LOCUS       40924_7461        4240 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBSPPc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Xu Y, Tao F, Ma C, Xu P.
  TITLE     New constitutive vectors: useful genetic engineering tools for 
            biocatalysis
  JOURNAL   Appl. Environ. Microbiol. 79 (8), 2836-2840 (2013)
  PUBMED    23416993
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Xu Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2013) State Key Laboratory of Microbial 
            Technology, Shandong University, South Shanda Road, Jinan, Shandong 
            250100, China
REFERENCE   3  (bases 1 to 4240)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4240)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2013"; volume: "79"; issue: "8"; pages:
            "2836-2840"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-JAN-2013) State Key Laboratory of Microbial Technology, Shandong
            University, South Shanda Road, Jinan, Shandong 250100, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(415..766)
                     /direction=LEFT
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             780..1610
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     primer_bind     1774..1790
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1800..1818
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2060..2076
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2110..2126)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2163..2181)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2202..2218)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2226..2242)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2250..2280)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2295..2316)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2604..3192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3366..4223)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(join(4224..4240,1..88))
                     /label=AmpR promoter
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
      121 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
      181 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
      241 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
      301 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
      361 tgcaccatac ctctcaggcg ccgctggtgc cgctggttgg acgccaaggg tgaatccgcc
      421 tcgataccct gattactcgc ttcctgcgcc ctctcaggcg gcgatagggg actggtaaaa
      481 cggggattgc ccagacgcct cccccgcccc ttcaggggca caaatgcggc cccaacgggg
      541 ccacgtagtg gtgcgttttt tgcgtttcca cccttttctt ccttttccct tttaaacctt
      601 ttaggacgtc tacaggccac gtaatccgtg gcctgtagag tttaaaaagg gacggatttg
      661 ttgccattaa gggacggatt tgttgttaag aagggacgga tttgttgttg taaagggacg
      721 gatttgttgt attgtgggac gcagatacag tgtcccctta tacacaagga atgtcgaaca
      781 tggcctcacc cccaatggtt tacaaaagca atgccctggt cgaggccgcg tatcgcctca
      841 gtgttcagga acagcggatc gttctggcct gtattagcca ggtgaagagg agcgagcctg
      901 tcaccgatga agtgatgtat tcagtgacgg cggaggacat agcgacgatg gcgggtgtcc
      961 ctatcgaatc ttcctacaac cagctcaaag aagcggccct gcgcctgaaa cggcgggaag
     1021 tccggttaac ccaagagccc aatggcaagg ggaaaagacc gagtgtgatg attaccggct
     1081 gggtgcaaac aatcatctac cgggagggtg agggccgtgt agaactcagg ttcaccaaag
     1141 acatgctgcc gtacctgacg gaactcacca aacagttcac caaatacgcc ttggctgacg
     1201 tggccaagat ggacagcacc cacgcgatca ggctttacga gctgctcatg caatgggaca
     1261 gcatcggcca gcgcgaaata gaaattgacc agctgcgaaa gtggtttcaa ctggaaggcc
     1321 ggtatccctc gatcaaggac ttcaagttgc gagtgcttga tccagccgtg acgcagatca
     1381 acgagcacag cccgctacag gtggagtggg cgcagcgaaa gaccgggcgc aaggtcacac
     1441 atctgttgtt cagttttgga ccgaagaagc ccgccaaggc ggtgggtaag gccccagcga
     1501 agcgcaaggc cgggaagatt tcagatgctg agatcgcgaa acaggctcgc cctggtgaga
     1561 catgggaagc ggcccgcgct cgactaaccc agatgccgct ggatctggcc tagaggccgt
     1621 ggccaccacg gcccggcctg cctttcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     1681 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     1741 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt
     1801 aatacgactc actatagggc gaattgggta cctgccgata caagaacaac agcgcgttga
     1861 gcgcctgccg gtgggtggcc ggcgccactt gcttctcggt ggcgagcatg gtcagaaaac
     1921 cctcgacttc agcttgcccc atttcgcgcg gatgtcgaaa cccaccatgg ctgcgggccg
     1981 tccacaacac aaatgccttg gcccagtaga cataagcctt ctcggtctgt aggctgtaat
     2041 gcaggtagcg aacccgtctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc
     2101 agcccggggg atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt
     2161 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     2221 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     2281 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     2341 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     2401 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2461 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2521 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2581 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2641 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2701 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2761 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2821 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2881 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2941 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3001 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     3061 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3121 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3181 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3241 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3301 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3361 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3421 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3481 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3541 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3601 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3661 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3721 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3781 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3841 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3901 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3961 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     4021 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4081 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4141 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4201 acacggaaat gttgaatact catactcttc ctttttcaat
//
