agaccaaatg ctttacccct attttccttt ggaatatagc gcgcaactac aaccattacg
     4621 agtgctggaa atgcagctgc accagcccct tgaataaaac gagccataat aagtaaggaa
     4681 aagaaagaat ggccaacaaa cccaattacc gacccgaaac aatttattat aattccaaat
     4741 aggagtaacc ttttgatgcc taattgatca gatagctttc catatacagc tgttccaatg
     4801 gaaaaggtta acataaaggc tgtgttcacc cagtttgtac tcgcaggagg tttattaaaa
     4861 tcatttgcaa tatcaggtaa gctgacgttc aaaaccattt catttaatac gctaaaaaaa
     4921 gataaaatgc aaagccaaat taaaatttgg ttgtgtcgta aattcgattg tgaataggat
     4981 gtattcacat ttcaccctcc aataatgagg gcagacgtag tttatagggt taatgatacg
     5041 cttccctctt ttaattgaac cctgttacat taattacact tcataattaa ttcctcctaa
     5101 acttgattaa aacattttac cacatataaa ctaagtttca gattcttttc ttcatcatcg
     5161 gtcataaaat ccacgcgccc agctgtctag ggcggcggat ttgtcctact caggagagcg
     5221 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt
     5281 atttgatgcc t
//
