LOCUS       40924_7416        7769 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pBSJL2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Liu J, Xie Z, Merritt J, Qi F.
  TITLE     Establishment of a Tractable Genetic Transformation System in 
            Veillonella spp
  JOURNAL   Appl. Environ. Microbiol. 78 (9), 3488-3491 (2012)
  PUBMED    22344660
REFERENCE   2  (bases 1 to 7769)
  AUTHORS   Liu J.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 7769)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7769)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "9"; pages:
            "3488-3491"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-JAN-2012) Microbiology "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1000..2679)
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /label=rep
                     /protein_id="AFC98318.1"
                     /translation="MSDIVMTDTELYDAKVNWFKEHDYVEVEPLAFYRDLYPIGSFQVK
                     GDYSRKCGNGILLYRNEGKFKHRYIFDDLEEIKNWIGKEDIFVRGGSFIGKRVKNENAS
                     MIYALTFDLDGQGMNELEMVMQFMRNGTNIPMATYVVLSGHGLHLYYLLDKPIRATQDT
                     IRQLNKLKEGMTKLIWNRYTSTIEKPQYQYCLQGFRMVGSASKMGANYPVRAYKTGDRM
                     TIEKLVSWIPRLKDWDEYRINLTERSTVSLAKAKEIWPDWYERKIVKQEDNKWHINRAL
                     YDWWLNKIKEGATYGHRYFCIMCLAIYAIKCNISEAELKRDAYSLLDKMNELSKKGDPS
                     SQFTKMDIEAALQGYKEHFKTWGRDKIALISAIPLPINKRNYRTQEEHMKVISAIRDIV
                     KPNWREGNGRPKGCKNSNYPKAEIVKQWCLDHPNGKKIDCHRDTGLSRMTINKWWNIST
                     EELKRQKIINKIEFIRNKTIQLNETPEVLYPNTSEEIAKMRKNPDTVLELNRLGISFEQ
                     LENQHEIYVEDSVTFQELIEKEFGDDWIDALNLYLDYKMERK"
     gene            complement(1000..2679)
                     /gene="rep"
                     /label=rep
     misc_feature    complement(2680..3234)
                     /label=derived from Veillonella plasmid pVJL1
                     /note="derived from Veillonella plasmid pVJL1"
     CDS             complement(3376..5295)
                     /codon_start=1
                     /gene="tet(M)"
                     /product="tetracycline resistance protein Tet(M)"
                     /label=tet(M)
                     /protein_id="AFC98319.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDRGTTKTDN
                     TLLERQRGITIQTAITSFQWKNTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDG
                     VQAQTRILFHALRKIGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELHPN
                     MRVMNFTESEQWDMVIEGNDYLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYHGSAK
                     NNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSV
                     RISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIE
                     NPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLGKVQMEVT
                     CALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVAQLPLGSGV
                     QYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFR
                     MLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVI
                     LSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMF
                     NKIT"
     gene            complement(3376..5295)
                     /gene="tet(M)"
                     /label=tet(M)
     misc_feature    complement(5296..5508)
                     /note="promoter region of gyrA from Veillonella parvula
                     PK1910"
     primer_bind     complement(5535..5551)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(5581..5599)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5620..5636)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5644..5660)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5668..5698)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5713..5734)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6022..6610)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6784..7641)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7642..7746)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcgggtag agatgccaat attccattaa ccttatcaaa taaagaaaat gattacatta
      721 taatcataga gaataaatat cttagattat taaaatactg cattatactc caagtaatag
