LOCUS       V009057                 4520 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009057
VERSION     V009057
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Radeck J, Meyer D, Lautenschlager N, Mascher T.
  TITLE     Bacillus SEVA siblings: A Golden Gate-based toolbox to create
            personalized integrative vectors for Bacillus subtilis
  JOURNAL   Sci Rep 7 (1), 14134 (2017)
   PUBMED   29074996
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Radeck J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2017) Biology, Technische Universitat Dresden,
            Zellescher Weg 20b, Dresden 01627, Germany
REFERENCE   3  (bases 1 to 4520)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4520)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep";
            date: "2017"; volume: "7"; issue: "1"; pages: "14134"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-APR-2017) Biology, Technische Universitat Dresden, Zellescher
            Weg 20b, Dresden 01627, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    56..112
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     terminator      155..249
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             428..1240
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     oriT            1410..1518
                     /label="oriT"
                     /note="incP origin of transfer"
     rep_origin      1612..2200
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2257..3087)
                     /label="pRO1600 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     rep_origin      3101..3452
                     /label="pRO1600 oriV"
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             complement(3620..4267)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     terminator      complement(4428..4514)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttgagat gagaccttgc aggtgagatg
      301 agacggtctt caacatcgca gtaaatttgt gtctcaaaat ctctgatgtt acattgcaca
      361 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
      421 gggtgttatg agccatattc agcgtgaaac gagctgtagc cgtccgcgtc tgaacagcaa
      481 catggatgcg gatctgtatg gctataaatg ggcgcgtgat aacgtgggtc agagcggcgc
      541 gaccatttat cgtctgtatg gcaaaccgga tgcgccggaa ctgtttctga aacatggcaa
      601 aggcagcgtg gcgaacgatg tgaccgatga aatggtgcgt ctgaactggc tgaccgaatt
      661 tatgccgctg ccgaccatta aacattttat tcgcaccccg gatgatgcgt ggctgctgac
      721 caccgcgatt ccgggcaaaa ccgcgtttca ggtgctggaa gaatatccgg atagcggcga
      781 aaacattgtg gatgcgctgg ccgtgtttct gcgtcgtctg catagcattc cggtgtgcaa
      841 ctgcccgttt aacagcgatc gtgtgtttcg tctggcccag gcgcagagcc gtatgaacaa
      901 cggcctggtg gatgcgagcg attttgatga tgaacgtaac ggctggccgg tggaacaggt
      961 gtggaaagaa atgcataaac tgctgccgtt tagcccggat agcgtggtga cccacggcga
     1021 ttttagcctg gataacctga ttttcgatga aggcaaactg attggctgca ttgatgtggg
     1081 ccgtgtgggc attgcggatc gttatcagga tctggccatt ctgtggaact gcctgggcga
     1141 atttagcccg agcctgcaaa aacgtctgtt tcagaaatat ggcattgata atccggatat
     1201 gaacaaactg caatttcatc tgatgctgga tgaatttttc taataattaa ttggaccgcg
     1261 gtccgcgcgt tgtccttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt
     1321 tcggtatatc catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac
     1381 tttccttggt gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg
     1441 agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct
     1501 gctctgcgag gctggccgta ggccggccga taatctcatg accaaaatcc cttaacgtga
     1561 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1621 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1681 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1741 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1801 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1861 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1921 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1981 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2041 ggacaggcat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2101 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2161 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggccgt
     2221 gaaaggcagg ccggtccgtg gtggccacgg cctctaggcc agatccagcg gcatctgggt
     2281 tagtcgagcg cgggccgctt cccatgtctc accagggcga gcctgtttcg cgatctcagc
     2341 atctgaaatc ttcccggcct tgcgcttcgc tggggcctta cccaccgcct tggcgggctt
     2401 cttcggtcca aaactgaaca acagatgtgt gaccttgcgc ccggtctttc gctgcgccca
     2461 ctccacctgt agcgggctgt gctcgttgat ctgcgtcacg gctggatcaa gcactcgcaa
     2521 cttgaagtcc ttgatcgagg gataccggcc ttccagttga aaccactttc gcagctggtc
     2581 aatttctatt tcgcgctggc cgatgctgtc ccattgcatg agcagctcgt aaagcctgat
     2641 cgcgtgggtg ctgtccatct tggccacgtc agccaaggcg tatttggtga actgtttggt
     2701 gagttccgtc aggtacggca gcatgtcttt ggtgaacctg agttctacac ggccctcacc
     2761 ctcccggtag atgattgttt gcacccagcc ggtaatcatc acactcggtc ttttcccctt
     2821 gccattgggc tcttgggtta accggacttc ccgccgtttc aggcgcaggg ccgcttcttt
     2881 gagctggttg taggaagatt cgatagggac acccgccatc gtcgctatgt cctccgccgt
     2941 cactgaatac atcacttcat cggtgacagg ctcgctcctc ttcacctggc taatacaggc
     3001 cagaacgatc cgctgttcct gaacactgag gcgatacgcg gcctcgacca gggcattgct
     3061 tttgtaaacc attgggggtg aggccacgtt cgacattcct tgtgtataag gggacactgt
     3121 atctgcgtcc cacaatacaa caaatccgtc cctttacaac aacaaatccg tcccttctta
     3181 acaacaaatc cgtcccttaa tggcaacaaa tccgtccctt tttaaactct acaggccacg
     3241 gattacgtgg cctgtagacg tcctaaaagg tttaaaaggg aaaaggaaga aaagggtgga
     3301 aacgcaaaaa acgcaccact acgtggcccc gttggggccg catttgtgcc cctgaagggg
     3361 cgggggaggc gtctgggcaa tccccgtttt accagtcccc tatcgccgcc tgagagggcg
     3421 caggaagcga gtaatcaggg tatcgaggcg gattcaccct tggcgtccaa ccagcggcac
     3481 cagcggcgcc tgagaggggc gcgttcacct gcgatggcga agacttgcga ggtctcagcg
     3541 atcgtctcag cgaggcgcgt caggttagtg acattagaaa accgactgta aaaagtacag
     3601 tcggcattat ctcatattat aaaagccagt cattaggcct atctgacaat tcctgaatag
     3661 agttcataaa caatcctgca tgataaccat cacaaacaga atgatgtacc tgtaaagata
     3721 gcggtaaata tattgaatta cctttattaa tgaattttcc tgctgtaata atgggtagaa
     3781 ggtaattact attattattg atatttaagt taaacccagt aaatgaagtc catggaataa
     3841 tagaaagaga aaaagcattt tcaggtatag gtgttttggg aaacaatttc cccgaaccat
     3901 tatatttctc tacatcagaa aggtataaat cataaaactc tttgaagtca ttctttacag
     3961 gagtccaaat accagagaat gttttagata caccatcaaa aattgtataa agtggctcta
     4021 acttatccca ataacctaac tctccgtcgc tattgtaacc agttctaaaa gctgtatttg
     4081 agtttatcac ccttgtcact aagaaaataa atgcagggta aaatttatat ccttcttgtt
     4141 ttatgtttcg gtataaaaca ctaatatcaa tttctgtggt tatactaaaa gtcgtttgtt
     4201 ggttcaaata atgattaaat atctcttttc tcttccaatt gtctaaatca attttattaa
     4261 agttcatttg atatgcctcc taaattttta tctaaagtga atttaggagg cttacttgtc
     4321 tgctttcttc attagaatca atcctttttt aaaagtcaat attactgtaa cataaatata
     4381 tattttaaaa atatcccact tacgcgccca gctgtctagg gcggcggatt tgtcctactc
     4441 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc
     4501 tttcgtttta tttgatgcct
//
