LOCUS       62056_4195        3093 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        36..54
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(313..331)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(338..354)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      496..951
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        982..1086
                     /label=AmpR promoter
     CDS             1087..1944
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2118..2706
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2994..3015
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3030..3060
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3068..3084
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggaaacagct atgaccatga ttacgccaag ctcgaaatta accctcacta aagggaacaa
       61 aagctggtac cgggcccccc ctcgaggtcg ccccatcaaa aaaatattct caacataaaa
      121 aactttgtgt aatacttgta acgctgaatt cgcccggata gctcagtcgg tagagcagcg
      181 gccgatatcc gcgggtccag ggttcaagtc cctgttcggg cgccactgca gatccttagc
      241 gaaagctaag gatttttttt aagcttgatg ggggcttagg atccggagct ccaagcttta
      301 gctccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
      361 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
      421 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
      481 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      541 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      601 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
      661 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
      721 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
      781 cgttctttaa tagtggactc ttgttccaaa cttgaacaac actcaaccct atctcgggct
      841 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
      901 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt taaatcaggt
      961 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     1021 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     1081 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     1141 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     1201 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     1261 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     1321 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     1381 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     1441 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     1501 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     1561 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     1621 acgatgccag cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     1681 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     1741 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     1801 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     1861 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     1921 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     1981 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     2041 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     2101 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2161 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2221 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     2281 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2341 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2401 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2461 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     2521 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2581 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2641 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2701 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2761 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2821 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2881 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     2941 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     3001 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     3061 tgtggaattg tgagcggata acaatttcac aca
//