      781 ctaatgaaga aaaagaaaaa attagctcta ctgataacct ctaatcaaaa taaaaaagga
      841 tagtcctttc attaattaag gactatcctt tttttatttt taaaaatata aggcttactt
      901 taataattta tacagtattc ttgattttcc catcgttcca tataccatct ccataaaatc
      961 cataaccacc atctataaat tgtcgcacat attctgcact tatttacgct ccattttgta
     1021 gtccaaataa agatttaaag catcaatcca atcatcacca aattcttttt ctattagttc
     1081 ctgaaacgta acactatctt ctacataaat ttcatgttga ttttctagtt gctcaaagct
     1141 tatccctaat cgatttagct ctaaaactgt atctggattc ttcctcattt ttgcaatctc
     1201 ttctgaagta ttaggatata gaacttcagg agtttcattt aattgtatgg tcttatttct
     1261 gataaactca atcttattta ttattttttg tcgttttaat tcttccgtcg atatattcca
     1321 ccatttatta atcgtcattc gtgataatcc agtatctcga tgacaatcaa ttttcttacc
     1381 attaggatga tctaaacacc attgctttac aatctcagct ttaggataat tggagttttt
     1441 acatcctttt ggtcggccat tgccttcacg ccaattaggt ttaactatat ccctaatagc
     1501 actaataact ttcatatgct cttcttgtgt acgataattt cgcttattaa tgggtaaagg
     1561 aatagctgat attaatgcaa ttttatccct tccccaggtt ttaaaatgtt ctttataccc
     1621 ttgaagtgct gcctctatat ccatcttcgt aaattgacta gatggatctc cttttttaga
     1681 taactcattc atcttatcta gtaaagaata agcatctcgt tttaattctg cctctgatat
     1741 gttgcattta atcgcataaa ttgctaaaca cataatacaa aaatacctat gcccataagt
     1801 tgcgccttct tttattttat ttaaccacca atcatataaa gcacgattaa tatgccattt
     1861 attatcttct tgcttcacta tttttctttc ataccaatct ggccagattt ctttcgcttt
     1921 tgctaaagaa acagtagaac gttcagttaa attaattcga tattcgtccc aatcttttaa
     1981 tctaggtatc caagatacta atttttcaat cgtcatacga tcaccagtct tataagctcg
     2041 tacaggataa ttagcaccca ttttactcgc agatccaacc attctaaaac cttgcaaaca
     2101 gtactgatat tgtggttttt caatagttga tgtatatcga ttccaaatca atttagtcat
     2161 accttctttt aacttattta actgacgaat cgtatcttgc gtcgctcgaa taggcttatc
     2221 caataaataa tataaatgaa gtccatgacc acttaataca acatatgttg ccattggaat
     2281 attagtacca ttacgcataa attgcatgac catttctaat tcattcattc cttgaccatc
     2341 aagatcaaat gtcaatgcat aaatcatact cgcattttca ttcttaactc gtttacctat
     2401 aaatgaacca cctctaacaa atatatcctc ttttccaatc caattcttaa tctcctctaa
     2461 atcatcaaat atatatctat gcttaaattt accttcattc ctatacaata aaatcccatt
     2521 accacattta cgactataat ctcctttaac ttgaaatgat cctattggat ataaatctct
     2581 ataaaaagct aacggttcta cttctacata atcatgctcc ttaaaccaat taactttagc
     2641 atcatataac tctgtatctg tcatcacaat atcactcata aatacctcta acttaactta
     2701 attatctgag agcccctaag gggctcatag gggacttata ttatcaatga attttttttg
     2761 tacattttac taaaaaaata tcactcataa atacctctaa cttaacttaa ttatctgaga
     2821 gcccctaagg ggctcatagg ggacttatat tatcaatgaa ttttttttgt acatttttag
     2881 tatattaaaa atggtctatt tttaccatca ttttttactt attattcagg agcccctaca
     2941 gggctcatag gggacttata ttatcaatga attttttccc tatcttaagc aacaatataa
     3001 tcaataagtt ttttttacat ttttcatgac aaaattagaa aatcacttta acataccaaa
     3061 gcgattttct aatttacata tatgctataa tttaatcgtt gcctacccta gactggtaac
     3121 aggaaggggg tgctaatttt gaatttacta ttatctttct tactgtctat catggcaagt
     3181 atagccgctt actatatttg caaatggatt gatagacact tctaggcaac aatcactagt
     3241 ggatccacag gacacaatat ccacttgtag tttataataa ctatctcctc ctttacactt
     3301 taattcaaat ctttattaaa aaatatttca tcttatttaa caagaaacca tatttatata
     3361 acaacataaa atacactaag ttattttatt gaacatatat cgtactttat ctatccgact
     3421 atttggacga cggggctggc aaacaggttc accggtagta acatggtacc cttttaactc
     3481 tgttaaacaa acactacgtc catttgtaaa gaaagttaaa tcactacgat attcttgaat
     3541 acaccgagca gggatttctc cactaagaat gacctcatta tttttcaatt gagtgtctac
     3601 gatgttcgca caatatttag gagcatcgtt gtatgctcgt gaaagatatt cctgtggcgc
     3661 ataaatttta aaactaagat atggctctaa caattctgtt ccagcttttt ttaagacttg
     3721 ttccaataca ataggagcaa gcatccgaaa atctgctggg gtactaacag ggctatagta
     3781 taagccatac ttaaaacaga ttttacagtc cgtcacattc caaccataca atccttgttc
     3841 acagccatag cgtatcccct ccataactgc attttgaaac gattgattta agtatccaag
     3901 agaaaccgag ctctcatact gtactccgct ccctaatgga agctgtgcta cagatagacc
     3961 aatggaagcc cagaaaggat tcggtggaac ttcgatgtga atggtatact ctgctttttt
