LOCUS       40924_7466        3795 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBSTG1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Tiede C, Tang AA, Deacon SE, Mandal U, Nettleship JE, Owen RL, 
            George SE, Harrison DJ, Owens RJ, Tomlinson DC, McPherson MJ.
  TITLE     Adhiron: a stable and versatile peptide display scaffold for 
            molecular recognition applications
  JOURNAL   Protein Eng. Des. Sel. 27 (5), 145-155 (2014)
  PUBMED    24668773
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Deacon SE, Tiede C, Tomlinson DC, McPherson MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2014) School of Molecular and Cellular Biology, 
            University of Leeds, Astbury Building, Leeds, West Yorkshire WF12 
            0LN, UK
REFERENCE   3  (bases 1 to 3795)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3795)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Eng. Des. Sel."; date: "2014"; volume: "27"; issue: "5"; pages: 
            "145-155"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-FEB-2014) School of Molecular and Cellular Biology, University 
            of Leeds, Astbury Building, Leeds, West Yorkshire WF12 0LN, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..31
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    39..55
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     63..79
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    131..187
                     /label=DsbA secretion signal sequence
                     /note="DsbA secretion signal sequence"
     CDS             236..253
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    257..301
                     /note="flexible linker between oligohistidine tag and
                     truncated M13 gp3 coding region"
     CDS             302..772
                     /codon_start=1
                     /product="truncated gp3"
                     /label=truncated gp3
                     /note="minor coat protein"
                     /protein_id="AIE57570.1"
                     /translation="GGSGSGDFDYEKMANANKGAMTENADENALQSDAKGKLDSVATDY
                     GAAIDGFIGDVSGLANGNGATGDFAGSNSQMAQVGDGDNSPLMNNFRQYLPSLPQSVEC
                     RPYVFGAGKPYEFSIDCDKINLFRGVFAFLLYVATFMYVFSTFANILRNKES"
     primer_bind     complement(782..798)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1011..1466
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1748..1852
                     /label=AmpR promoter
     CDS             1853..2710
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2884..3472
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3760..3781
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
       61 cacaggaaac agctatgacc atgattacgc caagcttgca tgcaaattct atttcaagga
      121 gacagtcata atgaaaaaga tttggttggc tctggctggt ctggttctgg cgttttctgc
      181 gtctgctagc caggtgcagc tgcaggtcga cctcgagatc aaacgggcgg ccgcgcatca
      241 ccatcatcac cactagggta acggtggtgg taacggtaac tctggtggtg gttctggtac
      301 cggcggctcc ggttccggtg attttgatta tgaaaaaatg gcaaacgcta ataagggggc
      361 tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca aacttgattc
      421 tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt ccggccttgc
      481 taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg ctcaagtcgg
      541 tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt ctttgcctca
      601 gtcggttgaa tgtcgccctt atgtctttgg cgctggtaaa ccatatgaat tttctattga
      661 ttgtgacaaa ataaacttat tccgtggtgt ctttgcgttt cttttatatg ttgccacctt
      721 tatgtatgta ttttcgacgt ttgctaacat actgcgtaat aaggagtctt aataagaatt
      781 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      841 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      901 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
      961 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     1021 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     1081 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     1141 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     1201 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     1261 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     1321 ccaaactgga acaacactca accctatctc gggctattct tttgatttat aagggatttt
     1381 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     1441 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     1501 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     1561 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     1621 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     1681 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     1741 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1801 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     1861 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     1921 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     1981 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2041 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     2101 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2161 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2221 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     2281 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2341 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2401 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     2461 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2521 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2581 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2641 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2701 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2761 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2821 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2881 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2941 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3001 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     3061 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3121 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3181 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3241 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3301 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3361 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3421 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3481 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3541 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3601 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     3661 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     3721 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     3781 ttaggcaccc caggc
//
