LOCUS       40924_7451        4445 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBSK-mBC4-Sense-GUS-Antisense, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Narwade AV, Chakrabarty PK, Kalbande BB, Chavhan RL, Warade JG, 
            Sable SV, Nandeshwar SB.
  TITLE     Inverted repeat construct for RNA interference-mediated targeting of
            beta-C4 protein gene of Cotton Leaf Curl Virus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Narwade AV, Chakrabarty PK, Kalbande BB, Chavhan RL, Warade JG, 
            Sable SV, Nandeshwar SB.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2010) Division of Crop Improvement, Central 
            Institute for Cotton Research, Post Bag No. 02, Shankar Nagar P.O., 
            Nagpur, Maharashtra 440010, India
REFERENCE   3  (bases 1 to 4445)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4445)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-DEC-2010) Division of Crop Improvement, Central Institute for 
            Cotton Research, Post Bag No. 02, Shankar Nagar P.O., Nagpur, 
            Maharashtra 440010, India"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    653..2244
                     /note="MCS; multiple cloning site"
     misc_feature    659..991
                     /note="mBC4-sense strand; modified beta-C4 protein fragment
                     of Cotton Leaf Curl Virus isolate Sirsa (Haryana) segment 
                     beta-DNA deposite in GenBank Accession NumberHM146308; 
                     cloned in sense orientation"
     misc_feature    998..1875
                     /note="beta-glucuronidase; Escherichia coli GusA (N358Q) 
                     fragment from pCAMBIA-1302 was PCR amplified with 
                     engineered restriction sites and cloned in EcoRI and BamHI 
                     sites of the MCS of lacZ' in pBluscriptII SK(+) (X52328.1)"
     misc_feature    1882..2214
                     /note="mBC4-antisense strand; modified beta-C4 protein
                     fragment of Cotton Leaf Curl Virus isolate Sirsa (Haryana) 
                     segment beta-DNA deposite in GenBank Accession 
                     NumberHM146308; cloned in antisense orientation"
     promoter        complement(2257..2275)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2296..2312)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2320..2336)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2344..2374)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2389..2410)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2698..3286)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3460..4317)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4318..4422)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgc
      661 agaaatgaca acgagcggaa caaacaagaa gggagtcagg ttcatagtcg acgttcgcat
      721 catggagaat atgaagatct tcattcacat gaggatacta tccacaaagt caccatcgct
      781 aatcaagtat gaagggatcg tccagtacac gtacgaagac atacacgttc cattcgactt
      841 caacggcttt gaagggagca tcatagcgaa tttcctattc gcctacaacg aggcaaagat
      901 agaagagatc gagatagaag atatagttca caggcttgat atacttgtac ttgaaaaccc
      961 aagatattgg gaatggatgt aacaaccgta cgaattccgc cgggaatggt gattaccgac
     1021 gaaaacggca agaaaaagca gtcttacttc catgatttct ttaactatgc cggaatccat
     1081 cgcagcgtaa tgctctacac cacgccgaac acctgggtgg acgatatcac cgtggtgacg
     1141 catgtcgcgc aagactgtaa ccacgcgtct gttgactggc aggtggtggc caatggtgat
     1201 gtcagcgttg aactgcgtga tgcggatcaa caggtggttg caactggaca aggcactagc
     1261 gggactttgc aagtggtgaa tccgcacctc tggcaaccgg gtgaaggtta tctctatgaa
     1321 ctgtgcgtca cagccaaaag ccagacagag tgtgatatct acccgcttcg cgtcggcatc
     1381 cggtcagtgg cagtgaaggg cgaacagttc ctgattaacc acaaaccgtt ctactttact
     1441 ggctttggtc gtcatgaaga tgcggacttg cgtggcaaag gattcgataa cgtgctgatg
     1501 gtgcacgacc acgcattaat ggactggatt ggggccaact cctaccgtac ctcgcattac
     1561 ccttacgctg aagagatgct cgactgggca gatgaacatg gcatcgtggt gattgacgaa
     1621 actgctgctg tcggctttaa cctctcttta ggcattggtt tcgaagcggg caacaagccg
     1681 aaagaactgt acagcgaaga ggcagtcaac ggggaaactc agcaagcgca cttacaggcg
     1741 attaaagagc tgatagcgcg tgacaaaaac cacccaagcg tggtgatgtg gagtattgcc
     1801 aacgaaccgg atacccgtcc gcaaggtgca cgggaatatt tcgcgccact ggcggaagca
     1861 acgcgtaaac tcgacggatc cgtacggttg ttacatccat tcccaatatc ttgggttttc
     1921 aagtacaagt atatcaagcc tgtgaactat atcttctatc tcgatctctt ctatctttgc
     1981 ctcgttgtag gcgaatagga aattcgctat gatgctccct tcaaagccgt tgaagtcgaa
     2041 tggaacgtgt atgtcttcgt acgtgtactg gacgatccct tcatacttga ttagcgatgg
     2101 tgactttgtg gatagtatcc tcatgtgaat gaagatcttc atattctcca tgatgcgaac
     2161 gtcgactatg aacctgactc ccttcttgtt tgttccgctc gttgtcattt ctgctctaga
     2221 gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg
     2281 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     2341 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     2401 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     2461 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     2521 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2581 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2641 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2701 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2761 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2821 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2881 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2941 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3001 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3061 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3121 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3181 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3241 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3301 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3361 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3421 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3481 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3541 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3601 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3661 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3721 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3781 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3841 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3901 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3961 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4021 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4081 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4141 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4201 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4261 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     4321 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     4381 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     4441 gccac
//