     4021 taacggtctt tccatataaa tgactgtagg ctcttttatt tctatctcca catgatactt
     4081 ttcttgcagc agagcacaag tcacttccat ttgtactttc cctaagaaag aaagtatgat
     4141 ttcatgtgtc gcagaatcca cataatatcg cagaagcggg tcactgtcgg agatttctaa
     4201 aagtgcatca agtaacattt ccctttgttg aggtttgctc ggttcaacag tcgtttgcag
     4261 cagagggagg ggattttcaa ttctctctct ctgtggcaat agctttgtat ctccaagaac
     4321 actatttaac ttcaaaaact cattctgcaa aataacaatt tccccggaat aagccttatc
     4381 gattttacat aattcaccat ttattgaagt atacatttct gtaattttta ttttttcctt
     4441 ttccgatatt ctaaccgaat ctcgcaaatg cagtacgcca ctataaagac gtatatatgc
     4501 aagacgctgt cttttttccg aatactcaat tttgaaaact tttccgcaaa gttcagactg
     4561 acctcgatgt gttgatgaat aaaatttatt cgtaatcact tctataaggt tatcaatccc
     4621 tatattgttt tttgcacttc cgtgataaac agggaacagg gaacaattat gaaatcttat
     4681 gctttcctct tgttcgagtt ctaatgcttc caataatttc ccagacgtat atttctccaa
     4741 aaggtaatca tttccttcta ttaccatatc ccattgttca gattcggtaa agttcattac
     4801 acgcatatta ggatgcagtt ctaccttctg tttgattaca atttccgcag aaagtttctc
     4861 tttaatatcc tgataaaccg ttgataaatc aattccattt tggtcaatct tattgataaa
     4921 aaagattgtg ggaataccta ttttcctaag tgcatgaaac aatatacgag tttgtgcttg
     4981 tacgccatct tttgcagaaa tcagtagaat tgccccatct aatactgata atgaacgata
     5041 tacttctgct aaaaaatcca tatgtcctgg cgtgtctatg atgttcacct tagtattttt
     5101 ccactgaaaa gaggttatcg ccgtctgaat tgtaattcct ctctgacgtt ctaaaagcgt
     5161 attatccgtt ttcgttgtac ctctgtccac gcttcctaat tctgtaatcg ctccactgtt
     5221 atataataag ctttctgtta aggtagtttt tcccgcatca acatgagcta aaactccaat
     5281 attaataatt ttcataatct cacctcattt tactaacact gatactaatt catcatataa
     5341 ttatagcatt tttaagggtt taacgctatc ttttatgtct atttacatac taaatatcgg
     5401 ttatatcgag ctatcatttt taatgtatta ttaatggaat atatttcaaa ttattcttgt
     5461 taacagcgca attccttaag aaaattttct tcactcgcct gcattttact gcaggaattc
     5521 gatatcaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccca gcttttgttc
     5581 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     5641 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     5701 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     5761 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     5821 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     5881 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     5941 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6001 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6061 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6121 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6181 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6241 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6301 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6361 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6421 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     6481 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6541 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6601 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6661 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6721 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6781 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     6841 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     6901 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     6961 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7021 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7081 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7141 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7201 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7261 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7321 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7381 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7441 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7501 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7561 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7621 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7681 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7741 tccgcgcaca tttccccgaa aagtgccac
//
